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The activity of ethanol extract and aquadest of Jati Belanda (Guazuma ulmifolia Lamk) leaves against Candida albicans Imelda Putri Kusharyati; Muhammad Evy Prastiyanto; Wildiani Wilson; Nala Fidarotul Ulya
Medical Laboratory Analysis and Sciences Journal Vol 2 No 2 (2020): November 2020
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v2i2.57

Abstract

Fungi are the cause of infection, the development of fungal infections in Indonesia is influenced by environmental conditions so that microbes grow quickly and can increase significantly. Candida Albicans is a fungal disease that infects humans. As a country with a tropical climate, Indonesia has potential biodiversity as a medicine, one of which is Dutch teak (Guazuma ulmifolia Lamk) which has secondary metabolites that kill microbes that can be used as natural antifungals. The purpose of this study was to determine the antifungal activity of ethanol extract and distilled water of Dutch teak leaves on the growth of Candida Albicans. The method used is maceration with ethanol and distilled water solvents. The anti-fungal activity was evaluated by the good diffusion method with SDA media. The results of the antifungal activity of ethanol extract and distilled water of Dutch teak leaves against Candida Albicans were indicated by the formation of an inhibition zone around the wells on Candida Albicans ethanol extract concentrations of 25 mg / mL to 1000 mg / mL 8.6 mm - 15.6 mm, the distilled water extract has not formed a zone of inhibition at all concentrations. The conclusion is that the ethanol extract of Dutch teak leaves can inhibit the growth of Candida Albicans. while the distilled water extract was unable to inhibit the growth of Candida Albicans.
ANTIBACTERIAL ACTIVITY OF PARIJOTO FRUIT EXTRACT (Medinilla speciosa) ON BACTERIA EXTENDED SPECTRUM BETALACTAMASE (ESBL) ESCHERICHIA COLI AND METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) Rizka Ayu Wahyuni; Inesya Yuliana Putri; Eden Lambang Jayadi; Muhammad Evy Prastiyanto
Jurnal Media Analis Kesehatan Vol 10, No 2 (2019): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1262.478 KB) | DOI: 10.32382/mak.v10i2.1250

Abstract

Infection is one of the causes of death and pain that occurs mainly in Indonesia. Infections can be caused by Gram-negative and gram-positive bacteria such as the Extended Spectrum Beta Laktamase, Escherichia coli (E. coli) and Methicilin Resistant Staphylococcus aureus (MRSA), which are one of the Strain of S. aureus. The study aims to determine the antibacterial activity of Aquadest extracts, Methanol, Ethyl acetat and N-hexane of Parijoto (M. speciosa), MIC and MBC values against ESBL E. coli and MRSA bacteria. The samples in this study were Parijoto fruit extracts concentration 100 mg/mL. In this research antibacterial activity using the method of diffuse with the MHA media, while to know the value of MIC using methods dilution with MHB and MBC media using BAP media. The results were characterized by the formation of the largest barrier zone on the 11.6 mm methanol extract, the smallest MIC yield on N-hexane and aquadest extracts with a value of 50 mg/mL, whereas on MBC tests each extract resulted in a value of 50 mg/mL and While the hate zone against the MRSA bacteria is obtained the largest diameter of the hate zone in the N-hexane solvent, which is 23.17 mm in average, the largest MIC value in the aquadest solvent at a concentration of 50 mg/ml, and the largest MBC value in methanol solvent Concentrations of 50 mg/ml. So that it can be concluded Parijoto fruit extract has the potential as an alternative substitute for chemical antibiotics against MRSA bacteria whereas for bacteria ESBL E. coli has no potential as an alternative to antibiotic substitute.Keywords : Antibacterial, Escherichia coli, Methicilin Resistant Staphylococcus aureus, Medinilla speciosa
KEANEKARAGAMAN DAN POLA RESISTENSI BAKTERI PADA PASIEN YANG TERDIAGNOSA SEPSIS Murni Batara; Sri Darmawati; Muhammad Evy Prastiyanto
Jurnal Labora Medika Vol 2, No 2 (2018): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.496 KB) | DOI: 10.26714/jlabmed.2.2.2018.1-5

