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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan
Subaryono Subaryono
Balai Besar Penelitian dan Pengembangan Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Isolasi dan Identifikasi Bakteri Penghasil Alginat Lyase dari Rumput Laut Sargassum crassifolium Subaryono Subaryono; Rosmawaty Peranginangin; Maggy Thenawidjaja Suhartono; Fransiska Rungkat Zakaria
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): Juni 2015
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v10i1.239

Abstract

Penelitian tentang isolasi dan identifikasi bakteri penghasil alginat lyase dari rumput laut coklat Sargassum crassifolium sudah dilakukan. Rumput laut didekomposisi selama 5 hari, dan bakteri dikultur dalam media luria bertani agar yang mengandung alginat 1 g/100 ml. Setelah diinkubasi selama 3 hari, bakteri yang tumbuh diisolasi dan diseleksi untuk mendapatkan isolat yang positif menghasilkan alginat lyase. Aktivitas alginat lyase ditandai dengan adanya zona bening di sekitar koloni setelah ditetesi dengan larutan 10% setil piridinium klorida. Hasil penelitian menghasilkan 4 isolat bakteri dengan indeks alginolitik tertinggi yaitu S245, S235, S155, dan S215. Identifikasi ke-empat isolat dilakukan secara morfologi, biokimia, dan genetik. Pelitian menunjukkan bahwa isolat S245 memiliki kemiripan dengan Bacillus megaterium, S235 memiliki kemiripan dengan Bacillus thuringiensis, S155 memiliki kemiripan dengan Bacillus cereus, dan S215 memiliki kemiripan dengan Bacillus pseudomycoides.
Distribution of brown seaweed producing alginate in Indonesia and the potential utilization Subaryono Subaryono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 2 (2011): August 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v6i2.61

Abstract

Brown seaweed producing alginate frequently obtained in Indonesian waters , especially fromgenus of Sargassum and Turbinaria. These seaweeds are wide distributed from Aceh to Papuawaters. There are 15 species of Sargassum and some species of Turbinaria in Indonesia. Thisseaweed were very potential used as a row material of alginate industry, with main market Chinaand Malaysia. Quality requirement for seaweed as row material for alginate industry in China werealginate content not less than 17%, viscosity 47 cP, and gel strength 139 g/cm2. Expansion ofalginate industry in Indonesia was limited by low continuity of row material production. Prospectiveutilization of this brown seaweed as a row material of drug/traditional herbal medicine were sohigh because its ability to reducing high blood glucose in diabetes patient and decelerate thegrowth of cancer cell. Natural seaweed collection were done at peak season production on dryseason, and help out to increase the welfare of seaweed collection farmer.
Alginates modification and the prospective uses of their products. Subaryono Subaryono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 1 (2010): May 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v5i1.40

Abstract

Alginate is a natural hydrocolloid that is used in food and non food industries. The weakness ofnative alginate cause the limited uses of this material in industry. Some weaknesses of nativealginate had been successfully overcome either by the modification of alginate structure orinteraction of alginate with another substances. The low solubility of alginate and its low stabilityagainst acid had been successfully resolved with esterification of alginate backbone with propyleneglycol, producing propylene glycol alginate (PGA). The high syneresis of alginate gel had beensuccessfully overcome with partial hydrolysis, producing short chain alginate, introduce shortchain polyguluronate and interact alginate with other substances such as locust bean gum (LBG).Alginate hydrophilic properties could be changed into amphiphilic by long alkyl chain substitutiontowards part of the polysaccharides group. The ability of alginate to promote the growth of probioticbacteria could be done by enzymatic hydrolysis of alginate using alginate lyase producingalginate oligosaccharides (AOS). The modification of alginate had opened the big opportunity forthe uses of alginate and its derivatives, both in food and non food sectors.
Co-Authors Fransiska Rungkat Zakaria Maggy Thenawidjaja Suhartono Rosmawaty Peranginangin