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All Journal Pharmasipha Journal Syifa Sciences and Clinical Research (JSSCR)
Muhamad Handoyo Sahumena
Jurusan Farmasi, Fakultas Farmasi, Universitas Halu Oleo
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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STUDI POLA FRAGMENATASI JAMU TERKONFIRMASI PARASETAMOL MENGGUNAKAN LIQUID CHROMATOGRAPHY MASS SPECTROSCOPY (LCMS) Muhammad Taupik; Endah Djuwarno; Muhamad Handoyo Sahumena
Pharmasipha: Pharmaceutical Journal of Islamic Pharmacy Vol 4, No 2 (2020): September
Publisher : University Of Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.223 KB) | DOI: 10.21111/pharmasipha.v4i2.4558

Abstract

Salah satu bahan kimia obat yang sering ditambahkan pada jamu adalah parasetamol karena obat ini merupakan obat analgetik-antipiretik. Sampel terdiri atas 6 sampel jamu  dengan merek berbeda. Analisis kualitatif dengan metode KLT menggunakan fase gerak n-heksan:etil asetat (1:1) diperoleh tiga sampel yang positif mengandung parasetamol yakni sampel D, E, dan F. Ketiga sampel yang positif mengandung parasetamol dihitung kadarnya dengan metode LCMS. Analisis kuantitatif dengan LCMS menggunakan fase terbalik dengan fase gerak asetonitril:air (15%>:85%> v/v) pada laju alir 0.2 mL/menit dan volume injeksi 5 gL. Berdasarkan data kromatogram LCMS diperoleh waktu retensi (Rf) parasetamol 1,038 menit. Rata-rata waktu  retensi  sampel  jamu  mendekati   waktu  retensi parasetamol. Pendekatan selanjutnya berdasarkan pola fragmentasi dari Spektroskopi Massa. Berdasarkan data spektrum massa diperoleh fragmentasi dari parasetamol (152 m/z) membentuk  fragmen yakni p-aminofenol (109 m/z) dan asetalaldehid (44 m/z). Ion p-aminofenol,  menghasilkan subfragmen aldehyde (32 m/z) dan cyclopentadienylidene  (81 m/z). Berdasarkan hasil analisis menggunakan metode LCMS diperoleh empat sampel yang mengandung parasetamol, Sampel D. E dan F. Seharusnya ketiga sampel tersebut tidak diperbolehkan diperjualbelikan dan dikonsumsi karena  berdasarkan Peraturan  Menteri  Kesehatan  No. 006 Tahun 2012 pasal 33 dan pasal 37 tentang industri dan usaha obat tradisional bahwa obat tradisional dilarang mengandung bahan kimia hasil isolasi atau sintetik yang berkhasiat obat. Meski kandungan parasetamol dalam tergolong sedikit, tetapi menurut Permenkes RINo. 007 tahun 2012 bahwa bahan kimia obat mutlak tidak diperbolehkan terdapat dalam obat tradisional.
IDENTIFIKASI JAMU YANG BEREDAR DI KOTA KENDARI MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS Muhamad Handoyo Sahumena; Ruslin Ruslin; Asriyanti Asriyanti; Endah Nurrohwinta Djuwarno
Journal Syifa Sciences and Clinical Research Vol 2, No 2 (2020): Volume 2 Edisi 2 2020
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v2i2.6977

Abstract

Jamu is a traditional medicine that contains ingredients or ingredients derived from plants, animals, minerals, or mixtures of these ingredients that have been hereditary for medicinal use. However, some industry players add Medicinal Chemicals (BKO) such as mefenamic acid into herbal medicine. This study aims to determine the validity of the method in the analysis of mefenamic acid by UV-Vis spectrophotometry on herbs circulating in several markets in Kendari City. The sampling technique used in this study is purposive sampling method so that it gets 5 herbal samples. The study began with method validation to ensure the accuracy of the method in determining the level of mefenamic acid in the sample. The results of the method validation show that this method is good for detecting the presence of mefenamic acid BKO in herbal medicine with a validation parameter value that is the correlation value (r) of 0.998; detection limit (LOD) 0.48 µg / mL; limit of quantification (LOQ) 1.63 µg / mL; intraday and interday precision expressed with the value of relative standard deviation% respectively 0.014% and 0.013%; and the accuracy stated in% recovery is 95.41% (80%), 99.04% (100%), and 102.5% (120%). The results of the analysis of the sample using a validated method showed that there were herbs with mefenamic acid BKO content of 0.8%.
Formulasi Self-Nanoemulsifiying Drug Delivery System (SNEDDS) Asam Mefenamat menggunakan VCO dengan Kombinasi Surfaktan Tween dan Span Muhamad Handoyo Sahumena; Suryani Suryani; Neni Rahmadani
Journal Syifa Sciences and Clinical Research Vol 1, No 2 (2019): Volume 1 Edisi 2 2019
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.66 KB) | DOI: 10.37311/jsscr.v1i2.2660

