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Efficacy of DNA vaccine encoding koi herpesvirus glycoprotein GP-25in common carp juvenile by immersion Nuswantoro, Soko; Alimuddin, .; Yuhana, Munti; Santika, Ayi; Nuryati, Sri; Zainun, Zakki; Mawardi, Mira
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Koi herpesvirus (KHV) is a herpesvirus that particularly infects and causes mass mortality to koi and common carp. Therefore, the protection of common carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine encoding KHV glycoprotein-25 by immersion method to increase survival of common carp against KHV infection. A total of 400 common carp juveniles at 30-day-old were immersed in 1-L water containing 1.3×108CFU/mL of the killed Escherichia coli cells carrying DNA vaccine. Three frequencies and three duration of fish immersion were tested, namely: 1×30 minutes, 1×60 minutes, 1× 90 minutes, 2×90 minutes and 3×90 minutes by interval of 24 hours. Reversetranscription polymerase chain reaction analysis showed that DNA vaccine was successfully expressed in the vaccinated fish. Fish at twenty eight days post vaccination were challenged by injecting 10-4 mL of KHV per fish. The result showed that vaccination by 1×30 minutes immersion allowed 61% of fish survived, and this was significantly higher (p<0.05) compared to control (without vaccination), but it was similar among vaccination treatments (p>0.05). The relative percent survival of vaccinated fish were also similar among treatments (p>0.05). DNA vaccination has increased fish survival about two fold higher compared to unvaccinated fish control (26.67%). Thus, DNA vaccination was effectively delivered by immersion for 1×30 minutes, and this technique can be useful to level up the resistance of common carp juveniles against KHV infection. Keywords: DNA vaccine, KHV, glycoprotein, immersion, common carp

Hatchery Performance of Barramundi (Lates calcarifer) in the Integrated Pond Systems Yudha, Hirmawan Tirta; Santoso, Ratna Amalia; Rahardjo, Seto Sugianto Prabowo; Wibawa, Gigih Setia; Nuswantoro, Soko
Journal of Fish Health Vol. 5 No. 2 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i2.6291

The barramundi, also known as Asian sea bass, has the ability to adapt to various environmental conditions. Indonesia has introduced barramundi from different regions, including Australia, which is known to have a faster growth rate and larger body size. At the same time, the Indonesian strain demonstrates greater resilience to local conditions but grows more slowly. This study evaluates the hatchery performance of Australian and Indonesian strains of barramundi (Lates calcarifer) in a controlled environment using circular concrete ponds. The research examines water quality, spawning productivity, and larval development. The results indicate that the Australian strain produces more eggs than the Indonesian strain, with a hatching rate of 62%. Key water quality parameters, including temperature (28.3–28.6°C), pH (8.26–8.29), and salinity (33–35 ppt), were generally favorable for successful spawning and larval development. Additionally, providing live feed, such as rotifers and Artemia, improved larval growth up to the early juvenile stage. The significant differences in productivity between the two strains highlight the importance of genetic selection and nutritional management in optimizing barramundi hatchery production. This study underscores the need for superior strain selection and the application of science-based aquaculture strategies to enhance the sustainability of barramundi production in Indonesia.

PENGARUH PEMBERIAN PAKAN ALAMI CACING Tubifex sp. TERHADAP PANJANG DAN BERAT IKAN RAMIREZI (Mikrogeophagus ramirezi) Budianto, Mr; Nuswantoro, Soko; Suprastyani, Heny; Ekawati, Arning Wilujeng
JFMR (Journal of Fisheries and Marine Research) Vol. 3 No. 1 (2019): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2019.003.01.10

