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All Journal Biogenesis: Jurnal Ilmiah Biologi Journal of Agromedicine and Medical Sciences Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Matematika Dan Ilmu Pengetahuan Alam LLDikti Wilayah 1 (JUMPA) Journal Community Service Consortium Hexagon Jurnal Agroindustri Halal
Meiria Istiana Sari
Program studi DIII Analis Farmasi Dan Makanan Akade
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Mechanical Engineering Neuroscience Physics Public Health

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PENGEMBANGAN DESA BEJI SEBAGAI WISATA EDUKASI KAMPUNG TEMPE Meiria Istiana Sari; Ambar Fidyasari; Kiantoro Andiek Setiawan
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 2 No. 3 (2021): Volume 2 Nomor 3 Tahun 2021
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v2i3.2806

Abstract

Desa Beji salah satu desa di Kota Batu yang terkenal dengan pengrajin tempe. Desa ini menjadi kampung terbesar tempe kedua di Malang raya setelah Sanan. Tempe merupakan salah satu warisan budaya tak benda yang diajukan ke UNESCO. Keunggulan ini kurang ditonjolkan sehingga perlu dilakukan pendampingan serta pengembangan di desa Beji yang potensinya di kota Batu sangat menjanjikan. Batu yang kerap dengan kota wisata menjadi pusat para wisatawan baik domestic maupun mancanegara. Sehingga dengan menggali potensi di Beji maka dapat mengenalkan bagaimana desa ini berkembang menjadi pusat wisata edukasi tempe. Metode pengabdian dilakukan melalui transfer keilmuan ke masyarakat dan pengrajin tempe, khususnya kelompok wanita Tani Asri, dikemas dalam penyampaian materi secara klasikal, workshop dan praktik pengenalan wisata edukasi tempe, pendampingan entrepreneur dan evaluasi. Kegiatan pengabdian masyarakat ini memiliki beberapa tahapan dimulai dari identifikasi permasalahan mitra perlunya edukasi pentingnya wisata tempe bagi Desa dan pembekalan transfer keilmuan tentang tempe. Hal ini terlihat dari hasil survei terhadap pentingnya wisata edukasi di desa ini yang menunjukkan 90%, pendampingan dari hasil entrepreneur 85%, promo diadakanya Festival Beji Kampung Tempe 97%, sehingga didapatkan hasil kepuasan keseluruhan sebesar 87%.
The LC-MS/MS Profile and Total Phenolic Content of Telang Flower Extract and Roselle as NPC1L1 Inhibitor Candidate Erna Susanti; Meiria Istiana Sari
Journal of Agromedicine and Medical Sciences Vol 8 No 3 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i3.32080

Abstract

Inhibition of atherogenesis can be done through several mechanisms, one of which is inhibiting intestinal cholesterol absorption through inhibition of NPC1L1. The activity of Telang flower extract in overcoming various problems of degenerative diseases, especially cardiovascular disease, has been proven by many scientific studies. Likewise, related to the activity of Rosella flowers. Previous research conducted by researchers showed the potential of Rosella flower extract as an LXR agonist, one of the pathways that play a role in NPC1L1 inhibition. The combination of the two extracts is expected to increase its activity as a candidate for NPC1L1 inhibitor. The purpose of this study was based on the results of the determination of total phenolic levels and the characterization of the active compounds with LC MS/MS extracts of telang flower and rosella, which are expected to be developed into further research in vivo to strengthen scientific evidence so that they can be developed into phytopharmaca preparations. Instrumentation analysis method with LC-MS/MS to identify the content of active compounds and determination of total phenolic content with Folin ciocalteu in order to obtain standardized extracts of telang flower and roselle. The results of this study showed that the total phenolic content based on the largest order was purple rosella flower extract (114.129.35 ± 266.20 g GAE), a combination of purple rosella and telang flower extract (101.760.58 ± 116.09 g GAE), telang flower extract (90.477.41 ± 107.38 g GAE) and extract red rosella flowers (84,601.00 ± 266.91 g GAE). The combination of extracts showed reduced total phenolic content compared to the single extract. The phenolic content in each extract affected the total phenolic in the combination of extracts. 8 anthocinin compounds were identified in both the telang flower extract and the rosella flower extract. Keywords: NPC1L1 inhibitor, telang, extract, Roselle, total phenolics, LC-MS/MS, atherogenesis
KARAKTERISASI PEKTIN EKSTRAK BONGGOL PISANG KEPOK (Musa paradisiaca L.) Andini Andini; Fadli Muhammad; Meiria Istiana Sari; Sentot Joko Raharjo
Hexagon Jurnal Teknik dan Sains Vol 3 No 2 (2022): HEXAGON - Edisi 6
Publisher : Fakultas Teknologi Lingkungan dan Mineral - Universitas Teknologi Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.858 KB) | DOI: 10.36761/hexagon.v3i2.1626

