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All Journal Jurnal Ilmiah Farmasi Berkala Ilmiah Kedokteran Duta Wacana Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal
Ning Rintiswati
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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PREVALENSI VIRURIA SITOMEGALOVIRUS PADA POPULASI ORANG DEWASA NORMAL Dita Ria Selvyana; Praseno Praseno; Ning Rintiswati
Jurnal Ilmiah Farmasi Vol. 2 No. 2 (2005)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTThe aim of this study was to get cytomegalovirus viruria prevalence data in healthy adultpopulation. Urine samples were obtained from healthy individuals. Preparation of sample and sera(polyclonal antibody) were performed by standard method., and detection of cytomegalovirusantigen in urine sediment were done by Indirect Immunofluorescence Antibodi Technique (IFAT).The results were analyzed with chi square pearson. From 341 healthy individuals (16 ♀ and 125 ♂)age between 18 to 61 years. Cytomegalovirys viruria were found in 5 (3, 55%) of subjects. In groupof subjects less than 30 years of age, cytomegalovirus viruria were found in 3,82% subject whereasin group of subjects more than 30 years of age, cytomegalovirus viruria was not found; chi squarepearson statistic analysis did not show significant difference between two groups (p = 0,529). Ingroup of women, cytomegalovirus viruria were found in 6,25% subjects whereas in group of men,cytomegalovirus viruria were found in 3,2% subjects; chi square pearson statistic analysis did notshow significant difference between two groups (p = 0,535). Cytomegalovirus viruria prevalenceamong healthy adult population studied was relatively low (3,55%).Key words: Cytomegalovirus, viruria, prevalence, latent infection, Indirect ImmunofluorescenceAntibodi Technique (IFAT)
GENOTYPE AND RESISTANCE PATTERNS OF ANTI TUBERCULOSIS TREATMENT (ATT) IN Mycobacterium tuberculosis ISOLATES FROM TUBERCULOSIS CASES WHICH NOT TAKEN ATT Maria Silvia Merry; Ning Rintiswati; Yanri Wijayanti S
Berkala Ilmiah Kedokteran Duta Wacana Vol 1, No 2 (2016): BERKALA ILMIAH KEDOKTERAN DUTA WACANA
Publisher : Faculty of Medicine Universitas Kristen Duta Wacana

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Background: Tuberculosis (TB) is still a prominent health problem which need to be controlled worldwide. In Indonesia, the incidences of TB cases in 2011 were 450.000 cases with mortality rate 175 person per day. The emergence of mycobacterium’s resistance against Anti Tuberculosis Treatment (ATT) gives a double burden to prevent the disease. This resistance against ATT is caused by several things, one of which is the nature of mycobacterium, mutations and genotype strain variation. Objective: The aim of this study is to get a description of ATT’s resistance pattern, genotype of M. tuberculosis, and determined the correlation between M. tuberculosis’ genotypes and the resistance pattern against ATT. Methods: The research methods were cross-sectional and analytical descriptions. Samples used in this research were clinical isolates, which were taken from patients who hadn’t received ATT therapy before. Patients were recruited from BP4 (Balai Pengobatan Penyakit Paru = Health Center for Lung’s Diseases) at Minggiran and Kotagede area, for the period of June 2010 - December 2010. Drug susceptibility test for ATT were done for Isoniazid, Rifampicin, Streptomycin, and Ethambutol using LJ’s proportion method. Whereas for genotyping, we were using PCR-based Spoligotyping, with Dra and Drb primers. Data processing for genotypes and resistance pattern were in descriptive form, while the analysis of the ATT resistance and genotypes correlation were using chi square. Results: From 33 samples collected and tested for resistancy, 17 samples (51,52%) were sensitive to INH, RIF, STREP, and ETAMB while 16 samples (48,48%) were resistant to one or more ATT. We found 1 isolate (3.03%) was MDR TB. Genotype patterns description are 30% (10 isolates) were Beijing strain and 70% (23 isolates) were Non Beijing with a variety of EAI, LAM, U, Harleem, T, Manu, and Miscellanous. The chi square’s analysis results are p = 0,034 (p < 0,05), ratio prevalence 2,96 (95 CI 0,26 – 0,57). Conclusion: The result from drug susceptibility test for ATT are 48,48%, resistant to one regimen or more ATT, while sensitivity 51,52%. Beijing strains were predominant strain (30%). There were significant correlation between the patterns of resistance against ATT and genotype patterns, Beijing strains tend to be resistant by 2,96 times greater than non-Beijing strains.
Faktor-Faktor Yang Berpengaruh Terhadap Kejadian Tuberkulosis Pada Wanita di Kabupaten Cilacap Provinsi Jawa Tengah Nana Sumarna; Ning Rintiswati; Lutfan Lazuardi
Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal Vol. 4 No. 2 (2015): Jurnal Ilmu Kesehatan Bhakti Husada
Publisher : Lembaga Penelitian Sekolah Tinggi Ilmu Kesehatan Kuningan

