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All Journal Scripta Biologica PSEJ (Pancasakti Science Education Journal)
Hana Hana
Faculty Of Biology, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Physics

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Aspek Biologi dan Lingkungan Polychaeta Nereis sp. di Kawasan Pertambakan Desa Jeruklegi Kabupaten Cilacap: Potensinya Sebagai Pakan Alami Udang Eko Setio wibowo; Endah Sri Palupi; IGA. Ayu Ratna Puspita Sari; Atang Atang; Hana Hana
PSEJ (Pancasakti Science Education Journal) Vol. 3 No. 1 (2018)
Publisher : Program Studi Pendidikan IPA, FKIP Universitas Pancasakti (UPS) Tegal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.771 KB)

Abstract

Cacing Nereis Sp. merupakan hewan invertebrata anggota Familia Nereidae, Classis Polychaeta yang hidupdi ekosistem estuarin, sebagai benthik. Nereis Sp. dapat dimanfaatkan untuk pakan udang karenamengandung asam amino dan asam lemak tak jenuh yang tinggi, untuk menyempurnakan mutu sel gametpada induk udang dan meningkatkan mutu larva. Cacing ini banyak terdapat di daerah pertambakanCilacap tetapi masih belum banyak informasi tentang aspek biologi cacing Nereis Sp. yang di hidup diwilayah ini, sehingga perlu dilakukan studi tentang aspek biologi cacing Nereis Sp. di wilayah ini untukinformasi usaha pengembangan budidaya cacing lur. peneiltian ini dilakukan dengan metode survei.Pengambilan sampel di daerah pertambakan desa Jeruklegi kabupaten Cilacap dalam empat stasiundengan tiga titik pengambilan sampel dan di ulang tiga kali setiap dua minggu sekali. Hasil penelitianmenunjukkan bahwa kondisi aspek biologi cacing Nereis sp. dari empat stasiun pengambilan, yaitu jumlahsegmen; berat tubuh dan rasio kelamin tidak berbeda nyata (p>0,05). Kondisi Lingkungan masihmendukung kehidupan Nereis sp. untuk tumbuh dan berkembang biak
AKTIVITAS TRIPSIN-LIKE DAN KIMOTRIPSIN-LIKE PADA IKAN SIDAT TROPIK Anguilla bicolor McClelland Annisa Fitri Larassagita; Hana Hana; Untung Susilo
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.125 KB) | DOI: 10.20884/1.sb.2018.5.1.660

Abstract

The ability of eel (Anguilla bicolor McClelland) to digest feed protein is highly dependent on the alkaline proteases in the intestine which are trypsin-like and chymotrypsin-like. Information on the trypsin-like and chymotrypsin-like activities is needed to understand the digestive capacity of eels to ingest feed proteins. However, the information on this subject is insufficient. This research aimed to determine the activity of trypsin-like and chymotrypsin-like of eel on the different body weight and incubation temperature. Three groups of eel stadia were selected to represent different body weight, i.e., elver, yellow eel, and pre-silver stadia, and three different enzyme incubation temperatures of 30, 40, and 50°C was applied. The trypsin-like and chymotrypsin-like activity were measured using a spectrophotometry method. The results showed the activities of trypsin-like and chymotrypsin-like were significantly different between the body size and the intestine segment (P < 0.05). The highest trypsin-like and chymotrypsin-like activities were found in the elver stadia approximately 41.25 ± 0.898 g, in the anterior part of the intestine (P < 0.05). The incubation temperatures were not differed significantly (P > 0.05). This study showed the eel had a higher digestive capacity of protein at elver stadium, mainly occur in the anterior part of the intestine, whereas, the temperature ranges from 30 to 50°C had no significant effect on the enzyme activities.
AKTIVITAS PROTEASE DAN AMILASE PADA IKAN SIDAT Anguilla bicolor McClelland) Mohamad Taufik; Hana Hana; Untung Susilo
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.552 KB) | DOI: 10.20884/1.sb.2017.4.3.418

Abstract

This study was experimental, conducted using a Completely Randomized Design (CRD) with 3 x 2 factorial design, and four replicates have been carried out to evaluate the protease and amylase activities of Anguilla bicolor McClelland. A total of 71 individuals divided into three weight groups were used in this study.  The first group with an average weight of 41.25 ± 0.898 g consisted of 51 eels,  the second with an average weight of 319.8 ± 4.666 g composed of 14 eels, and the third with a mean weight of 569.5 ± 9.150 g consisted of 6 eels. The results showed the protease activity differed significantly based on eel size and intestinal segment (P ˂ 0.05). This research recorded the highest protease activity was in eels within the smallest weight group (41.25 ± 0.898 g). This study also revealed the protease activity in the anterior intestine was higher than the posterior in all size of eels. The amylase activity did not differ significantly (P>0.05) by eel size and intestinal segment. This study concluded the protein digestion capacity of smaller eels was higher than larger eels, and the protein digestion capacity was greater in the anterior intestine than the posterior intestine. The carbohydrate absorption capacity in eel was not affected by the variety of fish size which indicates no change in the feed category.
Co-Authors Annisa Fitri Larassagita Atang Atang Eko Setio Wibowo Endah Sri Palupi IGA. Ayu Ratna Puspita Sari Mohamad Taufik Untung Susilo Untung Susilo