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Evaluating the yield potential of the mutant (M6) short stem Mentik Wangi rice varieties developed through 200-gray gamma irradiation Himawan, Rifqi; Yunus, Ahmad; Parjanto; Purwanto, Edi
Journal of Agrosociology and Sustainability Vol. 2 No. 2: (January) 2025
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/jassu.v2i2.2025.1463

Abstract

Background: Mentik Wangi, a traditional aromatic rice variety, faces challenges such as prolonged growth duration and lodging susceptibility, limiting its productivity. The study aimed to evaluate the yield potential and identify high-performing mutant lines of Mentik Wangi rice induced by 200 Gy gamma irradiation. This study addresses the growing need for rice varieties with improved traits to enhance food security in Indonesia. Methods: The research was conducted at the Tegalgondo Rice Seed Garden using a randomized complete block design (RCBD) with three replications. The study included 12 M6 mutant lines of Mentik Wangi rice generated through 200 Gy gamma irradiation. Data were collected on plant growth, yield attributes, and grain quality. Statistical analyses were performed using ANOVA and Duncan's Multiple Range Test to evaluate the significance of observed traits. Findings: The results revealed significant variations among mutant lines for key yield components, including grains per panicle, 100-grain weight, and productivity per hectare. The line M6-MW2-G70-01-14-4-8 demonstrated the highest productivity at 7.29 tons/ha, while all mutant lines exceeded the productivity of the control (3.78 tons/ha). Gamma irradiation was effective in inducing beneficial mutations, enhancing traits such as early maturity, short stems, and higher grain density. Conclusion: The study successfully identified mutant lines of Mentik Wangi rice with improved yield potential and agronomic traits, demonstrating the effectiveness of gamma irradiation as a crop improvement strategy. Novelty/Originality of this article: This research presents innovative findings on the use of gamma irradiation to enhance the productivity and agronomic traits of a traditional rice variety, contributing to the development of high-yielding and locally adapted rice lines.
Colchicine-Mediated Polyploidization For Improvement Of French Marigold Traits Aziz Nur Arifin; Parjanto; Endang Yuniastuti
International Journal of Interdisciplinary Research Vol. 2 No. 2 (2026): Vol 2 no 2 July 2026
Publisher : Ponpes As-Salafiyyah Asy-Syafi'iyyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71305/ijir.v2i2.1629

Abstract

This study evaluated the effects of colchicine concentration and soaking duration on vegetative morphology of French marigold (Tagetes patula L.) cv. Janie Spry, a naturally allotetraploid species (2n = 4x = 48). A 4×3 factorial completely randomized design was employed with four colchicine concentrations (0%, 0.1%, 0.2%, 0.3%) and three soaking durations (4, 8, 12 hours), totaling 12 treatments with three replications (n = 36). Germinated seeds at the radicle stage were soaked in colchicine solutions and grown in a greenhouse. Survival rate, plant height, stem diameter, and number of shoots were recorded during the vegetative phase. Two-way ANOVA revealed highly significant interaction effects (p<0.001) of concentration and duration on survival rate and shoot number, while plant height and stem diameter were not significantly affected. Survival declined sharply at higher concentrations with prolonged soaking, reaching only 30% at 0.3% colchicine for 12 hours. The 0.2% colchicine at 4-hour soaking treatment was identified as optimal, maintaining 90% survival while increasing shoot number by 37.7% relative to the control (30.7 vs. 22.3 shoots plant⁻¹). The highest shoot count (34.3 shoots plant⁻¹) occurred at 0.3% for 12 hours but was accompanied by unacceptable seedling mortality. These results indicate that moderate colchicine concentrations with short exposure offer an effective balance between morphological modification and seedling viability in T. patula. Cytological confirmation and floral trait evaluation will be reported in a subsequent publication.