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All Journal Klinikal Sains (Jurnal Analis Kesehatan) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medical Laboratory Analysis and Sciences Journal Borneo Journal of Medical Laboratory Technology Journal of Community Service and Empowerment

Eka Puspitasari

STIKes Hutama Abdi Husada Tulungagung

Author-ID : 3389762

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Social Sciences Other

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Articles

Identifikasi Sedimen Urine pada Penduduk yang Mengkonsumsi Air Sumur di Desa Besole Kecamatan Besuki Kabupaten Tulungagung Chalies Diah Pratiwi; Eka Puspitasari
Borneo Journal of Medical Laboratory Technology Vol 1 No 2 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Drinking water that is consumed should be clean and healthy. To avoid illness and disruption of bodily functions such as kidney function, liver, brain, teeth, and even mental disorders. Some people in Besole village still use dug well water as a source of drinking water. The geographical state of the town of Besole, Tulungagung district, is a mountainous area of marble containing lime which allows for mineral content, main calcium in the drinking water of residents. This study aims to determine the identification of urine sediments in residents who consume well water in Besole Village, Besuki District, Tulungagung Regency in 2018. This type of research is descriptive non-analytic, by determining the percentage of urine sediments that consume well water through an examination at the Pathology Laboratory STIKes Hutama Abdi Husada Tulungagung. The number of samples is 30 people, with simple random sampling technique. Based on the research conducted found the results of abnormal urine sediment percentage, ie, 6.7% leukocytes, 3.3% erythrocytes, 16.7% epithelium, 30% calcium oxalate and 3.3% bacteria. Water containing lime should be boiled, precipitated and filtered before consumption.

Deskripsi Jumlah Leukosit dan Laju Endap Darah pada Pasien Tuberkulosis di Rumah Sakit Paru Dungus Madiun Chalies Diah Pratiwi; Eka Puspitasari; Vivi Nurohmah
Borneo Journal of Medical Laboratory Technology Vol 2 No 1 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Tuberculosis is an infectious disease of global concern caused of bacterium Mycobacterium tuberculosis. It can attack lung, liver, kidneys, bones or skin. If a person infected by tuberculosis, the body will begin the mechanism of inflammation as a form of haematological manifestation. The purpose of this study was to describe the number of leukocytes and Blood Sedimentation Rate (BSR) in tuberculosis patients. The research design used is non-analytic descriptive. The population in this study were all tuberculosis patients at the Dungus Madiun Lung Hospital in 2018Using the Quota Sampling technique, the sample in this study was 30 respondents. This research was conducted on May 2018. The results of the study on tuberculosis patients showed that the number of leukocytes in TB patients was 9120 cells/µL blood, 11 (37%) patients had leukocytosis and 19 (63%) sufferers have a nominal leukocyte count. The lowest leukocyte count was 4100 cells/µL of blood and the highest was 24700 cells/µL of blood. The average value of BSR in TB patients was 71 mm/hour I, 29 (96.6%) patients experienced an increase in BSR values and 1 (3.4%) patients had normal BSR values. The lowest value of BSR is 11 mm/hour I and the highest value of BSR is 106 mm/hour I. Prevention of tuberculosis infection can be done by conducting laboratory diagnosis to find out how far the disease goes and choose the right OAT for patients. With intensive treatment, it will reduce the number of leukocytes and reduce the risk for MDR TB

Review: Perbedaan Kadar Hemoglobin Menggunakan Hematologi Analyzer dan Spektrofotometer pada Ibu Hamil Andyanita Hanif Hermawati; Eka Puspitasari; Deny Yusnia Milasari
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2388

