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All Journal Edubiotik : Jurnal Pendidikan, Biologi dan Terapan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medical Laboratory Analysis and Sciences Journal Borneo Journal of Medical Laboratory Technology
Eka Puspitasari
STIKes Hutama Abdi Husada Tulungagung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Social Sciences

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The Potential Use of EDTA as an Alternative to Defibrination in Preparing Blood Agar Plates with Human AB Blood Type on Staphylococcus aureus Culture Dora Dayu Rahma Turista; Eka Puspitasari; Fanny Kurnanda Razvi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1923

Abstract

Blood Agar Plates (BAP) are composed of blood as one of the compositions. Sheep’s blood is usually used, but since it is difficult to be obtained, human AB blood type was used as an alternative. In preparing BAP, blood is defibrinated to lyse the blood clotting factors. Blood clots can also be prevented by adding anticoagulants, such as ethylenediaminetetraacetic acid (EDTA). This study aims to investigate the potential use of EDTA as a substitute for defibrination in preparing BAP with human AB blood type. This study employed a completely randomized design with true experimental method using Staphylococcus aureus as the sample. The parameters were the number of colonies, types of hemolysis, and hemolysis zone. The results showed that the S. aureus grown on BAP with EDTA-human AB blood type was 64 colonies (mean), produced β-hemolytic pattern, and 6 mm hemolytic zone. In contrast, the S. aureus grown on BAP with defibrinated human AB blood type showed 82 colonies (mean), β-hemolytic pattern, and 5 mm hemolytic zone. There were significant differences in the number of colonies (0.000 < α) and hemolytic zones (0.02 < α). However, there was no difference in the hemolysis type (both treatments produced β-hemolysis). EDTA was possible to be used as a substitute for defibrination in preparing BAP to assess the hemolysis type of S. aureus, but it might not be able to be used as a benchmark for counting the number of colonies and determining the hemolysis zone of S. aureus.
Identifikasi Sedimen Urine pada Penduduk yang Mengkonsumsi Air Sumur di Desa Besole Kecamatan Besuki Kabupaten Tulungagung Chalies Diah Pratiwi; Eka Puspitasari
Borneo Journal of Medical Laboratory Technology Vol 1 No 2 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.9 KB) | DOI: 10.33084/bjmlt.v1i2.711

Abstract

Drinking water that is consumed should be clean and healthy. To avoid illness and disruption of bodily functions such as kidney function, liver, brain, teeth, and even mental disorders. Some people in Besole village still use dug well water as a source of drinking water. The geographical state of the town of Besole, Tulungagung district, is a mountainous area of marble containing lime which allows for mineral content, main calcium in the drinking water of residents. This study aims to determine the identification of urine sediments in residents who consume well water in Besole Village, Besuki District, Tulungagung Regency in 2018. This type of research is descriptive non-analytic, by determining the percentage of urine sediments that consume well water through an examination at the Pathology Laboratory STIKes Hutama Abdi Husada Tulungagung. The number of samples is 30 people, with simple random sampling technique. Based on the research conducted found the results of abnormal urine sediment percentage, ie, 6.7% leukocytes, 3.3% erythrocytes, 16.7% epithelium, 30% calcium oxalate and 3.3% bacteria. Water containing lime should be boiled, precipitated and filtered before consumption.
Deskripsi Jumlah Leukosit dan Laju Endap Darah pada Pasien Tuberkulosis di Rumah Sakit Paru Dungus Madiun Chalies Diah Pratiwi; Eka Puspitasari; Vivi Nurohmah
Borneo Journal of Medical Laboratory Technology Vol 2 No 1 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.345 KB) | DOI: 10.33084/bjmlt.v2i1.1085

