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All Journal Jurnal Labora Medika Medical Laboratory Analysis and Sciences Journal
Wildiani Wilson
Universitas Muhammadiyah Semarang
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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The activity of ethanol extract and aquadest of Jati Belanda (Guazuma ulmifolia Lamk) leaves against Candida albicans Imelda Putri Kusharyati; Muhammad Evy Prastiyanto; Wildiani Wilson; Nala Fidarotul Ulya
Medical Laboratory Analysis and Sciences Journal Vol 2 No 2 (2020): November 2020
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v2i2.57

Abstract

Fungi are the cause of infection, the development of fungal infections in Indonesia is influenced by environmental conditions so that microbes grow quickly and can increase significantly. Candida Albicans is a fungal disease that infects humans. As a country with a tropical climate, Indonesia has potential biodiversity as a medicine, one of which is Dutch teak (Guazuma ulmifolia Lamk) which has secondary metabolites that kill microbes that can be used as natural antifungals. The purpose of this study was to determine the antifungal activity of ethanol extract and distilled water of Dutch teak leaves on the growth of Candida Albicans. The method used is maceration with ethanol and distilled water solvents. The anti-fungal activity was evaluated by the good diffusion method with SDA media. The results of the antifungal activity of ethanol extract and distilled water of Dutch teak leaves against Candida Albicans were indicated by the formation of an inhibition zone around the wells on Candida Albicans ethanol extract concentrations of 25 mg / mL to 1000 mg / mL 8.6 mm - 15.6 mm, the distilled water extract has not formed a zone of inhibition at all concentrations. The conclusion is that the ethanol extract of Dutch teak leaves can inhibit the growth of Candida Albicans. while the distilled water extract was unable to inhibit the growth of Candida Albicans.
EFEKTIFITAS DAYA HAMBAT EKSTRAK ETANOL BAWANG DAUN (Aliium fistulosum. L) TERHADAP BAKTERI Salmonella typhi, Escherichia coli, DAN Staphylococcus aureus Akhmad Fauzan; Sri Sinto Dewi; Wildiani Wilson
Jurnal Labora Medika Vol 3, No 2 (2019): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.017 KB) | DOI: 10.26714/jlabmed.3.2.2019.54-57

Abstract

Bawang daun mengandung senyawa golongan flavonoid berupa kuersetin dan kaempferol. Senyawa yang terkandung pada bawang daun dapat menghambat pertumbuhan bakteri patogen salah satunya Staphylococcus aureus, Escherichia coli, dan Salmonella typhi. Tujuan penelitian ini untuk mengetahui daya hambat ekstrak etanol bawang daun terhadap pertumbuhan bakteri S. aureus, E. coli, dan S. typhi. Penelitian ini dilakukan secara eksperimen dengan menggunakan sampel bakteri S. aureus, E. coli dan S. typhi yang sudah disetarakan kekeruhannya dengan standar Mc. Farland 0,5. Pengujian daya hambat dengan menggunakan metode difusi sumuran pada media MHA dengan berat ekstrak etanol bawang daun 0,25 gram, 0,37 gram, 0,50 gram, 0,63 gram , dan 0,75 gram. Hasil zona hambat ekstrak etanol bawang daun pada berat 0,25 gram, 0,37 gram, 0,50 gram, 0,63 gram , dan 0,75 gram terhadap bakteri S. aureus  menunjukan hasil berturut-turut sebesar 0 mm, 15 mm, 15 mm, 16 mm, 18,3 mm. zona hambat terhadap bakteri bakteri E. coli pada berat yang sama berturut - turut sebesar 0 mm, 0 mm, 0 mm, 11 mm, dan 15,3 mm. Zona hambat terhadap bakteri  S. typhi pada berat yang sama berturut - turut sebesar. 13,3 mm, 19 mm, 19,6 mm, 22,3 mm, dan 25,6 mm.
Co-Authors Akhmad Fauzan Imelda Putri Kusharyati Nala Fidarotul Ulya Prastiyanto, Muhammad Evy Sri Sinto Dewi