Salah satu ikan hias yang memiliki prospek perkembangan yang cukup baik adalah ikan ramirezi (Mikrogeophagus ramirezi). Dalam pemeliharaan ikan ramirezi diperlukan perlakuan khusus dalam penanganan benihnya. Tujuan dari penelitian ini yakni dapat mengetahui pengaruh pemberian pakan alami cacing Tubifex sp. terhadap pertambahan panjang dan berat benih ikan Ramirezi. Penelitian ini terdapat 2 perlakuan yakni perlakuan A (pemberian pakan pellet pada pagi dan sore hari) dan perlakuan B (pemberian pakan cacing Tubifex sp pada pagi dan sore hari). Masing-masing perlakuan dilakukan dengan 3 kali ulangan. Pakan pellet dan cacing Tubifex sp yang diberikan masing-masing secara ad libitum selama 14 hari. Hasil pengamatan dan perhitungan menunjukkan bahwa persentase pertambahan benih ikan pada perlakuan B mengalami peningkatan yang signifikan daripada hanya diberi pakan pellet saja (Perlakuan A). Selama 14 hari, pemberian pakan alami cacing Tubifex sp. pada waktu pagi dan sore, benih ikan mengalami penambahan panjang sebesar 13,78 % dan berat sebesar 106,48 %. Hasil ini sangat jauh berbeda dengan benih ikan yang hanya diberi pakan pellet pada pagi dan sore hari, yakni hanya 12,31 % untuk beratnya dan panjangnya tetap. Kesimpulan dari penelitian ini adalah pemberian pakan alami cacing Tubifex sp. berpengaruh signifikan terhadap pertambahan panjang dan berat benih ikan Ramirezi.

Hubungan Laju Penyerapan Kuning Telur dengan Pertumbuhan Larva Ikan Maanvis (Pterophyllum scalare) Nuswantoro, Soko; Pradhana, Anindito; Kusumah, Ruby Vidia; Fariedah, Fani
JFMR (Journal of Fisheries and Marine Research) Vol. 3 No. 2 (2019): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2019.003.02.4

Penelitian ini bertujuan untuk mengetahui hubungan penyerapan kuning telur ikan maanvis (Pterophyllum scalare) pada pertumbuhan larva. Ikan hias mulai diminati oleh masyarakat. Indonesia menjadi salah satu eksportir ikan hias ke luar negeri. Ikan maanvis merupakan ikan yang dapat dibudidayakan dengan pemijahan alami. Pemberian pakan yang tepat dapat mengurangi cost dalam usaha pembenihan dalam fase larva. Metode penelitian ini menggunakan embrio ikan maanvis dengan parameter volume kuning telur dan panjang larva selama 8 hari, diamati setiap 24 jam. Hasil penelitian menunjukkan volume kuning telur yang berkurang diimbangi dengan pertambahan panjangnya ukuran larva. Pada penelitian ini terdapat hubungan laju penyerapan kuning telur dengan pertambahan panjang ukuran larva

Hatchery Performance of Barramundi (Lates calcarifer) in the Integrated Pond Systems Yudha, Hirmawan Tirta; Santoso, Ratna Amalia; Rahardjo, Seto Sugianto Prabowo; Wibawa, Gigih Setia; Nuswantoro, Soko
Journal of Fish Health Vol. 5 No. 2 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i2.6291

The barramundi, also known as Asian sea bass, has the ability to adapt to various environmental conditions. Indonesia has introduced barramundi from different regions, including Australia, which is known to have a faster growth rate and larger body size. At the same time, the Indonesian strain demonstrates greater resilience to local conditions but grows more slowly. This study evaluates the hatchery performance of Australian and Indonesian strains of barramundi (Lates calcarifer) in a controlled environment using circular concrete ponds. The research examines water quality, spawning productivity, and larval development. The results indicate that the Australian strain produces more eggs than the Indonesian strain, with a hatching rate of 62%. Key water quality parameters, including temperature (28.3–28.6°C), pH (8.26–8.29), and salinity (33–35 ppt), were generally favorable for successful spawning and larval development. Additionally, providing live feed, such as rotifers and Artemia, improved larval growth up to the early juvenile stage. The significant differences in productivity between the two strains highlight the importance of genetic selection and nutritional management in optimizing barramundi hatchery production. This study underscores the need for superior strain selection and the application of science-based aquaculture strategies to enhance the sustainability of barramundi production in Indonesia.