Abstract

Tuberculosis is an infectious disease of global concern caused of bacterium Mycobacterium tuberculosis. It can attack lung, liver, kidneys, bones or skin. If a person infected by tuberculosis, the body will begin the mechanism of inflammation as a form of haematological manifestation. The purpose of this study was to describe the number of leukocytes and Blood Sedimentation Rate (BSR) in tuberculosis patients. The research design used is non-analytic descriptive. The population in this study were all tuberculosis patients at the Dungus Madiun Lung Hospital in 2018Using the Quota Sampling technique, the sample in this study was 30 respondents. This research was conducted on May 2018. The results of the study on tuberculosis patients showed that the number of leukocytes in TB patients was 9120 cells/µL blood, 11 (37%) patients had leukocytosis and 19 (63%) sufferers have a nominal leukocyte count. The lowest leukocyte count was 4100 cells/µL of blood and the highest was 24700 cells/µL of blood. The average value of BSR in TB patients was 71 mm/hour I, 29 (96.6%) patients experienced an increase in BSR values and 1 (3.4%) patients had normal BSR values. The lowest value of BSR is 11 mm/hour I and the highest value of BSR is 106 mm/hour I. Prevention of tuberculosis infection can be done by conducting laboratory diagnosis to find out how far the disease goes and choose the right OAT for patients. With intensive treatment, it will reduce the number of leukocytes and reduce the risk for MDR TB
Review: Perbedaan Kadar Hemoglobin Menggunakan Hematologi Analyzer dan Spektrofotometer pada Ibu Hamil Andyanita Hanif Hermawati; Eka Puspitasari; Deny Yusnia Milasari
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2388

Abstract

Hemoglobin is the main component of red blood cells, a conjugated protein that functions to transport oxygen and carbon dioxide. Hemoglobin examination can be done using a hematology analyzer and spectrophotometry. This study aims to determine the data analysis whether there is a difference in hemoglobin levels using a hematology analyzer and spectrophotometry in pregnant women. The method used is Literature Review; the nature of this research is descriptive. The population of this research is health journals that have been through screening, namely journals of differences in hemoglobin levels using a hematology analyzer and spectrophotometry in pregnant women. In this study, the data used are secondary data and data analysis using qualitative data. The results of the evaluation of the study by the Literature Review with hemoglobin levels in pregnant women at Hermina Hospital, Surakarta, showed that the average hemoglobin level of 30 samples of pregnant women using a hematology analyzer was below normal, namely 10.3 gr/dL and normal hemoglobin level is 12.80 gr/dL. The hemoglobin level using spectrophotometry and hematology analyzer on the sample was immediately checked, the results of the hemoglobin levels were 12.98 gr/dL and 11.70 g/dL, respectively. The hemoglobin level showed that the average hemoglobin before being given Fe-Folate with orange water was 11.71 g/dL. The conclusion of this study is the difference in hemoglobin levels is not much different, so there is no difference between the spectrophotometry and the hematology analyzer
Low density lipoprotein (LDL) in type 2 diabetes mellitus Andyanita Hanif Hermawati; Eka Puspitasari; Cholila Lailatul Nurmala
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.065 KB) | DOI: 10.35584/melysa.v1i1.19

Abstract

Hyperglycemia due to abnormal insulin secretion, insulin work or both. Type 2 Diabetes Mellitus is the most among other types of diabetes mellitus. Diabetes mellitus is usually accompanied by dyslipidemia. Dyslipidemia is a disorder of lipid metabolism or cholesterol characterized by an increase or decrease in lipid fraction in the plasma. Diabetes mellitus and elevated Low-Density Lipoprotein (LDL) cholesterol levels are often found to be interrelated. The purpose of this study was to describe Low-Density Lipoprotein (LDL) levels in patients with type 2 diabetes mellitus. The research design used was descriptive non-analytic. The population in this study were all patients with type 2 diabetes mellitus using a purposive sampling technique of 30 samples. Data collection was carried out by observing the medical record data of type 2 diabetes mellitus patients with Low-Density Lipoprotein (LDL) profile data for 2017. The results showed that patients with type 2 Diabetes Mellitus suffered the most by male sex with a percentage of 57%, aged over 45 years with a percentage of 97%, and having high LDL levels as much as 40%.
The total plate count of beras kencur herbal medicine in sub-district market Tulungagung city Eka Puspitasari; Chalies Diah Pratiwi; Tri Wahyuni
Medical Laboratory Analysis and Sciences Journal Vol 1 No 2 (2019): November 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v1i2.30

