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All Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research Bioscientia Medicina : Journal of Biomedicine and Translational Research
Kemas Ya'kub Rahadiyanto
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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The Effect of Ischemic-Reperfusion on Acute Kidney Injury Pathogenesis to the Glomerular Microscopic Appearance and Cystatin C Level in Wistar White Rat. Annisa Wimaulia Azlin; Rachmat Hidayat; Kemas Ya'kub Rahadiyanto
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.79

Abstract

Acute kidney injury (AKI) is a sudden decrease of kidney function. The incidence of AKI is increasing every year. One of the causes of AKI is the lack of blood supply to the kidneys (prerenal). At the present time, there are not many studies that report unilateral ischemic-reperfusion (UIR) duration which can cause kidney damage especially glomerulus. The aim of this study is to determine the effect of UIR duration to glomerular microscopic appearance, GFR and cystatin C produced in Wistar white rats. This study was performed in vivo by using Post-test Only Control Group Design. Unilateral Ischemic-Reperfusion was administered on the rat’s left kidney and recovery was done according to specified time. After recovery time, rat’s blood was taken, the rat was euthanized, and its kidneys were taken and stained with Picrosirius Red coloring. The kidneys were observed by using OptiLED and the photos were analyzed with ImageJ software. Blood samples were tested by ELISA to measure the cystatin C levels and the levels were converted into Larrson formula to obtain Glomerular Filtration Rate. The level of cystatin C increased along with the longer duration of UIR and compared inversely proportional to GFR which decreased along with the rise of UIR duration. Cystatin C and GFR had a significant mean difference (p<0.05) with all groups, except for the duration of the UIR group <60 minutes. The percentage of collagen obtained fluctuated but the whole group which was carried out by UIR had a significantly different collagen amount (p <0.05) with the sham-operated group. The average glomerular picture showed the addition of collagen, Bowman's capsule thickening and vascular retraction. The longer duration of UIR will worsen the kidney function. Keywords: Unilateral Ischemic-Reperfusion, Cystatin C. Glomerular Filtration Rate, Collagen Area Fraction, Glomerulus
The Relationships between Neutrophil/Lymphocyte Ratio and Child-Turcotte-Pugh Classification in Assessing Severity of Liver Cirrhosis Griselda Griselda; Kemas Ya'Kub Rahadiyanto; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.80

Abstract

Background Liver cirrhosis is a disease with high morbidity and mortality in Indonesia. The Child-Turcotte-Pugh classification is a noninvasive parameter to assess severity of liver cirrhosis. Meanwhile, the neutrophil/lymphocyte ratio can reflect person's immunity to liver cirrhosis. This study was conducted to determine the agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in assessing severity of liver cirrhosis. Methods Analytical observational study with Cohen’s Kappa agreement test was conducted in 86 liver cirrhosis patients. Data was retrieved by observing the medical records. Neutrophil count, lymphocyte count, hepatic encephalopathy, ascites, albumin, bilirubin, and INR were all recorded. Documented variables were analyzed by the Cohen’s Kappa agreement test. Results In Cohen’s Kappa agreement test, there was moderate agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in all samples (κ = 0.591 and p <0.001). Agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification based on age and gender was found to be κ = 0.777 at age ≤45 years, κ = 0.532 at age >45 years, κ = 0.682 in male, and κ = 0.445 in female (p <0.001). The highest agreement is the substantial agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification obtained at age ≤45 years (κ = 0.777 and p <0.001) and male (κ = 0.682 and p <0.001). Conclusion There is moderate agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in assessing severity of liver cirrhosis. Substantial agreement obtained at age ≤45 years and male gender. Keywords: Neutrophil/Lymphocyte Ratio, Child-Turcotte-Pugh, Liver Cirrhosis
The Effect of Ischemic-Reperfusion on Acute Kidney Injury Pathogenesis to the Glomerular Microscopic Appearance and Cystatin C Level in Wistar White Rat. Annisa Wimaulia Azlin; Rachmat Hidayat; Kemas Ya'kub Rahadiyanto
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.79

Abstract

Acute kidney injury (AKI) is a sudden decrease of kidney function. The incidence of AKI is increasing every year. One of the causes of AKI is the lack of blood supply to the kidneys (prerenal). At the present time, there are not many studies that report unilateral ischemic-reperfusion (UIR) duration which can cause kidney damage especially glomerulus. The aim of this study is to determine the effect of UIR duration to glomerular microscopic appearance, GFR and cystatin C produced in Wistar white rats. This study was performed in vivo by using Post-test Only Control Group Design. Unilateral Ischemic-Reperfusion was administered on the rat’s left kidney and recovery was done according to specified time. After recovery time, rat’s blood was taken, the rat was euthanized, and its kidneys were taken and stained with Picrosirius Red coloring. The kidneys were observed by using OptiLED and the photos were analyzed with ImageJ software. Blood samples were tested by ELISA to measure the cystatin C levels and the levels were converted into Larrson formula to obtain Glomerular Filtration Rate. The level of cystatin C increased along with the longer duration of UIR and compared inversely proportional to GFR which decreased along with the rise of UIR duration. Cystatin C and GFR had a significant mean difference (p<0.05) with all groups, except for the duration of the UIR group <60 minutes. The percentage of collagen obtained fluctuated but the whole group which was carried out by UIR had a significantly different collagen amount (p <0.05) with the sham-operated group. The average glomerular picture showed the addition of collagen, Bowman's capsule thickening and vascular retraction. The longer duration of UIR will worsen the kidney function. Keywords: Unilateral Ischemic-Reperfusion, Cystatin C. Glomerular Filtration Rate, Collagen Area Fraction, Glomerulus
The Relationships between Neutrophil/Lymphocyte Ratio and Child-Turcotte-Pugh Classification in Assessing Severity of Liver Cirrhosis Griselda Griselda; Kemas Ya'Kub Rahadiyanto; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.80

Abstract

Background Liver cirrhosis is a disease with high morbidity and mortality in Indonesia. The Child-Turcotte-Pugh classification is a noninvasive parameter to assess severity of liver cirrhosis. Meanwhile, the neutrophil/lymphocyte ratio can reflect person's immunity to liver cirrhosis. This study was conducted to determine the agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in assessing severity of liver cirrhosis. Methods Analytical observational study with Cohen’s Kappa agreement test was conducted in 86 liver cirrhosis patients. Data was retrieved by observing the medical records. Neutrophil count, lymphocyte count, hepatic encephalopathy, ascites, albumin, bilirubin, and INR were all recorded. Documented variables were analyzed by the Cohen’s Kappa agreement test. Results In Cohen’s Kappa agreement test, there was moderate agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in all samples (κ = 0.591 and p <0.001). Agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification based on age and gender was found to be κ = 0.777 at age ≤45 years, κ = 0.532 at age >45 years, κ = 0.682 in male, and κ = 0.445 in female (p <0.001). The highest agreement is the substantial agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification obtained at age ≤45 years (κ = 0.777 and p <0.001) and male (κ = 0.682 and p <0.001). Conclusion There is moderate agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in assessing severity of liver cirrhosis. Substantial agreement obtained at age ≤45 years and male gender. Keywords: Neutrophil/Lymphocyte Ratio, Child-Turcotte-Pugh, Liver Cirrhosis
Co-Authors Annisa Wimaulia Azlin Griselda Griselda RACHMAT HIDAYAT