Hemoglobin is the main component of red blood cells, a conjugated protein that functions to transport oxygen and carbon dioxide. Hemoglobin examination can be done using a hematology analyzer and spectrophotometry. This study aims to determine the data analysis whether there is a difference in hemoglobin levels using a hematology analyzer and spectrophotometry in pregnant women. The method used is Literature Review; the nature of this research is descriptive. The population of this research is health journals that have been through screening, namely journals of differences in hemoglobin levels using a hematology analyzer and spectrophotometry in pregnant women. In this study, the data used are secondary data and data analysis using qualitative data. The results of the evaluation of the study by the Literature Review with hemoglobin levels in pregnant women at Hermina Hospital, Surakarta, showed that the average hemoglobin level of 30 samples of pregnant women using a hematology analyzer was below normal, namely 10.3 gr/dL and normal hemoglobin level is 12.80 gr/dL. The hemoglobin level using spectrophotometry and hematology analyzer on the sample was immediately checked, the results of the hemoglobin levels were 12.98 gr/dL and 11.70 g/dL, respectively. The hemoglobin level showed that the average hemoglobin before being given Fe-Folate with orange water was 11.71 g/dL. The conclusion of this study is the difference in hemoglobin levels is not much different, so there is no difference between the spectrophotometry and the hematology analyzer

Low density lipoprotein (LDL) in type 2 diabetes mellitus Andyanita Hanif Hermawati; Eka Puspitasari; Cholila Lailatul Nurmala
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Hyperglycemia due to abnormal insulin secretion, insulin work or both. Type 2 Diabetes Mellitus is the most among other types of diabetes mellitus. Diabetes mellitus is usually accompanied by dyslipidemia. Dyslipidemia is a disorder of lipid metabolism or cholesterol characterized by an increase or decrease in lipid fraction in the plasma. Diabetes mellitus and elevated Low-Density Lipoprotein (LDL) cholesterol levels are often found to be interrelated. The purpose of this study was to describe Low-Density Lipoprotein (LDL) levels in patients with type 2 diabetes mellitus. The research design used was descriptive non-analytic. The population in this study were all patients with type 2 diabetes mellitus using a purposive sampling technique of 30 samples. Data collection was carried out by observing the medical record data of type 2 diabetes mellitus patients with Low-Density Lipoprotein (LDL) profile data for 2017. The results showed that patients with type 2 Diabetes Mellitus suffered the most by male sex with a percentage of 57%, aged over 45 years with a percentage of 97%, and having high LDL levels as much as 40%.

The total plate count of beras kencur herbal medicine in sub-district market Tulungagung city Eka Puspitasari; Chalies Diah Pratiwi; Tri Wahyuni
Medical Laboratory Analysis and Sciences Journal Vol 1 No 2 (2019): November 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v1i2.30

Herbal medicine is a traditional drink made from natural ingredients for human health and is made with methods and equipment that are easier in its simplicity which often does not pay attention to hygiene factors and can be a medium for disease transmission. Based on the Regulation of the Head of the Republic of Indonesia Drug and Food Control Agency No. 12 of 2014 concerning requirements for drug fluids in the form of microbial contamination / ALT ≤104 colonies/ml. The purpose of this study was to study the estimated numbers of traditional herbs sold in the Tulungagung District market. The research design used is descriptive. The sampling technique uses a total sampling technique of 6 samples from 4 different markets. Sample meeting with the ALT method in May 2018 in the microbiology laboratory of STIKes Hutama Abdi Husada Tulungagung. The results of the study found that three out of six samples of traditional herbal medicine of kencur rice exceeded the microbial contamination threshold with an average yield of 1 x 107 CFU / ml. The study concludes is that 50% of the sample is not suitable for consumption and 50% of the sample is suitable for consumption. This requires Regulation of BPOM RI Number 12 the year 2014 Regarding Quality Requirements for Traditional Medicines, namely ≤104 colonies/ml. Herbal traders need to pay attention to raw materials, processing, equipment, air used, and containers used.

Scabies education and detection at Raudlatul Musthofa junior high school Eka Puspitasari; Qurrotu A’yuunin Lathifah; H. Hariyanto; Yan Fu'ana
Journal of Community Service and Empowerment Vol. 4 No. 3 (2023): December
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v4i3.28243