Abstract

Sepsis atau Systemic Inflammatory Response Syndrome (SIRS) adalah kondisi klinis akut dan serius yang muncul akibat adanya infeksi bakteri di dalam darah. Penelitian ini bertujuan untuk mengetahui keanekaragaman bakteri dan pola resistensi terhadap antibiotik pada sampel darah pasien yang terdiagnosa sepsis  di Laboratorium Klinik Swasta di Semarang pada bulan Mei-Juni tahun 2018. Metode penelitian ini yaitu deskriptif. Hasil penelitian didapatkan sebanyak 42 sampel darah yang ditanam pada media Bact Alert, 12 sampel dinyatakan positif tumbuh. Dari ke 12 sampel tersebut didapatkan 6 jenis bakteri penyebab sepsis yaitu Staphylococcus haemolitycus (25%), Staphylococcus aureus (16,66), Staphylococcus hominis (16,66%), Staphylococcus epidermidis (16,66%), Staphylococcus saprophyticus (8,33%),  Pantoea sp (8,33%), dan Bordetella hinzii (8,33%). Bakteri gram positif resisten terhadap antibiotik Benzylpenicillin, Oxacillin, Penicyline, dan antibiotik betalaktam lainnya. Sedangkan bakteri Pantoea sp resisten terhadap antibiotik Pipericillin/Tazobactam dan Bordetella hinzii resisten terhadap antibiotik Pipericillin/Tazobactam dan Nitrofurantoin
Suspect TB di Sekitar Tempat Tinggal Penderita TB Paru di Sambiroto Semarang Sri Darmawati; Muhammad Evy Prastiyanto; Apriliya Listiyanti
Prosiding University Research Colloquium Proceeding of The 7th University Research Colloquium 2018: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.115 KB)

Abstract

Tuberkulosis (TB) paru adalah penyakit infeksi yang disebabkan olehbakteri Mycobacterium tuberculosis, dan suspect TB adalah orangyang dicurigai menderita TB. Orang yang tinggal di sekitarpenderita TB berpotensi menjadi suspect (tersangka) TB. Tujuanpenelitian untuk mengetahui gambaran suspect TB di lingkungansekitar tempat tinggal 3 penderita TB Paru di wilayah Sambirotokota Semarang. Jenis penelitian adalah penelitian deskriptif, sampelsebanyak 30 suspect dari 3 titik tempat tinggal penderita TB ParuPositif, dari setiap titik tempat tinggal penderita diambil 10 orangsuspect yang masuk dalam screening penjaringan dengan kriteriaresponden adalah anggota keluarga yang tinggal serumah, tetanggadekat dan sering berkomunikasi secara langsung, kemudiandilakukan pemeriksaan mikroskopis dari dahaknya yaitu dahaksewaktu, pagi, sewaktu dengan pewarnaan metode Ziehl Nellson.Hasil pemeriksaan menunjukkan jumlah suspect Basil Tahan Asamdengan hasil positif sejumlah 4 sampel (13%) dari total 30 sampel.
Penelitian Pendahuluan: Aktivitas Antibakteri Cold Argon Plasma Jet terhadap Multi Drug Resistance (MDR) Bacteria Muhammad Evy Prastiyanto; Gita Rossa Maulida; Nia Salsabila; Sri Darmawati; N Nasruddin
Prosiding University Research Colloquium Proceeding of The 10th University Research Colloquium 2019: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.888 KB)

