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All Journal Jurnal Ilmu Ternak Veteriner
Koji Niwa
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Veterinary

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Successful ICSI depends on DNA stability and disulfide status of sperm nuclei Said, Syahruddin; Niwa, Koji
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 3 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.67 KB) | DOI: 10.14334/jitv.v9i3.411

Abstract

Relation of successful ICSI (intracytoplasmic sperm injection) with DNA stability and thiol-disulfide status of rat spermatozoa nuclei was investigated. Testicular, caput and cauda epididymal spermatozoa have been used for this research. The nuclei of these spermatozoa were stained with aeridine orange for DNA stability test and labeled with mBBr for thiol-disulfide status before injesting into oocytes. Cauda epididymal spermatozoa were very stable and contained high disulfide than caput epididymal and testicular spermatozoa. Results from spermatozoa injection into oocytes show that they were capable to fertilize oocytes. However, testicular spermatozoa can not develop up to blastocyst stage. These results indicated that successful ICSI at least in the rat depends on DNA stability and disulfide status of sperm nuclei.   Key words: ICSI, DNA stability, disulfide status, acridine orange, mBBr
Successful ICSI depends on DNA stability and disulfide status of sperm nuclei Syahruddin Said; Koji Niwa
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 3 (2004): SEPTEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.67 KB) | DOI: 10.14334/jitv.v9i3.411

Abstract

Relation of successful ICSI (intracytoplasmic sperm injection) with DNA stability and thiol-disulfide status of rat spermatozoa nuclei was investigated. Testicular, caput and cauda epididymal spermatozoa have been used for this research. The nuclei of these spermatozoa were stained with aeridine orange for DNA stability test and labeled with mBBr for thiol-disulfide status before injesting into oocytes. Cauda epididymal spermatozoa were very stable and contained high disulfide than caput epididymal and testicular spermatozoa. Results from spermatozoa injection into oocytes show that they were capable to fertilize oocytes. However, testicular spermatozoa can not develop up to blastocyst stage. These results indicated that successful ICSI at least in the rat depends on DNA stability and disulfide status of sperm nuclei.   Key words: ICSI, DNA stability, disulfide status, acridine orange, mBBr
Co-Authors Syahruddin Said