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All Journal Jurnal Pengolahan Pangan BIOEDUPAT: Pattimura Journal of Biology and Learning Pakem : Jurnal Pengabdian Kepada Masyarakat German für Gesellschaft (J-Gefüge)
Ferymon Mahulette
Universitas Pattimura
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Public Health Social Sciences Other

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Decreasing SGPT level and macrophage activity through CD68 expression in the Balb/c mice (Mus musculus) liver infected with Salmonella typhi after treating with atung seeds (Parinarium glaberimmun Hassk) Eifan Boyke Pattiasina; Pieter Kakisina; Ferymon Mahulette
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 1 No 1 (2021): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.827 KB) | DOI: 10.30598/bioedupat.v1.i1.pp17-23

Abstract

Normally macrophages are always in the body and spread in various body tissues such as liver tissue (Kupffer cells). Macrophages in tissue can be identified by the expression of several markers, in humans the marker is CD68. The increase and decrease in macrophage activity in the liver can also be indicated by an increase in SGPT levels so that atung seeds have the ability to inhibit the growth of S. tyhpi bacteria which contain tannin compounds which can damage microbial cell walls and form bonds with microbial functional proteins so that bacterial growth is inhibited. The purpose of this study was to determine the SGPT levels and macrophage activity. The method used is laboratory experiment. The results showed an increase in SGPT levels in the positive control (87.00 ± 2,915) and a concentration of 25% (84.20 ± 3,962) and a decrease in SGPT levels in the negative control (50.80 ± 2.168 *), 50% concentration (78.20 ± 3.114 *) and concentrations. 75% (58.20 ± 3,834), decreased macrophage activity in the liver also occurred at a concentration of 50% and at a concentration of 75%, the liver was normal, which was indicated by the resulting brown expression
KARAKTERISTIK MIKROBIOLOGI DAN KIMIA INASUA IKAN BABI (RUVETTUS TYDEMANI WEBER) DENGAN DAN TANPA NIRA KELAPA Ferymon Mahulette; Tri Santi Kurnia
Jurnal Pengolahan Pangan Vol 6 No 1 (2021)
Publisher : Fakultas Pertanian Universitas Alkhairaat Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/pangan.v6i1.41

Abstract

Inasua ikan babi (Ruvettus tydemani) adalah produk fermentasi ikan yang sangat digemari masyarakat Teon, Nila, dan Serua (TNS) di Maluku Tengah. Produk fermentasi ini diolah dengan menambahkan garam saja (inasua tanpa nira) atau garam dan nira kelapa (inasua nira). Inasua ikan babi sejak lama dikonsumsi masyarakat TNS tetapi karakteristik mikrobiologi dan kimia produk fermentasi ini belum pernah diteliti. Penelitian ini bertujuan untuk mendeskripsikan karakteristik mikrobiologi dan kimia inasua ikan babi untuk mengetahui kelayakan produk ini sebelum dikonsumsi. Analisis Mikrobiologi menggunakan metode hitungan cawan pada media Nutrient agar (NA), Mannitol Salt Agar (MSA), de Man, Rogosa and Sharpe Agar (MRSA) dan Eosin Methylene Blue Agar (EMBA) sedangkan analisis kimia meliputi uji kadar histamin, Total Volatile Base-Nitrogen (TVB-N), Trimethylamine-Nitrogen (TMA-N), asam lemak bebas dan mineral. Sebanyak 7 isolat ditemukan pada inasua tanpa nira, sedangkan inasua nira hanya ditemukan 6 isolat yang didominasi oleh Staphylococcus dan Bacillus. Kadar TVB-N dan TMA-N inasua tanpa nira sedikit lebih tinggi dibanding inasua nira, sebaliknya kadar histamin dan asam lemak bebas inasua nira lebih tinggi dibandingkan inasua tanpa nira. Secara umum inasua nira memiliki jumlah bakteri patogen lebih sedikit dibandingkan inasua nira, sebaliknya karakteristik kimia inasua tanpa nira lebih aman untuk dikonsumsi dibandingkan inasua nira.
SAINS ITU MENYENANGKAN: ASISTENSI MENGAJAR IPA PADA SMP DI KECAMATAN LEIHITU Ferymon Mahulette; Esmi Hajan; Irwina Irwina
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 3 No 1 (2023): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.3.1.1-6

Abstract

Science learning is still difficult to understand for Junior High School students in Leihitu District, Ambon Island. The main cause are the teacher's lack of creativity to designing concrete science lessons and incomplete laboratory facilities. This problem was slightly resolved with practical-based learning in the Lecturer Teaching Assistance of Pattimura University in 2022. The activity was carried out 4 times in class IX students at SMPN 103 Central Maluku and SMP Muhammadyah Mamala on December 13-17 2022. The topic being taught was electricity static and dynamic in life, magnetism and its uses, biotechnology and land and the sustainability of life. The mean scores of the two schools' student satisfaction levels for this activity were 3,401 and 3,408, respectively, in the strongly agree category. The continuation of Lecturer Teaching Assistance needs to be carried out because it is very beneficial to improve the quality of science learning in schools
Potential of vegetable waste as alternative production bioelectricity Yulviany Latuihamallo; Ferymon Mahulette; Theopilus Wilhelmus Watuguly
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 3 No 1 (2023): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v3.i1.pp90-94

