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All Journal FITOFARMAKA: Jurnal Ilmiah Farmasi EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Sutanto Sutanto
Program Studi Kimia FMIPA Universitas Pakuan
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Mathematics Medicine & Pharmacology

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PENGARUH BERBAGAI METODE EKSTRAKSI PADA PENENTUAN KADAR FLAVONOID EKSTRAK ETANOL DAUN ILER (Plectranthus scutellarioides) Novi Fajar Utami; Sutanto Sutanto; Sely Meidi Nurdayanty; Usep Suhendar
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 10, No 1 (2020): FITOFARMAKA | Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.186 KB) | DOI: 10.33751/jf.v10i1.2069

Abstract

Tanaman iler merupakan tanaman yang berfungsi sebagai tanaman obat tradisional. Senyawa flavonoid pada tanaman iler memiliki aktivitas sebagai antioksidan. Kadar flavonoid pada ekstrak salah satunya dipengaruhi oleh metode ekstraksi yang digunakan. Penelitian ini bertujuan untuk menentukan metode ekstraksi yang terbaik untuk menghasilkan kadar flavonoid tertinggi dalam ekstrak etanol 70% daun iler. Metode ekstraksi yang digunakan meliputi Maserasi, Refluks, Microwave Assisted Extraction (MAE), dan Ultrasound-Assisted Extraction (UAE). Penetapan kadar flavonoid ekstrak etanol 70% daun iler dilakukan secara spektrofotometri UV-Vis menggunakan pereaksi AlCl3. Hasil penelitian menunjukkan bahwa metode ekstraksi yang terbaik untuk menghasilkan kadar flavonoid tertinggi dalam ekstrak etanol 70% daun iler yaitu metode Microwave Assisted Extraction. Kadar flavonoid dengan metode maserasi, refluks, Microwave Assisted Extraction dan Ultrasound Assisted Extraction berturut-turut sebesar 0,41%, 0,45%, 0,75%, dan 0,62%.
VALIDASI METODE UJI KADMIUM DALAM AIR SUMUR SECARA SPEKTROFOTOMETRI SERAPAN ATOM Ade Heri; Sutanto Sutanto
EKOLOGIA Vol 16, No 1 (2016): JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.054 KB) | DOI: 10.33751/ekol.v16i1.60

Abstract

This validation is refer to SNI 17025:2008 which detail linearity, precision, and accuracy that conducted 7 times. The detection limit is taken from making standard by using AAS graphic from PG type 990 and then the result used for analyzing of cadmium in well water. 2 liter of the sample (that take from one well in designated area) is aspired into AAS with 228,8 nm wave length. According to the result of this method, got the linearity with coefficient regretion value 0,9997, detection limit 0,0028 mg/L, precision of unspike sample with RSD value RSD 10,77 % and precision of spike sample with RSD 0,16 %, and recovery for accuracy value 86,44 % - 90,30 %. This method give the result the meet the requirement for validation applied, so that the method can be used for routine of cadmium content in well water.Average of cadmium content in well water is 0,0247 mg/L.Keyword : Validation methode, Cadmium, Well water, Atomic Absorption Spectrophotometer
VALIDASI METODE UJI KADMIUM DALAM AIR SUMUR SECARA SPEKTROFOTOMETRI SERAPAN ATOM Ade Heri; Sutanto Sutanto
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 16, No 1 (2016): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v16i1.60

Abstract

This validation is refer to SNI 17025:2008 which detail linearity, precision, and accuracy that conducted 7 times.  The detection limit is taken from making standard by using AAS graphic from PG type 990 and then the result used for analyzing of cadmium in well water.  2 liter of the sample (that take from one well in designated area) is aspired into AAS with 228,8 nm wave length.    According to the result of this method, got the linearity with coefficient regretion value 0,9997, detection limit 0,0028 mg/L, precision of unspike sample with RSD value RSD 10,77 % and precision of spike sample with RSD 0,16 %, and recovery for accuracy value 86,44 % - 90,30 %.  This method give the result the meet the requirement for validation applied, so that the method can be used for routine of cadmium content in well water.Average of cadmium content in well water is 0,0247 mg/L. Keyword : Validation methode, Cadmium, Well water, Atomic Absorption Spectrophotometer
Co-Authors Ade Heri Novi Fajar Utami Sely Meidi Nurdayanty Usep Suhendar