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All Journal Indonesian Journal of Global Health research Journal of Nursing and Health (JNH) Akper Yakpermas Banyumas BIOEDUPAT: Pattimura Journal of Biology and Learning Jurnal Media Analis Kesehatan
Didik Wahyudi
Sekolah Tinggi Ilmu Kesehatan Nasional
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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Bacterial contamination test on plates, spoons, and glass at a food stall (angkringan) Surakarta City, Central Java Dara Akwila; Aji Tri Aminougroho; Didik Wahyudi
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 2 No 1 (2022): Bioedupat: Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v2.i1.pp19-22

Abstract

Cutlery is an important component that can affect the health of the digestive tract. Plates, glasses and spoons are eating utensils that are often used by the community. In the city of Surakarta, Central Java, there are many roadside food stalls that are in great demand by the community, namely Angkringan. The purpose of this study was to determine bacterial contamination of cutlery (pring, glass and spoon) at a food stall (Angkringan) in Surakarta City, Central Java. This research is a descriptive observational study, bacteriological examination of cutlery begins with swab sampling, bacterial examination using the Total Plate Number method, and confirmed by Gram staining. The results of the examination of the number of bacterial colonies were compared with the standard reference of Permenkes No. 1096/MENKES/PER/VI/2011, which is 0 colonies/cm2. The results showed the average bacterial contamination in angkringan food stalls in Surakarta City, as follows: plate 10230 colonies/cm2, glass 9560 colonies/cm2 and spoon 3960 colonies/cm2 . All cutlery that was inspected did not meet the standards of Permenkes No. 1096/MENKES/PER/VI/2011.
Detection of Gene Alg8 and Alg44 in Clinical Isolates Pseudomonas Aeruginosa using Plymerase Chain Reaction Method Didik Wahyudi; Wimpy Wimpy; Dewi Saroh
Indonesian Journal of Global Health Research Vol 4 No 4 (2022): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.419 KB) | DOI: 10.37287/ijghr.v4i4.1292

Abstract

The alg8 and alg44 genes are one of the genes that control alginate production in Pseudomonas aeruginosa bacteria, these genes are one of the main virulence factors causing chronic infections in the human body. Pseudomonas aeruginosa is a bacterium that causes infections in several cases in various parts of the body. The purpose of this study was to detect the presence of alg8 and alg44 genes in several isolates of Pseudomonas aeruginosa from several clinical samples (urine, sputum, and pus) using the Polymerase Chains Reaction method. The study was initiated by characterizing and purification of 6 isolates of Pseudomonas aeruginosa from urine, sputum, and pus samples (2 isolates each), identification of isolates was carried out by biochemical tests. Bacterial DNA isolation was carried out using the DNeasy Blood and Tissue Kit, the results of the isolation were tested by electrophoresis. Six samples of Pseudomonas aeruginosa DNA were tested for the presence of alg8 and alg44 genes by PCR method. The primary design was carried out using the website https://www.ncbi.nlm.nih.gov. The alg8 gene as a whole consists of 1214 nitrogenous bases, the primer used produces an amplicon of 882 bp (72.6%), alg44 gene consists of 818bp, the primer used amplifies 316 bp (36%). alg8 and alg44 genes were found in all isolates of Pseudomonas aeruginosa.
Detection of Gene Alg8 and Alg44 in Clinical Isolates Pseudomonas Aeruginosa using Plymerase Chain Reaction Method Didik Wahyudi; Wimpy Wimpy; Dewi Saroh
Indonesian Journal of Global Health Research Vol 4 No 4 (2022): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.419 KB) | DOI: 10.37287/ijghr.v4i4.1292

Abstract

The alg8 and alg44 genes are one of the genes that control alginate production in Pseudomonas aeruginosa bacteria, these genes are one of the main virulence factors causing chronic infections in the human body. Pseudomonas aeruginosa is a bacterium that causes infections in several cases in various parts of the body. The purpose of this study was to detect the presence of alg8 and alg44 genes in several isolates of Pseudomonas aeruginosa from several clinical samples (urine, sputum, and pus) using the Polymerase Chains Reaction method. The study was initiated by characterizing and purification of 6 isolates of Pseudomonas aeruginosa from urine, sputum, and pus samples (2 isolates each), identification of isolates was carried out by biochemical tests. Bacterial DNA isolation was carried out using the DNeasy Blood and Tissue Kit, the results of the isolation were tested by electrophoresis. Six samples of Pseudomonas aeruginosa DNA were tested for the presence of alg8 and alg44 genes by PCR method. The primary design was carried out using the website https://www.ncbi.nlm.nih.gov. The alg8 gene as a whole consists of 1214 nitrogenous bases, the primer used produces an amplicon of 882 bp (72.6%), alg44 gene consists of 818bp, the primer used amplifies 316 bp (36%). alg8 and alg44 genes were found in all isolates of Pseudomonas aeruginosa.
Sputum Sample Quality and Delay of TCM Examination and Microscopic Results from Tuberculosis Suspect Patient Yusianti Silviani; Ardy Prian Nirwana; Didik Wahyudi
Jurnal Media Analis Kesehatan Vol 14 No 2 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v14i2.319

Abstract

Tuberculosis (TB) is a disease with a mortality rate equivalent to 11 deaths/hour caused by Mycobacterium tuberculosis. Technological developments have been able to detect TB with the GeneXpert® MTB/RIF TCM (Rapid Molecular Test) examination. The aim of this study was to determine the effect of sputum sample quality and examination delays on TCM and microscopic results in patients suspected of having tuberculosis. The research design used in this study used experimental analytics with a cross-sectional approach. The sample in this study was 40 samples taken using quota sampling. Samples were examined using Microscopic and TCM methods. In the microscopic method, 3 treatment delays are carried out, namely immediately, 5 hours and 24 hours. The results showed that all samples experienced a decrease in the number of BTA + but a decrease in grade did not occur in all samples. The decrease in consistency began to appear at a delay of 5 hours. A decrease in BTA microscopic grading often occurs when the delay is from 5 hours to 24 hours. From the results of the Friedman test, a value of α= 0.000 (< 0.05) was obtained so that it can be concluded that there is no difference in the results of microscopic examination with TCM, but there is an influence of sputum quality on BTA results (+) and there is an influence of delay time on microscopic examination of BTA.
HUBUNGAN POLIMORFISME GEN MTHFR C677T DENGAN KADAR TRIGLISERIDA DARAH PADA PASIEN JANTUNG KORONER Didik Wahyudi
Journal of Nursing and Health Vol. 9 No. 4 (2024): Journal of Nursing and Health
Publisher : Yakpermas Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52488/jnh.v9i4.405

Abstract

Co-Authors Aji Tri Aminougroho Ardy Prian Nirwana Dara Akwila Dewi Saroh Wimpy, W Yusianti Silviani