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Pankie N.L Pangemanan
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Environmental Science

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Kualitas air disekitar lokasi budi daya ikan di Desa Paslaten Kabupaten Minahasa Yahuli, Yason; Pangemanan, Pankie N.L; Rompas, Robert J
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.2.2014.4902

Abstract

The purpose of this study was to determine the current state of water quality in fish farming locations in the waters of Lake Tondano . Sampling points at each station are placed vertically in three predetermined points from the guard house to the front of the net.  The distance between one sampling  point to the next was ± 10 m. Water quality including physicals and chemicals parameters were analyzed at the Industrial Research Board of Manado. Sampling  point was made purposively as refers to the physiographic location in order to represent or describe these waters condition. This study was conducted for 2 weeks. Water sampling was done in three times, in the  morning, at noon, and in the evening. Direct measurement (in situ) was performed once a week that included DO, pH, temperature, and brightness, while the laboratory tests were conducted for 2 weeks which included phosphate, turbidity, Nitrite, Nitrate. Research results  showed that temperature, DO, pH, brightnes, phosphate, turbidity, nitrite, nitrate are still in good condition based on water quality standard stated in PP No.82 of 2001 . Keywords: Lake Tondano, water quality, chemical Parameter, physical Parameter
Identifikasi bakteri Vibrio spp. penyebab vibriosis pada udang vanname (Litopenaeus vannamei) di PT. Pilar Persada Parigi Putra, Stevano F.P; Tumbol, Reiny A.; Undap, Suzanne L.; Kreckhoff, Reni L.; Pangemanan, Pankie N.L
e-Journal BUDIDAYA PERAIRAN Vol. 12 No. 2 (2024): Juli - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v12i2.58872

Abstract

The purpose of this study is to isolate and identify Vibrio spp. bacteria from vannamei shrimp (Litopenaeus vannamei) in PT. Pilar Persada Parigi Bajo Village, South Minahasa Regency. The research was carried out by starting with shrimp sampling from shrimp cultivation locations at PT. Persada Parigi Pillar in Bajo Village, Tatapaan, South Minahasa, North Sulawesi as many as 9 fish from 3 ponds (1 pond took 3 shrimp). The pond was recommended by the pond technician of PT. The Persada Parigi pillar is related to the condition of the pond which is suspected of having Vibrio spp. bacteria that need special attention. The shrimp samples were then taken to the Laboratory alive. During transportation, the samples were put in 3 separate containers equipped with battery aerators, then the identification of Vibrio spp. bacteria was carried out at the Laboratory of Pharmaceutical Microbiology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University and at the Manado Fishery Products Quality Control and Safety Quarantine Center. Isolation of Vibrio spp. bacteria using TCBS (Thiosulfate Citrate Bile Sucrose) agar media. The characterization of Vibrio spp. bacteria was carried out by a series of tests, namely gram staining tests (gram negative, positive and bacterial form), main tests (KOH 3%, H₂O₂ and oxidase), Oxidation/Fermentation (O/F), Glucose and Motility Indole Ornithin (MIO). The results of the isolation showed that there were 9 isolates of Vibrio spp. bacteria from 9 samples of vannamei shrimp (Litopenaeus vannamei) from 3 different ponds. The results of bacterial characteristics obtained negative grams, yellow and green colony morphology, bacil/stem cell shape, KOH 3% (-), catalase (+), oxidase (+), O/F (fermentative), indole (+), and motility (+), glucose (+) from the results identified by Vibrio spp. bacteria. Keywords: Litopenaeus vannamei, Vibrio spp., Vibriosis, isolation, identification  
Studi kandungan senyawa tributiltin dalam air pada area budidaya di Selat Lembeh Bitung Simanjorang, Daniel; Undap, Suzanne L.; Tumembouw, Sipriana S.; Pangemanan, Pankie N.L; Pangkey, Henneke; Mudeng, Joppy D.
e-Journal BUDIDAYA PERAIRAN Vol. 13 No. 1 (2025): Jan - Juni
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v13i1.59059

Abstract

Tributiltin (TBT) is an organotin compound that is still widely used as a toxic biocidal additive in antifouling paints. These compounds are classified as pollutants that are very difficult to decompose in waters. The test of the concentration of TBT in seawater was carried out at the WLN Laboratory (Water Laboratory Nusantara) Manado. Nitrate, nitrite and ammonia concentration tests were carried out at the Laboratory of the Manado Industrial Service and Standardization Center (BSPJI). Measurement of the physical and chemical parameters of seawater used: Temperature, Brightness, pH, Dissolved Oxygen (DO), Salinity and Turbidity in situ. Analysis of the concentration of Tributiltin (TBT) compounds in seawater in the waters of the Lembeh Strait in the cultivation areas of Point 1 (Tanjung Rarandam), Point 2 (Kalapa Dua), Point 3 (Papusungan) in the Lembeh Strait showed a concentration of <0.01 μg/L, which is still in the safe range below the standard limit of seawater quality for biota (0.01 μg/L). Measuring water quality in the Lembeh Strait aquaculture area, the DO value ranges from 7.83-8.34 ppm. Salinity values ranged from 30.99-31.24 ppt. The pH value ranges from 9.37-9.42. The temperature value ranges from 27.87-27.69 °C. The brightness value ranges from 3.12 m-3.39 m. The turbidity value ranged from 0.63-1.22. The nitrate value of 1.98-2.19 mg/L, far exceeding the limit set (0.06 mg/L) for marine life, indicates a potential risk to the ecosystem. The concentration of Nitrite in the waters is still in accordance with the general water standards, ranging from <0.005 mg/L to 0.02 mg/L. The concentration of Ammonia is below the water quality standard threshold at all points of 0.3 mg/L, which is still safe for marine life and aquaculture activities in these waters in accordance with the seawater quality standards in Government Regulation No. 22 of 2021 Attachment VIII concerning the Implementation of Environmental Protection and Management. Keywords: TBT Compounds, Concentration, Water Quality, Quality Standards, Straits
Co-Authors Henneke Pangkey Joppy Mudeng Kreckhoff, Reni L. Putra, Stevano F.P Reiny A. Tumbol Robert J Rompas Simanjorang, Daniel Sipriana S. Tumembouw Suzanne L Undap Yason Yahuli