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Background : Tuberculosis (TB) is disease a second major cause of deaths worldwide among infectious diseases, killing nearly 2 million people each year. The variety of problems will arise if the woman was suffering TB especially who are married, pregnant, and have children. Her role as a housewife who had to carry out physical or mental care of children while taking care of her husband will be disturbed. The prevalence of smear positive pulmonary Tuberculosis (TB) in 6 region health center in Cilacap District at 2012, women higher than men.Objective : To know probability of pregnancy, marital status, parity, physical activity, level of education, level of knowledge, the kitchen smoke pollution, history contact with TB patient, residential density, ventilation against TB incidence in women.Method : The type of research was observational analytical case-control design. Sample is 102 people consisting of as many as 51 cases and 51 control. Sampling is done with proportional random sampling techniques. Data were analyzed with the univariabel, bivariabel analysis, and multivariable. Results : Pregnancy (OR 1.2 95% CI 2.4-23.7 p0.04), parity (OR 3.5, 95% CI 1.3-9.7 p 0.01), history of contacts (OR 3.8 95% CI 1.4-10.4 p 0.01) ventilation (OR 2.4 95% CI 7,5-23.7 p 0.00) as a risk factor for the occurrence of TB in women. Conclusion : The incidence of TB in women in Cilacap District a more probable or risk greater in women who were pregnant, high parity, had a history of contacts, and stay at home with the bad ventilation.
Pengaruh Pelatihan Mikroskopis Tuberkulosis Terhadap Kualitas Sediaan dan Slide Positivity Rate di Wilayah Kerja Dinas Kesehatan Kabupaten Purbalingga Dede Kurniawan; Ning Rintiswati; Dibyo Pramono
Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal Vol. 4 No. 2 (2015): Jurnal Ilmu Kesehatan Bhakti Husada
Publisher : Lembaga Penelitian Sekolah Tinggi Ilmu Kesehatan Kuningan

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Introduction: Microscopic examination of sputum is a key component of the diagnosis of tuberculosis. In Purbalingga district, was conducted in 24 peripheral laboratories. Five technicians were trained in 2011-2012 and 12 technicians in 2003-2009 by National Tuberculosis Programs. Seven technicians have not trained. In 2011 slide positivity rate an average of 9.1%, 73.4% poor smear quality and 11.5% poor staining, 1.4% error rate at 34.7% laboratoriy. In 2012 slide positivity rate an average of 8.9, evenness was the highest of poor smear quality (77.7%), and 4.6% error rate at 66,7% laboratory. Therefore, need refresher training and initial training. Objective: To determine impact of training to smear quality and slide positivity rate. Methods: The research design was analytic quasi experimental with 5 days microscopic training. The research subject were 18 technician microscopic. They were divided into experimental and control group each 9 technicians with purposive sampling. The differences of smear quality and slide positivity rate were analyzed by independent t-test and Mann-Whitney test with 95% CI. Results: Training has increased the average of knowledge test score 40.7 points and average of test score the good smear quality : specimen quality increased 90 points, staining 84.4, cleanness 85.6, thickness 91.1, smear size 88.9, evenness 87.8 and increase the average of smear readings score test 22 points. The mean of smear quality and slide positivity rate of the experimental group were higher than control group. Statistical test of smear quality between eksperimental group with contol group were: specimen quality p=0.03, staining quality p=0.03, cleanness p=0.02, thickness p<0.001, size p<0.001, good evenness p<0.001, and slide positivity rate p=0.02 Conclusion: The mean of smear quality and slide positivity rate of the experimental group were higher than control group, there were significant differences of smear quality and slide positivity rate between experimental group with control group at 3 months after training.
Co-Authors Dede Kurniawan Dibyo Pramono Dita Ria Selvyana Lutfan Lazuardi Maria Silvia Merry Praseno Praseno Yanri Wijayanti S