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All Journal AcTion: Aceh Nutrition Journal Acta Biochimica Indonesiana
Novi Sylvia Hardiany
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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ELISA method to detect ABO blood group in external secretion fluids Abdul Halim Sadikin; Yefta Moenadjat; Novi Sylvia Hardiany
Acta Biochimica Indonesiana Vol. 2 No. 1 (2019): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v2i1.33

Abstract

Background: Usually it takes a large number of volume sample to determine blood group from external secretion fluids. But, in certain condition, samples are only available in very small amount. The objective of this study is to detect the presence of ABO blood group substances in mucosal fluid using ELISA technique, thus only requires small amount of samples. Objective: To develop an ELISA technique using the current anti-ABO antibodies for determination of blood group by hemagglutination technique and second peroxidase label antibody specific for mouse IgG, originally used for another ELISA technique. Methods: 100 μl of diluted human intestinal mucosal fluid were incubated overnight in 4oC in ELISA microplate wells, followed by addition anti-ABO antibodies. Then after incubation, a second revealing antibody anti mouse IgG labeled with peroxidase was added. After a brief incubation, substrate H2O2 and chromogenic TMB were added. Results: Positive reaction is marked by development of blue colour, which, on termination enzymatic reaction by addition 100 μl H2SO4 change to yellow. Conclusion: An ELISA method for detecting ABO substance in mucosal fluid can be developed from antibodies not specifically made for this technique, but specific only for the target.
Determinants of household food security during the COVID-19 pandemic in Tulungagung, East Java Novianti Tysmala Dewi; Dwi Nastiti Iswarawanti; Novi Sylvia Hardiany
AcTion: Aceh Nutrition Journal Vol 8, No 1 (2023): March
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v8i1.761

Abstract

Food insecurity remains a significant problem in Indonesia, with severe levels of hunger, due to inadequate food consumption and poor quality. Since the COVID-19 pandemic, changes in the food system and possible income losses have threatened household food security in Indonesia. This study aims to assess the determinants of household food security during the COVID-19 pandemic. This descriptive-analytic research method with a cross-sectional study was conducted from May to July 2021. The sampling technique used random sampling to obtain two villages with 187 parents in Tulungagung, East Java. Data collection on sociodemographic characteristics, social assistance, the impact of COVID-19, coping strategies, and household food security used interview techniques via WhatsApp and telephone. Testing in this study used chi-square for bivariate analysis and multiple logistic Regression for multivariate analysis. The results showed that the prevalence of food insecurity in Tulungagung was 56,1%. After adjusting for several potential confounding factors, such as income (p= 0,021; OR= 2,388), type of social assistance (NGO) (p= 0,002; OR= 3,945), change in employment status (p= 0,044; OR= 2,026), and food compromise coping strategy (p= 0,008; OR= 10,134) are determinants of household food security. In conclusion, the determinants of household food security are income, change in employment status, type of social assistance (NGO), and food compromise coping strategies.
Co-Authors Abdul Halim Sadikin Dwi Nastiti Iswarawanti Novianti Tysmala Dewi Yefta Moenadjat