Abstract

Multi drug resistance (MDR) bacteria telah menjadi masalah kesehatan global diseluruh dunia dikarenakan penggunaan antibiotik yang tidak rasional. Tujuan dari penelitian ini untuk mengevaluasi aktivitas antibakteri cold argon plasma jet terhadap Methichillin Resistant Staphylococcus aureus (MRSA), Carbapenem Resistant Pseudomonas aeruginosa (CRPA), dan Extended Spectrum Betalactamase (ESBL) Escherichia coli. Proses uji antibakteri dilakukan dengan alat cold argon plasma jet pada jarak 1 cm dengan variasi waktu 1, 2, 3, 4, dan 5 menit. Aktivitas antibakteri cold argon plasma jet menunjukkan zona hambat tertinggi pada menit ke 5 CRPA 6,5 mm, ESBL E.coli 6,5 mm, MRSA 4 mm. Kesimpulan: cold argon plasma jet memiliki potensial sebagai agen antibakteri.
Suspect TB di Sekitar Tempat Tinggal Penderita TB Paru di Sambiroto Semarang Sri Darmawati; Muhammad Evy Prastiyanto; Apriliya Listiyanti
Prosiding University Research Colloquium Proceeding of The 7th University Research Colloquium 2018: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tuberkulosis (TB) paru adalah penyakit infeksi yang disebabkan olehbakteri Mycobacterium tuberculosis, dan suspect TB adalah orangyang dicurigai menderita TB. Orang yang tinggal di sekitarpenderita TB berpotensi menjadi suspect (tersangka) TB. Tujuanpenelitian untuk mengetahui gambaran suspect TB di lingkungansekitar tempat tinggal 3 penderita TB Paru di wilayah Sambirotokota Semarang. Jenis penelitian adalah penelitian deskriptif, sampelsebanyak 30 suspect dari 3 titik tempat tinggal penderita TB ParuPositif, dari setiap titik tempat tinggal penderita diambil 10 orangsuspect yang masuk dalam screening penjaringan dengan kriteriaresponden adalah anggota keluarga yang tinggal serumah, tetanggadekat dan sering berkomunikasi secara langsung, kemudiandilakukan pemeriksaan mikroskopis dari dahaknya yaitu dahaksewaktu, pagi, sewaktu dengan pewarnaan metode Ziehl Nellson.Hasil pemeriksaan menunjukkan jumlah suspect Basil Tahan Asamdengan hasil positif sejumlah 4 sampel (13%) dari total 30 sampel.
Penelitian Pendahuluan: Aktivitas Antibakteri Cold Argon Plasma Jet terhadap Multi Drug Resistance (MDR) Bacteria Muhammad Evy Prastiyanto; Gita Rossa Maulida; Nia Salsabila; Sri Darmawati; N Nasruddin
Prosiding University Research Colloquium Proceeding of The 10th University Research Colloquium 2019: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Multi drug resistance (MDR) bacteria telah menjadi masalah kesehatan global diseluruh dunia dikarenakan penggunaan antibiotik yang tidak rasional. Tujuan dari penelitian ini untuk mengevaluasi aktivitas antibakteri cold argon plasma jet terhadap Methichillin Resistant Staphylococcus aureus (MRSA), Carbapenem Resistant Pseudomonas aeruginosa (CRPA), dan Extended Spectrum Betalactamase (ESBL) Escherichia coli. Proses uji antibakteri dilakukan dengan alat cold argon plasma jet pada jarak 1 cm dengan variasi waktu 1, 2, 3, 4, dan 5 menit. Aktivitas antibakteri cold argon plasma jet menunjukkan zona hambat tertinggi pada menit ke 5 CRPA 6,5 mm, ESBL E.coli 6,5 mm, MRSA 4 mm. Kesimpulan: cold argon plasma jet memiliki potensial sebagai agen antibakteri.
Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria Muhammad Evy Prastiyanto; Fandhi Adi Wardoyo; Wildiani Wilson; Sri Darmawati
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23600

