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All Journal AKULTURASI (JURNAL ILMIAH AGROBISNIS PERIKANAN) AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX JURNAL PERIKANAN DAN KELAUTAN TROPIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Jurnal Akuatika Indonesia Media Akuakultur
Henneke Pangkey
Sam Ratulangi University, Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering

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EVALUASI KOMBINASI PAKAN DAN ESTRADIOL_17β TERHADAP PEMATANGAN GONAD DAN KUALITAS TELUR IKAN LELE DUMBO (Clarias gariepinus) Sinjal, Hengky; Ibo, Frengky; Pangkey, Henneke
JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Vol 1, No 1 (2014)
Publisher : JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi jenis pakan dan hormon estradiol_17β terhadap lama waktu matang, fertilisasi, daya tetas dan sintasan larva ikan lele dumbo (Clarias gariepinus). Metode yang digunakan pada penelitian ini adalah model eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 2 faktor perlakuan yang dikombinasikan, dimana faktor pakan terdiri dari 3 jenis pakan yaitu pakan Profish, pakan KRA dan Pakan Profish + pakan KRA. Sedangkan faktor hormon estradiol_17β terdiri dari 3 dosis hormon estradiol_17β yaitu dosis hormon estradiol_17β 0, 250 dan 500 μg/kg berat tubuh ikan, sehingga terdapat 9 kombinasi perlakuan dan masing masing perlakuan diulang 3 kali. Jumlah keseluruhan satuan percobaan adalah 27 satuan percobaan. Penelitian ini dilaksanakan di Balai Budidaya Air Tawar Tatelu, Penelitian ini menggunakan induk lele dumbo betina dengan berat 600 – 700 g/kg sebanyak 27 ekor. Penyuntikan hormon estradiol_17β pada induk ikan dengan perlakuan perbedaan dosis hormon estradiol_17β sesuai dengan disis yang telah ditentukan. induk ikan dipelihara dalam 9 bak beton berukuran 3 x 1,5 x 1,5 meter , masing-masing bak diisi 3 ekor ikan dan diberi makan sesuai dengan jenis pakan perlakuan. Setiap 2 minggu dicek kematangan gonadnya. Lama waktu matang dihitung dari penyuntikan sampai ikan matang gonad. Setelah ikan matang gonad dilakukan pemijahan buatan dengan menyuntikan ovaprim, kemudian induk ikan lele distripping telurnya dan dicampur dengan sperma yang telah disiapkan dari ikan jantan. Selanjutnya pembuahan diamati setelah 6 jam setelah sperma dan telur dicampur. Penetasan dilakukan dengan mengambil 200 telur yang telah berbuah dan di inkubasi selama 2 hari. Telur yang menetas dihitung. Larva hasil penetasan diambil 100 ekor dari masing masing perlakuan. Larva tersebut dipelihara di loyang selama 14 hari dan diberi makan. Larva yang mati dihitung. Data hasil penelitian dianalisis dengan JMP (SAS Institute). Data yang dianalisis adalah data lama waktu matang gonad (hari), pembuahan telur (%) , daya tetas telur (%), dan sintasan hidup larva. Dari hasil yang diperoleh adalah : Perlakuan yang terbaik adalah pemberian jenis pakan KRA dan penyuntikan hormon estradiol_17β dengan dosis 250 μg/kg berat tubuh ikan dengan hasil lama waktu matang gonad 21,67 hari, pembuahan telur 92%, daya tetas telur 87,33% dan sintasan larva 91,67%. Dari penelitian ini disarankan perlu dilakukan penelitian lanjutan dengan melihat hal yang lebih spesifik dan menganalisis mengenai kebutuhan asam amino serta asam lemak esensial serta kadar estradiol_17β dalam darah bagi suksesnya reproduksi ikan lele dumbo.Kata Kunci : Kualitas telur, Clarias gariepinus, Profish, pakan KRA, estradiol_17β
Pemanfaatan tepung kulit pisang raja (Musa paradisiaca) dalam formulasi pakan ikan nila (Oreochromis niloticus) Nanariain, Rian M.; Lumenta, Cyska; Pangkey, Henneke
e-Journal BUDIDAYA PERAIRAN Vol 5, No 1 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.5.1.2017.14866

Abstract

The objective of this research was to evaluate the effect of supplementation of banana skin (Musa paradisiaca) in feed formulation on absolute growth, relative growth, daily growth and feed efficiency of nile tilapia.  The research was conducted at the Laboratory of Aquaculture Technology, Faculty of Fisheries and Marine Science, Sam Ratulangi University. The research used Complete Randomized Design with five treatments inluded A: without supplementation banana skin powder, B: 40%, C: 30%, D: 20% and E: 10% of banana skin powder. Each treatment  had three replications.  The result showed the best response was observed in treatment D  with absolute growth o43.11 g, relative growth 88.9%, daily growth 10.17%,  and feed efficiency 3.20%. Keywords:  feed formulation, banana skin, nile tilapia, absolute growth, relative growth,   daily growth,  feed efficiency
Budidaya Maggot (Hermetia illuens) dengan menggunakan beberapa media Mudeng, Nico E.G.; Mokolensang, Jeffrie F.; Kalesaran, Ockstan J.; Pangkey, Henneke; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 6, No 3 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.3.2018.21543

