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All Journal Journal of the Indonesian Tropical Animal Agriculture Jurnal Tanah dan Iklim
R. OKTAVIANI
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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CHARACTERISTIC AND RUBBER PLANT REVEGETATION AT THE POST COAL MINING LAND KUTAI KARTANEGARA REGENCY ., SUJIMAN; SITORUS, SANTUN R.P.; OKTAVIANI, R.; ., MACHFUD
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) No 33 (2011): Juli 2011
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v0n33.2011.%p

Abstract

The research objective was to analyse the characteristic of post coal mining land in Kutai Kartanegara Regency and to find out what commodities that suitable for vegetation of this land. The methode of research was using primary data including soil sampling taken with purposive sampling and composite methode. Rubber planting experiment was also carried out at two different soil conditions, that was land with original soil and land withtailing soil (overburden of coal mining). Both soil conditions were employed experimental using randomized completely block design with four treatments, i.e. : (1) without fertilizer, (2) liming, (3) manure fertilizer, and (4) manure fertilizer + liming. Secondary data for commodity research was taken from bureau of statistic centre, labour service, and mining service of Kutai Kartanegara Regency.The result showed that reclaimed land (5-20 years) was classified as S2 class of land suitability for rubber. The competitive commodities of Kutai Kartanegara Regency were rubber, coffea, and coconut, meanwhile strategic commodities were oil palm, pepper, and cocoa. The result of rubber experiment showed that post coal mining land with original soil significantly affective to leaf amount, height, and stem diameter of rubber.
Identification of single nucleotide polymorphism c.957A>C of PLAG1 gene and its association with growth traits in Bali cattle Putra, I. G. R.; Sari, D. A.; Rachmawati, S. M.; Oktaviani, R.; Noor, R. R.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 3 (2021): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.3.199-208

Abstract

The PLAG1 gene is one of the genes that affect the growth traits located on chromosome 14 in cattle. This study aims to obtain SNP of the PLAG1 gene in exon 1 and exon 2 and their association with growth traits in Bali cattle. The number of samples used was 52 samples of Bali cattle, 10 samples of Peranakan Ongole (PO), and 8 samples of Limousine cattle. Identification of SNPs PLAG1 gene was analyzed by direct sequencing method and genotyping of selected SNPs was carried out using PCR-RFLP. Association of genotypes of SNP c.957A>C with growth using t-test. There were 7 SNPs in exon 2 of the PLAG1 gene, namely SNP c.339A>G, c.489C>T, c.795A>G, c.957A>C, c.1023C>T, c.1056A>G, and c.1353A>G. SNP c.957A>C was validated by PCR-RFLP using TaqI enzyme and obtained three genotypes, namely genotypes AA, AC, and CC with allele frequency A and C, respec-tively 0.10 and 0.90 in Bali cattle, while in PO and Limousine cattle were monomorphic. Genotype association of SNP c.957A>C PLAG1 gene were not associated with birth weight (BW0), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365), yearling weight at 730 days of age (YW730), and average daily gain (ADG). SNP c.957A>C as a specific SNP for Bali cattle needs to be investigated in further research as a candidate marker for growth traits in Bali cattle.
Co-Authors Jakaria Jakaria Machfud . Putra, I. G. R. R. R. Noor, R. R. Rachmawati, S. M. Santun R.P. Sitorus Sari, D. A. Sujiman .