Abstract

Energy use is closely related to world economic growth and urbanization. Therefore, research was carried out by utilizing organic vegetable waste as a source of renewable energy. This study aims to determine variations in incubation time of vegetable waste that affect electricity production and to determine differences in bio-electricity production based on variations in incubation time of vegetable waste. The results of bioelectricity measurements in the Microbial Fuel Cell reactor, the maximum production of bioelectricity is found at the incubation period of 7 days, namely 9.48 mA at the 0th minute (initial), the maximum electric voltage is 288 mV at the 60th minute, the maximum power is 2205.50 mW in the 30th minute, and the maximum power density is 152.10 mW/m2 in the 30th minute.
Analysis of coliform bacteria contamination in drinking water refill in Ambon City Monica Nikijuluw; Mery Pattipeilohy; Ferymon Mahulette
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 3 No 2 (2023): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v3.i2.pp125-128

Abstract

In recent years, refillable drinking water businesses have grown rapidly in several cities in Indonesia, including Ambon City. On the one hand, Refillable Drinking Water Depots (DAMIU) support efforts to realize a healthy society by expanding the reach of clean water consumption, On the other hand, DAMIU tend to be problematic when faced with business interests. Feces contain coliform bacteria which is an indicator of water contamination. This study aims to determine the presence of coliform bacteria in Ambon City. The samples used in the study came from three sub-districts of refill drinking water depot sources, namely the Teluk Dalam sub-district, Nusaniwe sub-district, and Baguala sub-district. Microbiological testing of refill drinking water using MPN (most probable number) method. The MPN test results showed that three refill drinking water samples were not contaminated with coliform bacteria because there was no turbidity or gas formation in the Durham tube for gas-forming bacteria. This indicates that the three refill drinking water samples tested microbiologically have good quality and are safe for consumption, because in 100 ml of water no coliform bacteria were found in accordance with Permenkes No. 492/Menkes/Per/IX/2010 regulations on the requirements and supervision of water quality, which states that the microbiological requirements for drinking water are MPN Coliform /100 ml sample = 0. There are no coliform bacteria in the refillable drinking water depot in Ambon City so the water is suitable for consumption
PELATIHAN PEMBUATAN MEDIA PETUMBUHAN MIKROBA UNTUK MENINGKATKAN WORK SKILL GURU, RESEARCH SKILL SISWA JURUSAN IPA Mery Pattipeilohy; Ferymon Mahulette; Alamanda Pelamonia
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2024): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.4.1.127-133

Abstract

Permasalahan yang sering dijumpai di sekolah-sekolah terkait pelaksanaan praktikum adalah minimnya peralatan karena kurangnya sarana dan prasana penunjang kegiatan praktikum, kurangnya kreativitas dalam menyusun topik praktikum dan minimnya pengatahuan guru dalam memanfaatkan bahan-bahan alami yang dapat dijadikan sebagai bahan alternatif dalam pelaksanaan proses praktikum. Permasalahan inilah yang dialami oleh guru biologi pada SMA Negeri 1 Seram Bagian Barat, dimana khususnya untuk konsep mikrobiologi tidak di lakukan praktikum, dikarenakan dalam pelaksanaannya membutuhkan alat dan bahan yang banyak juga proses kerja yang harus steril. Tetapi karena alat dan bahan yang tidak lengkap dan bahkan tidak tersedia karena bahan untuk pertumbuhan mikroba cukup mahal dan butuh waktu yang lama dalam proses inkubasi serta pengamatan mikroba (bakteri dan kapang) yakni memerlukan waktu 2-3 hari. Hal ini yang menyebabkan guru mengalami kesulitan untuk melakukan praktikum terkait konsep mikrobiologi sehingga prakitkum belum pernah dilakukan. Oleh karena itu, tujuan dari pelaksanaan pengabdian ini adalah untuk meningkatkan work skill guru dan research skill siswa pada sekolah tersebut melalui pendampingan dan pelatihan menggunakan draft penuntun praktikum. Berdasarkan hasil yang diperoleh diketahui bahwa kemampuan kerja dan keterampilan guru dan juga kemampuan siswa semakin meningkat.
Edukasi EDUKASI VIDEO PRODUK PANGAN KACANG GUDE BAGI GURU KABUPATEN BURU SELATAN Alwi Smith; Kristin Sangur; Ferymon Mahulette; Ritha Lusian Karuwal
German für Gesellschaft (J-Gefüge) Vol 2 No 1 (2023): German für Gesellschaft (J-Gefüge)
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jgefuege.2.1.51-60

Abstract

Currently public education can be done through the use of video. This is done so that activities run smoothly and save time, as well as benefit people who are not familiar with the potential of pigeon pea. Pigeon pea as a functional plant for the Kisar people can be processed into functional food products such as sweet soy sauce, jam, sweetened condensed milk, pigeon pea extract, gude crackers, and cajanus bar. For this reason, this video is very important to do to explain the tools and materials that need to be prepared and how to make these food products to Science/Biology and Craft teachers. This educational activity method is carried out through preparation and implementation activities for one month. The results obtained are that science/biology and craft teachers can get to know pigeon pea, understand tools and materials and how to make functional food products. Recommendations for this educational activity can be implemented in the curriculum for crafts at the junior and senior high school levels
Co-Authors Alamanda Pelamonia Alwi Smith Eifan Boyke Pattiasina Esmi Hajan Irwina Irwina Kristin Sangur Mery Pattipeilohy Monica Nikijuluw Pieter Kakisina Ritha Lusian Karuwal Theopilus Wilhelmus Watuguly Tri Santi Kurnia Yulviany Latuihamallo