Abstract

The purpose of this study was to determine the best culture medium for producing maggot (Hermetia illucens) and to determine the production quantity in each different medium.  This research was controlled two times a day at 09:00 a.m. and 16:00 p.m. Cultivation media were prepared from coconut pulp, tofu pulp, rice bran and restaurant wastes. Each medium was weighed as much as one kg and then placed into a culture container. The research used Completely Randomized Design consisting of 4 treatments, each with three replications. The parameters observed consisted of temperature and pH of the media  measured at 07.00 am and 17.00 pm, and maggot production measured on the last day. During the ten days of experiment, the production of maggot in the treatment using restaurant waste media was significantly different as compared to other treatments. This study found that a good medium for the production of maggot was prepared using restaurant waste. Keywords: Hermetia illucens, coconut pulp, restaurant waste
Pertumbuhan Cladocera jenis Chydoridae pada media kultur yang berbeda Sambode, Denovis; Pangkey, Henneke; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 1, No 2 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.2.2013.1910

Abstract

The aim of this study was to find out the effect of different organic fertilizers on the population growth of Chydorus sp. This research was conducted at Laboratorium of Nutrition and Food Technology, Faculty of Fishery and Marine Sciences, Sam Ratulangi University, from December, 2012 to January, 2013. The experiment was run in the Completely Randomized Design with 3 treatments and 3 replication. Chydorus sp. was cultured in 9 glass jars with a volume of 1 L each. Three treatments applied in this research included A: medium culture composed of 500ml of water and 50 grams of soil; B: medium culture composed of 500ml of water, 50 grams of soil and 10g of cow dung; C: medium culture composed of 500ml of water, 50 grams of soil and 10g of horse dung. The density of Cydorus sp. in each media was 10 individuals /500 ml water. Observation on the population growth, development of live preys, and water quality parameters was conducted for 20 days period. Data were statistically analized with Analysis of variance. The results showed the highest density of Chydorus sp. was reached by Chydorus cultured in medium with horse manure (2169 individual/500 ml), followed by medium culture with cow manure (1715 individual/500 ml), and the lowest in medium culture with soil which was 1065,33 individual/500 ml. However, Analysis of variances showed that Chydorus population growth were not significantly affected by different culture medium. Keywords: manure, liquid fertilizer, growth, Chydorus sp.
Pengaruh penambahan karbon aktif terhadap pertumbuhan dan kelangsungan hidup lele (Clarias gariepinus) dalam wadah terkontrol Tamba, Ermaulina; Mokolensang, Jeffrie F.; Pangkey, Henneke; Longdong, Sammy N.J.; Undap, Suzanne L.
e-Journal BUDIDAYA PERAIRAN Vol 7, No 1 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.7.1.2019.24389

Abstract

This study aimed to determine the effect of addition of different activated carbon on growth of catfish, feeding efficiency, and feeding conversion ratio and survival rate. This research was carried out in the Aquaculture Technology laboratory, Faculty of Fisheries and Marine Science. The media test was an aquarium with the size of 60 x 40 x 40 cm placed in laboratory. The experimental design was a completely randomized design with 5 treatments and 3 repetitions. The charcoal used in this experiment was A: 1%, B: 2%, C: 3%, D: 4%, E: without charcoal. The observed variables were total growth, relative growth, daily growth, feeding efficiency, feed conversion ratio, and survival rate. The results of statistical analysis on growth parameters indicated the same effect on the growth of 4-6 cm catfish juvenile. The highest weight gain was achieved in treatment C (2,62±0,1), which was pellet feeding with a composition of 3% charcoal, as well as relative growth (413,47±36,3), and daily growth (1.3±0,2). The treatment showed significant effect on efficiency. The highest feed conversion ratio was in treatment E (2,06±1,4), which was pellet feeding with 0% charcoal, and the lowest in treatment C (0,76±0,1). This study concluded that addition of charcoal 1%, 2%, 3%, 4% and without charcoal had the same effect in triggering 4-6 cm catfish growth.Keywords: Active carbon, catfish, growth, feeding efficiency, feeding conversion
Pengaruh beberapa jenis pakan hijauan terhadap pertumbuhan ikan Koan Stenopharyngodon idella Babo, Desmianti; Sampekalo, Julius; Pangkey, Henneke
e-Journal BUDIDAYA PERAIRAN Vol 1, No 3 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.3.2013.2716