Abstract

Mefenamic acid is a non-steroidal anti-inflammatory drug (NSAID) which has analgesic, anti-inflammatory and antipyretic effects. Mefenamic acid works by inhibiting prostaglandin synthesis as an inflammatory mediator. Mefenamic acid has low drug solubility and a long process of dissolution in the body which greatly affects the speed of absorption and bioavailability of the drug. In this study, mefenamic acid nanoemulsion formulation was carried out through a Self-Nanoemulsifying Drug Delivery System (SNEDDS) delivery system. SNEDDS is a drug delivery method through isotropic oil extraction, surfactants, cosurfactans and drug that form oil in water (m/a) emulsions which when in contact with the water phase in the digestive tract wiil from a nanoemulsion that occurs spontaneously so that the drug dissolves with a particle size small so as to increase the effective surface area for absorption. The purpose of the study was to determine the ratio of surfactant and cosurfactant composition to the optimum formula of SNEDDS of mefenamic acid with VCO as an oil phase. The SNEDDS formula was obtained by mixing the surfactants tween 80 and span 80, cosurfactant PEG 400 and VCO as the oil phase using the characterization of determining the optimum formula, namely emulsion formation, transmittance and emulsification time. The composition of the optimum formula of SNEDDS of mefenamic acid is 1 mL VCO; 1 mL PEG 400; 6 mL tween 80; 1 mL span 80. Optimum formula showed clear emulsion results, with transmittance values of 89,04% and emulsification time under 1 minute. In this study produced the optimum formula SNEDDS the met the criteria based on droplet size parameters of 153,5 nm, potential zeta value of 8,2 mV and showed good stability.
IDENTIFIKASI SENYAWA FLAVANOID DAUN SEMBUNG (Blumea balsamifera L.) Ahmad Ruhardi; Muhamad Handoyo Sahumena
Journal Syifa Sciences and Clinical Research Vol 3, No 1 (2021): Volume 3 Edisi 1 2021
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v3i1.9925

Abstract

The purpose of this study was to determine the levels of flavonoids contained in the sembung leaf methanol extract. To identify the flavonoid content, Spectrophotometry UV-Vis method. Further, the analysis of methanol extract flavonoid in sembung leaves was carried out at a wavelength of 382 nm with successive absorbance values of 0.094; 0.090; 0.084. The total content of flavonoids in the sample was calculated by calibrating the absorbance value of the example with a standard linear equation of quercetin, y = 0.060 x -0.016 with a correlation coefficient (R2) = 0.997, and; the average total flavonoid content in the methanol extract of the leaves was 0.175%.
Studi Pola Fragmentasi Jamu Terkonfirmasi Parasetamol Menggunakan Liquid Chromatography Mass Spectroscopy (LCMS) Muhammad Taupik; Endah Djuwarno; Muhamad Handoyo Sahumena
Pharmasipha : Pharmaceutical Journal of Islamic Pharmacy Vol 4, No 2 (2020): September
Publisher : University Of Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/pharmasipha.v4i2.4558

Abstract

Salah satu bahan kimia obat yang sering ditambahkan pada jamu adalah parasetamol karena obat ini merupakan obat analgetik-antipiretik. Sampel terdiri atas 6 sampel jamu  dengan merek berbeda. Analisis kualitatif dengan metode KLT menggunakan fase gerak n-heksan:etil asetat (1:1) diperoleh tiga sampel yang positif mengandung parasetamol yakni sampel D, E, dan F. Ketiga sampel yang positif mengandung parasetamol dihitung kadarnya dengan metode LCMS. Analisis kuantitatif dengan LCMS menggunakan fase terbalik dengan fase gerak asetonitril:air (15%>:85%> v/v) pada laju alir 0.2 mL/menit dan volume injeksi 5 gL. Berdasarkan data kromatogram LCMS diperoleh waktu retensi (Rf) parasetamol 1,038 menit. Rata-rata waktu  retensi  sampel  jamu  mendekati   waktu  retensi parasetamol. Pendekatan selanjutnya berdasarkan pola fragmentasi dari Spektroskopi Massa. Berdasarkan data spektrum massa diperoleh fragmentasi dari parasetamol (152 m/z) membentuk  fragmen yakni p-aminofenol (109 m/z) dan asetalaldehid (44 m/z). Ion p-aminofenol,  menghasilkan subfragmen aldehyde (32 m/z) dan cyclopentadienylidene  (81 m/z). Berdasarkan hasil analisis menggunakan metode LCMS diperoleh empat sampel yang mengandung parasetamol, Sampel D. E dan F. Seharusnya ketiga sampel tersebut tidak diperbolehkan diperjualbelikan dan dikonsumsi karena  berdasarkan Peraturan  Menteri  Kesehatan  No. 006 Tahun 2012 pasal 33 dan pasal 37 tentang industri dan usaha obat tradisional bahwa obat tradisional dilarang mengandung bahan kimia hasil isolasi atau sintetik yang berkhasiat obat. Meski kandungan parasetamol dalam tergolong sedikit, tetapi menurut Permenkes RINo. 007 tahun 2012 bahwa bahan kimia obat mutlak tidak diperbolehkan terdapat dalam obat tradisional.
Co-Authors ,, Suryani Ahmad Ruhardi Asriyanti Asriyanti Endah Nurrohwinta Djuwarno Muhammad Taupik Neni Rahmadani Ruslin Ruslin