Pemanfaatan Kolam Beton Untuk Budidaya Dan Konservasi Lobster Batu (Panulirus penicillatus) Aziz, Abdul; Aisyah, Sitti; Rahardjo, Seto Sugianto Prabowo; Nuswantoro, Soko; Permanasari, Setya Widi Ayuning
Juvenil Vol 6, No 4: November (2025)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v6i4.29042

ABSTRAKLobster batu (Panulirus penicillatus) merupakan salah satu komoditas perikanan bernilai ekonomi tinggi yang memiliki potensi besar untuk dibudidayakan secara intensif dan berkelanjutan. Penelitian ini bertujuan untuk mengevaluasi efektivitas sistem kolam beton berfilter dan program konservasi dalam mendukung pertumbuhan serta pelestarian lobster batu. Studi dilakukan di Kelompok Pembudidaya Ikan “Pesona Bahari” Grand Watu Dodol, Banyuwangi, selama 40 hari, dengan fokus pada analisis tingkat kelangsungan hidup (Survival Rate, SR), pertumbuhan berat dan panjang lobster, efisiensi pakan, serta parameter kualitas air. Hasil penelitian menunjukkan tingkat kelangsungan hidup lobster sebesar 90% dan laju pertumbuhan spesifik (Specific Growth Rate, SGR) mencapai 1,13% per hari. Pemberian pakan berupa kerang hijau dan ikan rucah secara bergantian menghasilkan efisiensi pakan yang tinggi dengan nilai Feed Conversion Ratio (FCR) yang rendah. Parameter kualitas air, seperti suhu, pH, oksigen terlarut, dan salinitas, berada dalam rentang optimal untuk budidaya lobster. Program konservasi yang melibatkan pelepasliaran lobster secara selektif dan edukasi masyarakat memberikan dampak positif terhadap pelestarian sumber daya laut. Namun, diperlukan pemantauan pasca-pelepasliaran dan studi lanjutan mengenai adaptasi lobster di alam untuk meningkatkan efektivitas konservasi. Penelitian ini membuktikan bahwa penerapan teknologi budidaya yang inovatif dan berkelanjutan dapat meningkatkan produktivitas lobster batu sekaligus mendukung pelestarian ekosistem laut. Model budidaya ini dapat direplikasi untuk mengoptimalkan potensi perikanan di wilayah pesisir lainnya.Kata Kunci: Panulirus penicillatus, budidaya lobster, efisiensi pakan, kualitas air, konservasi berkelanjutanABSTRACTThe spiny lobster (Panulirus penicillatus) is a high-value fisheries commodity with significant potential for intensive and sustainable aquaculture. This study aims to evaluate the effectiveness of a filtered concrete pond system and conservation programs in supporting the growth and preservation of spiny lobsters. The research was conducted at Fish Farmers Group “Pesona Bahari” Grand Watu Dodol, Banyuwangi, over 40 days, focusing on survival rate (SR), weight and length growth, feed efficiency, and water quality parameters. The results showed a survival rate of 90% and a specific growth rate (SGR) of 1.13% per day. Feeding spiny lobsters with green mussels and trash fish alternately resulted in high feed efficiency, as indicated by a low Feed Conversion Ratio (FCR). Water quality parameters, including temperature, pH, dissolved oxygen, and salinity, were within optimal ranges for lobster aquaculture. The conservation program, involving selective lobster releases and community education, positively impacted marine resource sustainability. However, post-release monitoring and further studies on lobster adaptation in the wild are needed to improve conservation effectiveness. This study demonstrates that applying innovative and sustainable aquaculture technologies can enhance spiny lobster productivity while supporting marine ecosystem preservation. This aquaculture model can be replicated to optimize fisheries potential in other coastal areas.Keywords: Panulirus penicillatus, lobster aquaculture, feed efficiency, water quality, sustainable conservation

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