Abstract

The aim of this research was to determine effect of feed plants on growth of grass carp, Stenopharyngodon idella. Grass carp used had an average length of 12.8 cm and weight of 21.00 g. Fish were kept in 12 pouch nets with a density of 8 fish/net. Fish was fed in et libithum, twice a day. This research used Completely Randomized Design with four treatments: A Pennistum purpureum, B Eichornia crassipes, C Pistia stratiotes and D Azolla pinnata, each with three replications. Data from each treatment were statistically analyzed using analysis of variance and LSD. Research results showed that growth of fish in treatment C was significantly different as compared to other treatments. Length of fish reached 3.30 cm, absolute growth 22.12 g and relative growth achieved 103.60%. thus, application of feed plant Pistia stratiotes might improved growth of grass carp.
Pengaruh penambahan karbon aktif terhadap parameter pertumbuhan dan kelangsungan hidup ikan nila (oreochromis niloticus) dalam wadah terkontrol Iroth, Revelino A.; Mokolensang, Jeffrie F.; Pangemanan, Novie P.L.; Lantu, Sartje; Pangkey, Henneke; Sondakh, Calvyn F.A.
e-Journal BUDIDAYA PERAIRAN Vol 7, No 2 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.7.2.2019.26117

Abstract

The Aim of this study was to determine the effect of additional activated carbon in fish feed with different composition for the growth performance of Nile (Oreochromis niloticus), include feed efficiency (FE), specific growth rate (SGR) and feed conversion ration (FCR). This research was carried out indoor Laboratory. The test container used was an aquarium measuring 60 x 40 x 40 cm placed in a laboratory. The experimental research design method used was a completely randomized design (ANOVA) with 5 treatments and 3 replicates. The treatments used were A: Control, B: 1%, C: 2%, D: 3%, E: 4%. The results of statistical analysis on the growth variables showed the same effect on the growth of tilapia. The best weigth gain on growth performance occurred in treatment D (4.49), fish feeding with pellet charcoal composition of 3%, as well as relative growth (101.4), and daily growth (2.32%). The results of the statistical analysis of the effect of treatment on the value of efficiency provide a very real effect. The highest value of feed conversion occurred in treatment C (1.05), namely the feeding of pellets with charcoal composition of 2%, and the lowest treatment B (1.86). This study can be concluded that the administration of 1%, 2%, 3%, 4% charcoal and without charcoal is the same effect in triggering the growth of 3-5 cm tilapia.
Pemanfaatan kotoran ternak dengan dosis yang berbeda terhadap pertumbuhan dan biomassa cacing sutra (Tubifex sp.) Wenda, Detiben; Pangkey, Henneke; Mokolensang, Jeffrie F. F.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20496

Abstract

The purpose of this study was to analyze the use of cattle dung on the growth and biomass of silk worms. The experiment was conducted at Freshwater Aquaculture Center (BPBAT) Tatelu, Tatelu Village, Dimembe Subdistrict, North Minahasa Regency, North Sulawesi Province, about 35 km from Manado city. The media used was pig manure, chicken manure, cow dung, and fine mud added with EM4. The method used was complete randomized design (RAL) with 4 treatments including A. 500 g of pig manure, 500 g of chicken manure 500 g of cow dung, and 500 g of fine mud; B. 600 g of pig manure, 400 g of chicken manure 500 g of cow dung, 500 g of fine mud; C. 700 g of pig manure, 300 g of chicken manure, 500 g of cow dung, 500 g of fine mud; and K (control) was 2000 g of fine mudinand, each with 3 replications. Water quality parameters measured during the study were temperature, pH, DO, nitrate and nitrite. The results showed that there was a very significant effect on growth but not for the value of silk worm biomass. The highest growth was found in treatment A that was 38 g, while for the highest biomass also in treatment A namely 1.5 g / cm3. Water quality parameters during the study were 24.3-25.4° C, pH 7,1-7,3; DO 2.7-5,7 ppm; nitrate 1.1-1.4 ppm; nitrite 0.011-0.201 ppm.Keywords:  Cattle dung, growth, biomass, Tubifex sp., aquaculture
Ratio of C:N in culture media of silk worm, Tubifex sp. Solang, Jhonly; Pangkey, Henneke; Wullur, Stenly; Lantu, Sartje
AQUATIC SCIENCE & MANAGEMENT Vol 2, No 1 (2014): April
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.2.1.2014.12391

