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Potensi Isolat Bakteri Proteolitik dari Proses Pembuatan Pupuk Organik sebagai Starter Pengolahan Limbah Peternakan Nadhira Yahdiyani; Akhmad Hidayatulloh; Lilih Siti Nurhayati
Jurnal Teknologi Hasil Peternakan Vol 2, No 1 (2021): Maret
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.772 KB) | DOI: 10.24198/jthp.v2i1.31321

Abstract

Cattle farms produce waste in the form of feces and urine, the handling of this waste must be managed properly so that it can reduce the adverse effects. One way to use it is to process it into fertilizer. During the process of making microorganism fertilizer helps the process of degradation of complex compounds, one of which plays a role is proteo-lytic bacteria. The purpose of this study is to obtain proteo-lytic bacterial isolates that will be used as a starter in the manufacture of fertilizer. This research was conducted experimentally with research stages including isolation of proteolytic bacteria from the process of making fertilizer, measurement of proteolytic index and morphological cha-racterization of proteolytic bacteria microscopically and microscopically. From the results, 7 isolates of proteolytic bacteria with the largest proteolytic index were 1.51 of NS 3 isolates with the characteristics of small round colonies, flat edges, clear colors, flat surfaces and cell characteristics of bacilli and gram negative isolates.
Isolasi dan Seleksi Bakteri Kandidat Selulolitik dari Proses Pembuatan Pupuk Organik pada Pengolahan Limbah Peternakan Akhmad Hidayatulloh; Nadhira Yahdiyani; Lilih Siti Nurhayati
Jurnal Teknologi Hasil Peternakan Vol 3, No 2 (2022): September
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v3i2.41774

Abstract

Cattle farming is one of the livestock business commodities in Indonesia. In the production process, cattle farms produce waste in the form of feces and leftover feed which can be processed in such a way through a decomposition process into organic fertilizer. The rate of the decomposition process is influenced by the existing decomposer microbes. One of the microbes needed in the decomposition process is cellulolytic bacteria. The research was conducted to obtain potential cellulolytic bacterial isolates from the compost which can then be used as a starter in the decomposition process. This research was conducted experimentally with the stages of research including isolation of bacteria from decomposers, the isolates obtained were then reviewed on Nutrient Agar media to produce uniform colonies. Furthermore, screening was carried out to obtain cellulolytic bacteria using NA media added with 1% CMC (Carboxymethyl Cellulose). Based on the results of the study, obtained 7 bacterial isolates that have various cellulolytic indices. The best cellulolytic index was produced by isolates NC-12, NC-17, and NC-16.