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All Journal Indonesian Journal of Forestry Research Indonesian Journal of Forestry Research
Arif Nirsatmanto
Center for Forest Biotechnology and Tree Improvement, Jl. Palagan Tentara Pelajar Km. 15, Purwobinangun, Yogyakarta 55582 - Indonesia
Agriculture, Biological Sciences & Forestry

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GENETIC GAIN AND PROJECTED INCREASE IN STAND VOLUME FROM TWO CYCLES BREEDING PROGRAM OF Acacia mangium : IMPLICATIONS TO PLANTATION PRODUCTIVITY AND SUSTAINABLE FORESTRY IN INDONESIA Nirsatmanto, Arif
Indonesian Journal of Forestry Research Vol 2, No 2 (2015): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.115 KB) | DOI: 10.20886/ijfr.2015.2.2.71-79

Abstract

Two cycles breeding program of Acacia mangium was practiced by Center for Forest Biotechnology and Tree Improvement Research. Although improved seed from the breeding program have been used in operational plantation, the real amount of gains in productivity was not verified together yet.  This study was aimed to observe realized genetic gain and projected increase in stand volume from the two cycles breeding of A. mangium, and to discuss the implications on plantation productivity and sustainable forestry in Indonesia. Improved seed from first and second-generation seed orchard were tested together with an unimproved seed in genetic gain trial in West Java, with spacing of 3 x 3 m. Measurements were done at three years ages for height, dbh, and stem volume. Realized genetic gain was calculated as the percentage increase of improved seed over the unimproved one. Results of study showed that improved seed performed better growth than the unimproved with realized gain of 5-24% (height), 3-44% (dbh) and 11-90% (stem volume). Improved seed from second-generation outperformed that from the first-generation, with an improvement of 6-16% (height), 3-26% (dbh) and 20-53% (stem volume). Genetic gains increased with increasing ages for height, but it tended to decrease for dbh and stem volume. At given site and silvicultural practices, projected increase in stand volume at 8 years rotation reached 290-325 m3/ha, which is equal to 30-50% of gains. The uses of high genetically improved seed, in combination with intensive silviculture, would provide significant impacts on plantation productivity and sustainable forestry in Indonesia.
GENETIC TRANSFORMATION OF Melia azedarach L., USING Agrobacterium MEDIATED TRANSFORMATION Nirsatmanto, Arif; Gyokusen, Koichiro
Indonesian Journal of Forestry Research Vol 4, No 1 (2007): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2007.4.1.1-8

Abstract

This study was subjected to observe the possibility of  introducing specific foreign genes into Melia azedarach L., using Agrobacterium mediated transformation. Agrobacterium tumefaciens used in this study consisted of  strains of  EHA105 (vector plasmid pBIsGFP) and EHA105 (vector plasmid pBsGFP) to observe the possibility of introducing genes, and strains of EHA101 (vector plasmid pIG121-Hm) and LBA4404/ferritin (vector plasmid pBG-1) to observe the shoot organogenesis after genes transformation. Explants were collected from one cm in length excised stem of in-vitro plantlets. The results of the study showed that genetic transformations of M. azedarach could be potentially developed using Agrobacterium tumefaciens strains : EHA105 (pBIsGFP or pBsGFP), EHA101 (pGI121-Hm) and LBA4404/ferritin (pBG-1). The expression of  GFP (green fluorescence protein) signal worked successfully in this transformation  with 40% of transformation  rate for pBIsGFP and 20 % for pBsGFP.  The application of Agrobacterium strains of EHA101 (pIG121-Hm) and LBA4404/ferritin (pBG-1) which contained specific gene of kanamycin resistance and iron accumulation for plant growth improvement showed that adventitious shoot was well induced and elongated on the rate of 30 - 60 % of explants after genes transformation.
REALIZED GENETIC GAIN AND SEED SOURCE X SITE INTERACTION ON STAND VOLUME PRODUCTIVITY OF ACACIA MANGIUM Nirsatmanto, Arif; Setyaji, Teguh; Wahyuningtyas, Reny Setyo
Indonesian Journal of Forestry Research Vol 1, No 1 (2014): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2014.1.1.21-32

Abstract

Following the results of the comprehensive tree improvement programs for Acacia mangium, it is necessary to estimate the real amount of genetic improvement and to develop an improved seed deployment strategy.  This study was aimed to verify realized genetic gain on stand volume productivity attained by the first-generation Seedling Seed Orchards (SSO) of A. mangium and to identify the magnitude of seed sources x site interaction as a basis for improved seed deployment. Seeds from five SSOs were tested together with seeds from seed stand in genetic gain trials which were established in South Kalimantan and Central Java. Realized gains were calculated from the percentage improvement of respective SSOs compared with seed stand at two and four years of age. Seed source x site interaction was investigated through analysis across the two sites.  Results of the study showed that trees derived from the five SSOs produced better stand volume than those from seed stand. At four years of age, stand volume of the best SSO reached around 127 m3/ha in South Kalimantan and 84 m3/ha in Central Java.  Realized genetic gain were around 66% at two years and 59% at four years in South Kalimantan, and around 136% at two years and 81% at four years in Central Java.  Seed source x site interaction was not significantly different indicating superiority of improved seed were consistent across the two sites with the average realized gain ranging from 18% to 79% and 24% to 62%  at two and four years, respectively. The best three SSOs were SSO-1 located in Pleihari-South Kalimantan, SSO-2 in Pendopo-South Sumatra and SSO-5 in Wonogiri-Central Java, all of which  originated from Papua New Guinea provenances. Improved seed from the orchards could be used at plantation sites without any significant change of the order in their superiority for stand volume productivity.
GENETIC GAIN AND PROJECTED INCREASE IN STAND VOLUME FROM TWO CYCLES BREEDING PROGRAM OF Acacia mangium Nirsatmanto, Arif; Setyaji, Teguh; Sunarti, Sri; Kartikaningtyas, Dwi
Indonesian Journal of Forestry Research Vol. 2 No. 2 (2015): Indonesian Journal of Forestry Research
Publisher : Association of Indonesian Forestry and Environment Researchers and Technicians