Abstract

Title (Bahasa Indonesia): Rasio C:N pada media kultur cacing sutra, Tubifex sp. This study aimed to determine the C:N ratio on each medium for the growth of the sludge worm. This study used mud and soybean curd residue (treatment A), mud and chicken manure (treatment B), mud and pig manure (treatment C), and control (mud only) (K) as culture media of the sludge worm (Tubifex sp.). The decomposition process was proceeding for six days, and then the sludge worms were stocked with initial weight of 30 grams per container. Culture period was 21 days in running water systems. The resulting C:N ratio was 60.5 for treatment A, 45.8 for treatment B, 36 for treatment C and 35 for K. The soybean curd residue and mud medium gave the highest influence on the growth of the sludge worm, followed by pig manure and mud, chicken manure and mud, and then mud (control). Penelitian ini bertujuan untuk menentukan perbandingan C:N ratio dalam media budidaya untuk pertumbuhan cacing sutra. Penelitian ini menggunakan lumpur dan ampas tahu (perlakuan A), lumpur dan kotoran ayam (perlakuan B), serta lumpur dan kotoran babi (perlakuan C) dan perlakuan K (kontrol: hanya lumpur) sebagai media kultur cacing sutra (Tubifex sp). Proses dekomposisi dilakukan selama 6 hari, kemudian dilakukan penebaran cacing sutra dengan berat awal 30 gram/wadah penelitian. Waktu pemeliharaan dilakukan selama 21 hari dalam sistem air mengalir. Hasil penelitian menunjukkan rasio C:N sebesar 60,55 untuk perlakuan A, 45,85 untuk perlakuan B, 36,08 untuk perlakuan C, dan 35,25 untuk K. Media yang menggunakan ampas tahu dan lumpur memberikan pengaruh tertinggi terhadap pertumbuhan cacing sutra, kemudian disusul oleh media kotoran babi dan lumpur, media kotoran ayam dan lumpur dan terendah media lumpur (kontrol).
Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida) Letsoin, Petrus P; Pangkey, Henneke; Sampekalo, Julius; Rumengan, Inneke F.M; Wullur, Stenly; Rimper, Joice R.S.T.L
AQUATIC SCIENCE & MANAGEMENT Vol 1, No 2 (2013): Oktober
Publisher : Graduate Program of Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.1.2.2013.7276

Abstract

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae.  Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.
Co-Authors Adnan Wantasen Alex D. Kambey Ali Djamhuri Arne A Ratulangi Calvyn F. A. Sondak, Calvyn F. A. Cyska Lumenta Deiske Adeliene Sumilat, Deiske Adeliene Denovis Sambode Desmianti Babo Durand, Swenekhe S. Edwin D Ngangi Erly Kaligis Fanny Silooy Fintarji, Rian Frengky Ibo Gerung, Pramulya R.A Ginting, Elvie L. Grace O. Tambani Hengky J Sinjal Hengky J. Sinjal, Hengky J. Hengky Manoppo Hengky Sinjal Henky Manoppo Hety B Lahope Indra R. N. Salindeho Indra R.N. Salindeho Indra RN Salindeho Inneke F. M Rumengan Iroth, Revelino A. Jhonly Solang Joice R.T.S.L Rimper Joppy Mudeng Joula Kusen, Diane Juliaan Ch. Watung Julius Sampekalo Kalesaran, Ockstan Jurike Kreckhoff, Reni L. Kreckhoff, Reni Lucia Kurniati Kemer Kusen, Diane Joula Kusuma, Ni Putu Dian Lexy K. Rarung Mantiri, Rose Markus T. Lasut Mingkit, Winda M. Mokolensang, Jeffrie F. Mokolensang, Jeffrie F. F. Mokolensang, Jeffrie Fredrik Monijung, Revol D. Mudeng, Nico E.G. Nanariain, Rian M. Natalie D Rumampuk Ngangi, Edwin Leonardo Apolonio Ngatung, Jefrikardus E. E. Niti, Eukharistian Nowin, Edgar Ockstan J. Kalesaran Pamula, Doni F. Pangandaheng, Agreystin Pangemanan, Novie P.L. Pangemanan, Novie Pankie Lukas Pangemanan1, Novie P. L. Pankie N.L Pangemanan Paulus, James Petrus P Letsoin Reiny A. Tumbol Reiny Tumbol Sambali, Hariyani Sambali, Haryani Sambali, M.Sc, Hariyani Sammy N.J. Longdong Sanggaria, Ayu W. Saragih, Debby Dyanessa Sartje Lantu Simangunsong, Martua Pinondang Simanjorang, Daniel Simanjuntak, Erika E. L. Sinjal, Hengky Sinjal Sipriana S. Tumembouw Soputan, Stevaldo J.M. Stenly Wullur Sumah, Maria Suzanne L Undap Tamba, Ermaulina Tununu, Riky R. Undi, Chintia S. Utami, Tiara Veibe Warouw Victoria E.N. Manoppo, Victoria E.N. Wenda, Detiben Winda M. Mingkid, Winda M. Wulur, Stenly