Abstract

The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller–Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best antibacterial activity, calculated as the most widely inhibitory zone and the smallest MBC values. The ethanolic extract showed antibacterial activity against the all MDR bacterial test in the agar well diffusion assay (10-14.5 mm inhibition diameter). The MBC of water extract against ESβL + CR Pseudomonas aeruginosa showed the best antibacterial activity (12.5 mg/mL). The fruit of bilimbi was shown to be potentially developed as antibacterial agents, especially for MDR strains. Further in vivo research and discovery of action mode are needed to shed light on their antibacterial effects. This study can provide new information about the benefits of bilimbi as a source of natural antibacterial againts MDR-bacteria
EDUKASI BAHAYA TUBERKULOSIS PARU KEPADA MASYARAKAT RENTAN DALAM UPAYA ELIMINASI TB DI MALAYSIA Muhammad Evy Prastiyanto; Arya Iswara; Sri Darmawati; Ahmad Naqib
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 6 (2024): Desember
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i6.26772

Abstract

Abstrak:.. Kegiatan edukasi mengenai bahaya tuberkulosis paru (TB) kepada masyarakat rentan di Malaysia bertujuan untuk meningkatkan kesadaran dan pemahaman tentang penyakit ini serta langkah-langkah pencegahannya. Dengan meningkatnya angka kasus TB di Malaysia, yang mencapai 26.781 kasus pada tahun 2022, termasuk 2.623 kematian, kegiatan ini menjadi sangat penting dalam upaya eliminasi TB. Melalui pendekatan partisipatif, edukasi dilakukan di komunitas dengan fokus pada kelompok berisiko tinggi, seperti individu dengan kondisi kesehatan yang mendasari dan mereka yang tinggal di lingkungan padat penduduk. Metode yang digunakan meliputi penyuluhan, distribusi materi informasi, dan penggunaan masker yang benar untuk memastikan pemahaman yang baik tentang gejala, cara penularan, dan pentingnya penggunaan masker serta kepatuhan terhadap pengobatan yang dilakukan di Pusat kecemerlangan perkhidmatan klinik UCMI Malaysia. Pasien rentan terinfeksi oleh bakteri penyebab TB, yang merupakan pasien tetap dengan usia senja pada Pusat kecemerlangan perkhidmatan klinik hemodialisis UCMI Malaysia yang berjumlah 51 pasien. Hasil dari kegiatan ini menunjukkan peningkatan signifikan dalam pengetahuan peserta mengenai TB dari yang tidak paham sebesar 75% menjadi hanya 10% dan perilaku pencegahan setelah mengikuti program edukasi. Diharapkan bahwa dengan meningkatkan kesadaran masyarakat, kita dapat berkontribusi pada pengurangan angka kejadian TB dan mendukung upaya pemerintah dalam mencapai eliminasi TB di Malaysia.Abstract: The education program on the dangers of pulmonary tuberculosis (TB) to vulnerable communities in Malaysia aims to increase awareness and understanding of the disease and preventive measures. With the increasing number of TB cases in Malaysia, reaching 26,781 cases by 2022, including 2,623 deaths, this activity is crucial in TB elimination efforts. Through a participatory approach, education is conducted in the community with a focus on high-risk groups, such as individuals with underlying health conditions and those living in densely populated neighborhoods. The methods used included counseling, distribution of information materials, and the correct use of masks to ensure a good understanding of the symptoms, modes of transmission, and importance of mask use as well as adherence to treatment conducted at the UCMI Malaysia clinical service excellence center. Patients susceptible to infection by TB-causing bacteria, who are permanent patients with old age at the Center for the brilliance of hemodialysis clinic services UCMI Malaysia with a total of 51 patients. The results of this activity showed a significant improvement in the participants' knowledge of TB from 75% to only 10% of non-understanding and preventive behaviors after attending the education program. It is hoped that by increasing public awareness, we can contribute to the reduction of TB incidence and support the government's efforts in achieving TB elimination in Malaysia.