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Journal : METANA

OPTIMASI PRODUKSI PROTEIN SEL TUNGGAL DARI BAGASE TERHIDROLISA DENGAN FERMENTASI OLEH SACCAROMYCES CEREVICEAE Isti Pudjihastuti; Margaretha T S; Wahyuningsih Wahyuningsih; Edy Supriyo
METANA Vol 8, No 02 (2012): Desember 2012
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.631 KB) | DOI: 10.14710/metana.v8i02.6810

Abstract

The Came pulp (bagase) were main contained cellulose, through process delignification and hidrolized can be used to growting sacaromyces cereviceae yeast as fermented media, so can produced biomass microbial as known as single cell protein (SCP).Design experiment was random block with treatment nutrient added as long as fermented process. The measured parameter were protein contained in microbial biomass with Kehjdahl method. Optimum result were formed to 8 days fermentation and nutrient added (NH4)2SO4:1 gr,MgSO47H2O : 0,5 gr, molase (tetes) 20% : 2 ml to media 50 gr. Key word: bagase, Saccaromyces Cereviceae,SCP
Perancangan Pengering Berinsulasi Dengan Aplikasi Pengeringan Ikan Tengiri Wahyuningsih Wahyuningsih; Isti Pudjihastuti
METANA Vol 12, No 2 (2016): Desember 2016
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1166.899 KB) | DOI: 10.14710/metana.v12i2.9750

Abstract

Prinsip Dasar Rancang Bangun Alat Pengering, peubah-peubah yang harus diperhatikan antara lain Sifat – sifat bahan yang akan dikeringkan,karakteristik pengeringan dari bahan, aliran panas, kualitas produk, fasilitas sekeliling,kapasitas alat,Dari faktor – faktor tersebut diharapkan untuk mendapatkan alat pengering dengan kinerja yang berdayaguna dan berhasil guna. Tujuan dari penelitian ini adalah merancang dan membangun alat pengering ikan berinsulasi untuk memperbaiki proses pengeringan ikan tradisional,meningkatkan nilai gizi, hygiene dan keamanan pangan dan mengaplikasikan alat pengering pada pengeringan ikan teri (Stolephorus heterolobua), serta mengetahui kwantitas asam lemak omega-3. Alat  pengering hasil rancang bangun, ukuran 1 m x 1,2m x1,75 m, ukuran kolektor 1,2 m x 0,6 m, dapat dimanfaatkan untuk mengeringkan ikan teri (Stolephorus heterolobua) secara higienis dan mempertahankan kualitas produk Lama pengeringan  72 jam memberikan hasil ikan kering yang memenuhi standar kadar air , untuk pemanas surya kadar air 2,45 %, untuk pemanas buatan kadar air 2,65% Produk yang disukai adalah pengeringan dengan kadar garam 6% Pada produk yang dapat diterima memberikan nilai EPA 0,5972 gr/100 gr,  dan DHA 0,4032 gr/100 gr, nilai TBA  1,21 mgr/kg, TVB : 7,04mgr    N/100gr,TMA : 5,11 % mgr N. Design of Insulated Dryers Application for Tengiri Fish Dryer  Basic Principles of Design Tools dryer, variables that must be considered among other properties - properties of the material to be dried, the characteristic drying of the material, heat flow, quality of products, the facilities around, the capacity of the tool, From factors - these factors are expected to get drier the performance of a very powerful and effective. The aim of this study was to design and build insulated fish dryers to improve the traditional fish drying process, enhance nutritional value, food safety and hygiene and to apply drier at drying anchovy (Stolephorus heterolobua), as well as to know the quantity of omega-3 fatty acids. Drier the result of design, size 1m x 1.2m x1,75 m, collector size of 1.2 mx 0.6 m, can be used for drying fish (Stolephorus heterolobua) hygienically and maintain the quality of products Lama drying 72 hours results dried fish that meets dtandar water content, for solar heating water content of 2.45%, for artificial heating water content of 2.65% preferred product is dried with saline 6% On acceptable products value EPA 0.5972 gr / 100 g and 0.4032 g DHA / 100 g, the value of TBA 1.21 mgr / kg, TVB: 7,04mgr N / 100gr, TMA: 5.11% N mgr 
ASIDOLISIS ENZIMATIK MINYAK IKAN TUNA( THUNNUS THYNNUS) MENJADI PRODUK ASAM LEMAK KAYA OMEGA-3 DENGAN PEMANFAATAN LIPASE GETAH PEPAYA (carica papaya latex) Wahyuningsih, Wahyuningsih; Pudjihastuti, Isti; Kusumayanti, Heny
METANA Vol 8, No 01 (2012): Juli 2012
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.392 KB) | DOI: 10.14710/metana.v8i01.4909

Abstract

Incorporation of omega-3 polyunsaturated acids (n-3 PUFA) into Tuna (Thunnus thynnus) fish oil was investigated by using acidolysis enzimatis Process. The product of this modification is expected to be used as an ingredient nutrifition food products, especially in milk and baby food as well as pregnant and lactating The purpose of this study was to optimizing the used of lipased papaya latex (Carica papaya latex) in the product to incorporation fatty acids rich in omega-3.Bioreactor acidolysis enzematik development and Optimization of process the productivity of fatty acids rich in omega-3. Reaction Acidolysis was conducted between tuna (thunnus thynnus) fish oil by microbial lipase (5% of the weight of the substrate mixture) or vegetable lipased (Carica papaya latex) (6-10% by weight subtract mixture) as biocatalist.Acidolysis mixture the bioreactor at 40 o C for (2-6 hours) and the stirring speed of 200 rpm, pH (4.5 to 6.5). to eliminate free fatty acids from the product acidolysis performed neutralization with NaOH The results are expected to show that the lipase papaya latex (Carica papaya latex) can be used as biocatalyst incorporated omega-3 fatty acids in   tunaIthunnus thunnyl) fish oil . In this study sought conditions for optimum incorporation rate, ie the use of papaya latex lipase concentration of 8%, the ratio of concentrations of omega-3 fatty acid and palm oil (1:1), pH = 5.5, Optimal time of 4 hours and the temperature (40oC) . The results were analyzed incorporation of omega-3 (EPA and DHA) with GC.EPA generated: 3.57% and DHA = 3.91% Key word:Acidolysis,Tuna fish oil