Abstract

Herbal medicine is a traditional drink made from natural ingredients for human health and is made with methods and equipment that are easier in its simplicity which often does not pay attention to hygiene factors and can be a medium for disease transmission. Based on the Regulation of the Head of the Republic of Indonesia Drug and Food Control Agency No. 12 of 2014 concerning requirements for drug fluids in the form of microbial contamination / ALT ≤104 colonies/ml. The purpose of this study was to study the estimated numbers of traditional herbs sold in the Tulungagung District market. The research design used is descriptive. The sampling technique uses a total sampling technique of 6 samples from 4 different markets. Sample meeting with the ALT method in May 2018 in the microbiology laboratory of STIKes Hutama Abdi Husada Tulungagung. The results of the study found that three out of six samples of traditional herbal medicine of kencur rice exceeded the microbial contamination threshold with an average yield of 1 x 107 CFU / ml. The study concludes is that 50% of the sample is not suitable for consumption and 50% of the sample is suitable for consumption. This requires Regulation of BPOM RI Number 12 the year 2014 Regarding Quality Requirements for Traditional Medicines, namely ≤104 colonies/ml. Herbal traders need to pay attention to raw materials, processing, equipment, air used, and containers used.
Antibacterial actifity of kecombrang (Etlingera elatior) leaf ethanol extract to against Klebsiella pneumoniae Eka Puspitasari; Chalies Diah Pratiwi; Dede Rival Novian; Dora Dayu Rahma Turista
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 7 No 01 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, IKIP Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i01.1663

Abstract

Infectious diseases caused by bacteria in generals can be cured using antibacterial. The use of antibacterials tends to cause local hypersensitivity either on the skin or mucous membranes so that their use is starting to be reduced. Therefore, the development of natural antibacterials made from plants to reduce resistance is very necessary. kecombrang (Etlingera elatior) is one of the plants that has been shown to have activity in inhibiting bacterial growth. This research is an experimental study that aims to determine the effectiveness of the ethanol extract of the leaves of kecombrang in inhibiting the growth of Klebsiella pneumoniae bacteria in vitro. The method used is the well method with a completely randomized design. The sample came from the Klebsiella pneumoniae bacteria culture. Kecombrang leaf ethanol extract was made in concentrations of 20%, 40%, 60%, 80% and 100%. The extract was tested on Klebsiella pneumoniae bacteria and observed for the formation of inhibition zones. Inhibition the results of the zone measuremens were then analyzed descriptively. The results showed kecombrang leaf ethanol extract 20%, 40%, 60%, 80%, and 100% produce inhibition zone diameter 1.9 mm; 2.6 mm; 7.7 mm; 12.1 mm; and 14.7 mm, 0.4 mm for the negative control and positive control 22.0 mm. Concentration 100% has better antibacterial ability due to higher compound content. The test results show the class of secondary metabolites kecombrang leaf ethanol extract contains flavonoids, tannin, and saponin. The conclusion of this study that the ethanol extract of kecombrang leaves has potential to inhibit the growth of Klebsiella pneumoniae bacteria.
Analysis of cholesterol and triglyceride levels in obese patients Chalies Diah Pratiwi; Eka Puspitasari; Andyanita Hanif Hermawati
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 8 No 01 (2023): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, IKIP Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v8i01.2518

Abstract

Obesity is excessive fat accumulation in the body. Continuous obesity and excessive food intake can cause metabolic system disorders in the form of hypercholesterolemia. Analysis of cholesterol and triglyceride levels in obese patients needs to be carried out to assist them in making healthier lifestyle changes. This study aims to analyze cholesterol and triglyceride levels in obese patients. The research method used is experimental with a laboratory approach. The samples to be analyzed were 20 obese people obtained by purposive sampling technique in the Tulungagung area. The research instruments used were height and weight check sheets and cholesterol and triglyceride test results sheets. Research data analysis technique by examining cholesterol and triglyceride levels using a MicroLab C-300 spectrophotometer and will be analyzed descriptively quantitatively. The results showed that 15 patients had normal cholesterol levels (75%) with criteria <200 mg/dl and 5 patients had high cholesterol levels (25%) with criteria >200 mg/dl, while there were 17 patients with normal triglyceride levels (85 %) with criteria <150 mg/dl and 3 patients had high triglyceride levels (15%) with criteria >150 mg/dl. The conclusion of this study is that people who are obese do not necessarily have high cholesterol and triglyceride levels. However, when a person weighs > 80 kg, it is recommended to start paying attention to a healthier lifestyle.
Co-Authors Andyanita Hanif Hermawati Chalies Diah Pratiwi Chalies Diah Pratiwi Chalies Diah Pratiwi Cholila Lailatul Nurmala Deny Yusnia Milasari Fanny Kurnanda Razvi Novian, Dede Rival Tri Wahyuni Turista, Dora Dayu Rahma Vivi Nurohmah