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2015.2.2.71-79

Abstract

Two cycles breeding program of Acacia mangium was practiced by Center for Forest Biotechnology and Tree Improvement Research. Although improved seed from the breeding program have been used in operational plantation, the real amount of gains in productivity was not verified together yet. This study was aimed to observe realized genetic gain and projected increase in stand volume from the two cycles breeding of A. mangium, and to discuss the implications on plantation productivity and sustainable forestry in Indonesia. Improved seed from first and second-generation seed orchard were tested together with an unimproved seed in genetic gain trial in West Java, with spacing of 3 x 3 m. Measurements were done at three years ages for height, dbh, and stem volume. Realized genetic gain was calculated as the percentage increase of improved seed over the unimproved one. Results of study showed that improved seed performed better growth than the unimproved with realized gain of 5-24% (height), 3-44% (dbh) and 11-90% (stem volume). Improved seed from second-generation outperformed that from the first-generation, with an improvement of 6-16% (height), 3-26% (dbh) and 20-53% (stem volume). Genetic gains increased with increasing ages for height, but it tended to decrease for dbh and stem volume. At given site and silvicultural practices, projected increase in stand volume at 8 years rotation reached 290-325 m3/ha, which is equal to 30-50% of gains. The uses of high genetically improved seed, in combination with intensive silviculture, would provide significant impacts on plantation productivity and sustainable forestry in Indonesia.
REALIZED GENETIC GAIN AND SEED SOURCE X SITE INTERACTION ON STAND VOLUME PRODUCTIVITY OF ACACIA MANGIUM Nirsatmanto, Arif; Setyaji, Teguh; Wahyuningtyas, Reny Setyo
Indonesian Journal of Forestry Research Vol. 1 No. 1 (2014): Indonesian Journal of Forestry Research
Publisher : Association of Indonesian Forestry and Environment Researchers and Technicians

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2014.1.1.21-32

Abstract

Following the results of the comprehensive tree improvement programs for Acacia mangium, it is necessary to estimate the real amount of genetic improvement and to develop an improved seed deployment strategy. This study was aimed to verify realized genetic gain on stand volume productivity attained by the first-generation Seedling Seed Orchards (SSO) of A. mangium and to identify the magnitude of seed sources x site interaction as a basis for improved seed deployment. Seeds from five SSOs were tested together with seeds from seed stand in genetic gain trials which were established in South Kalimantan and Central Java. Realized gains were calculated from the percentage improvement of respective SSOs compared with seed stand at two and four years of age. Seed source x site interaction was investigated through analysis across the two sites. Results of the study showed that trees derived from the five SSOs produced better stand volume than those from seed stand. At four years of age, stand volume of the best SSO reached around 127 m3/ha in South Kalimantan and 84 m3/ha in Central Java. Realized genetic gain were around 66% at two years and 59% at four years in South Kalimantan, and around 136% at two years and 81% at four years in Central Java. Seed source x site interaction was not significantly different indicating superiority of improved seed were consistent across the two sites with the average realized gain ranging from 18% to 79% and 24% to 62% at two and four years, respectively.
EARLY GROWTH AND STAND VOLUME PRODUCTIVITY OF SELECTED CLONES OF Eucalyptus pellita Setyaji, Teguh; Sunarti, Sri; Nirsatmanto, Arif
Indonesian Journal of Forestry Research Vol. 3 No. 1 (2016): Indonesian Journal of Forestry Research
Publisher : Association of Indonesian Forestry and Environment Researchers and Technicians

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2016.3.1.27-32

Abstract

Using current technologies, several forest plantation companies in Indonesia are pursuing clonal forestry program with E. pellita to increase plantation productivity using selected clones. This paper evaluates the early growth and stand volume productivity of selected clones of E. pellita as part of a breeding program for pulpwood. Two clonal trials of E. pellita were established in Central Java with two different plot configurations: single tree-plot and multiple tree-plot. Trial evaluation was done at two years age involving tree height, diameter, stem volume and stand volume. Result show that among the clones there were significant differences for all traits assessed. All of the tested clones exceeded the control seedling of F-1 generation by 9-50% for height, 10-36% for diameter and 22-137% for stem volume, respectively. Clonal repeatability ranged from 0.7-0.9, with corresponding individual ramet repeatability ranged from 0.2-0.4. Overall stand volume productivity at given age reached 15 m³/ha.
Co-Authors Dwi Kartikaningtyas, Dwi Koichiro Gyokusen, Koichiro Reny Setyo Wahyuningtyas Sri Sunarti Surip Surip Susumu Kurinobu SUSUMU SHIRAISHI Teguh Setyaji