Scabies is a skin disease endemic in tropical and subtropical climates that is highly contagious and generally affects groups of people with low socioeconomic conditions, poor personal hygiene and sanitation. This activity aimed to make efforts to prevent, provide education, and assist public health development so that it is expected to improve public health in boarding schools students. This study aims to make efforts to prevent, provide education, and assist public health development so that it is expected to improve public health in boarding schools. The sample to be analyzed was 19 students from Raudlatul Junior High School. Examination of parasites that cause scabies is done with a skin swab. The results of observations that have been made of Raudlatul Musthofa Junior High School students show that the majority of students have carried out personal hygine well, namely all 19 students (100%) bathing at least twice a day using soap, keeping hands and feet clean, and changing clothes when dirty. A total of 14 students (74%) do not exchange personal items such as towels, do not take turns in clothes, and do not dry clothes in the room. A total of 15 students (79%) cleaned the bed, 18 students (95%) did not lend toiletries such as soap, and 16 students (84%) did not experience symptoms of frequent itching at night, rashes (spots), wounds, or dry and crusty skin. The results of skin swab examinations on 19 students at Raudlatul Musthofa seen under a microscope obtained as many as 13 students (68%) samples contained bacteria and as many as 6 students (32%) samples contained fungi.

The EFFECT OF DURATION AND AMOUNT OF SMOKING ON THE GROWTH OF STAPHYLOCOCCUS AUREUS: PENGARUH LAMA DAN JUMLAH MEROKOK PADA PERTUMBUHAN STAPHYLOCOCCUS AUREUS EKA PUSPITASARI; Imelda Putri Kusharyati; Qurrotu A’yunin Lathifah
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 2 (2025): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v13i2.6804

Smoking is a problem that is difficult to stop, causing dangerous impacts for smokers. Various diseases caused by smoking can cause death for active smokers and passive smokers in mild to severe degrees, such as the emergence of systemic or local pathological conditions in the mouth or throat. This occurs due to a decrease function and damage to the antioxidant components of saliva, which is followed by a decrease in function. Staphylococcus aureus is an oral bacteria that is commensal but can be a pathogen in humans causing nosocomial Objective: Is there a relationship between the duration of smoking and the amount of cigarette consumption on the growth of Staphylococcus aureus bacteria in active smokers. Methods: analytical observational with a cross sectional design. The sampling technique used was non-probability sampling with a purposive sampling method Results: were positive for Staphylococcus aureus as many as 20 respondents. Data analysis carried out using the Chi-Square test obtained a p value < 0.01 and data analysis on the amount of smoking obtained a p value < 0.003. Conclusions: There is a significant relationship between the duration and amount of cigarette consumption on the growth of Staphylococcus aureus bacteria in active smokers.

The Potential Use of EDTA as an Alternative to Defibrination in Preparing Blood Agar Plates with Human AB Blood Type on Staphylococcus aureus Culture Dora Dayu Rahma Turista; Eka Puspitasari; Fanny Kurnanda Razvi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1923

Blood Agar Plates (BAP) are composed of blood as one of the compositions. Sheep’s blood is usually used, but since it is difficult to be obtained, human AB blood type was used as an alternative. In preparing BAP, blood is defibrinated to lyse the blood clotting factors. Blood clots can also be prevented by adding anticoagulants, such as ethylenediaminetetraacetic acid (EDTA). This study aims to investigate the potential use of EDTA as a substitute for defibrination in preparing BAP with human AB blood type. This study employed a completely randomized design with true experimental method using Staphylococcus aureus as the sample. The parameters were the number of colonies, types of hemolysis, and hemolysis zone. The results showed that the S. aureus grown on BAP with EDTA-human AB blood type was 64 colonies (mean), produced β-hemolytic pattern, and 6 mm hemolytic zone. In contrast, the S. aureus grown on BAP with defibrinated human AB blood type showed 82 colonies (mean), β-hemolytic pattern, and 5 mm hemolytic zone. There were significant differences in the number of colonies (0.000 < α) and hemolytic zones (0.02 < α). However, there was no difference in the hemolysis type (both treatments produced β-hemolysis). EDTA was possible to be used as a substitute for defibrination in preparing BAP to assess the hemolysis type of S. aureus, but it might not be able to be used as a benchmark for counting the number of colonies and determining the hemolysis zone of S. aureus.

Co-Authors Andyanita Hanif Hermawati Chalies Diah Pratiwi Chalies Diah Pratiwi Chalies Diah Pratiwi Cholila Lailatul Nurmala Deny Yusnia Milasari Fanny Kurnanda Razvi H. Hariyanto Imelda Putri Kusharyati Novian, Dede Rival Qurrotu A’yunin Lathifah Qurrotu A’yuunin Lathifah Tri Wahyuni Turista, Dora Dayu Rahma Vivi Nurohmah Yan Fu'ana

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