Abstract

Pisang dapat ditemukan di semua di daerah tropis di Indonesia dan memiliki berbagai jenis. Tidak hanya buahnya tapi bonggol pisang juga memiliki kandungan yang bermanfaat yaitu pektin. Pektin merupakan golongan polisakarida pada dinding sel tumbuhan yang mengandung asam galakturonat yang salah satu fungsinya adalah sebagai pengawet. Penelitian ini bertujuan untuk mengekstraksi pektin dari bonggol pisang dan kemudian dilakukan karakterisasi untuk menentukan mutu pektin. Penelitian ini dilakukan dengan metode ekstraksi menggunakan pelarut HCl kemudian ditambahkan etanol ke dalam filtrat untuk mengendapkan pektin dan kemudian dilakukan pengeringan untuk mengendapkan pektin kering. pektin kering kemudian dilakukan karakterisasi dengan beberapa parameter, diantaranya rendemen, bobot pektin, kadar air, kadar abu, berat ekivalen, kadar metoksil, kadar asam galakturonat, dan derajat esterifikasi. Hasil dari pengujian didapatkan data rendemen sebesar 19,6546%; kadar air sebesar 8,19%; kadar abu sebesar 0,3494%; berat ekivalen sebesar 625,211; kadar metoksil sebesar 3,27%; kadar asam galakturonat sebesar 321,94%; dan derajat esterifikasi sebesar 16,89%.
Analysis and identification of flavonoid compounds in kepok banana corm extract (musa paradisiaca L) Andini, Andini; Sari, Meiria Istiana; Raharjo, Sentot Joko; Anneke, Anneke
Jurnal Matematika Dan Ilmu Pengetahuan Alam LLDikti Wilayah 1 (JUMPA) Vol. 4 No. 1 (2024): Marc: Mathematics and natural science
Publisher : Lembaga Layanan Pendidikan Tinggi Wilayah I Sumatra Utara (LLDikti I)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54076/jumpa.v4i1.300

Abstract

Kepok banana corm is one of the natural ingredients that has been obtained and can provide many benefits because of its excessive flavonoid content. This study aims to determine the types of flavonoids contained in the kepok banana weevil extract. Methods of detection and discovery of flavonoid measurement methods used include reaction, thin layer chromatography, and LC-MS/MS, and spectrofotometri for total flavonoid content compared to rutin as a flavonoid standard. The results showed that kepok banana corm extract contains a class of flavonoid compounds including rutin, kaempferol 3-O-rhamnoside-7-O-glucoside, quercetin 3 glycocide, and chrysoeriol-7-O-glycuronyl, with a total flavonoid content of 6.19. ±0.65%.
EDUKASI DAN PENDAMPINGAN PENGOLAHAN PASCA-PANEN LIDAH BUAYA SEBAGAI UPAYA PEMULIHAN EKONOMI MASYARAKAT TERDAMPAK PANDEMI DI KAMPUNG LIDAH BUAYA, RW 11, DESA BANJAR ARUM, SINGOSARI, MALANG Raharjo, Sentot Joko; Andini, Andini; Sari, Meiria Istiana; Setiawan, Kiantoro Andiek
Journal Community Service Consortium Vol 3 No 1 (2023): Journal Community Service Consortium
Publisher : Universitas Ciputra Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/consortium.v3i1.3650

Abstract

Lidah buaya merupakan salah satu tanaman yang dapat digunakan sebagai produk pangan nabati maupun kosmetik. Di Kampung Lidah Buaya Desa Banjararum, Kecamatan Singosari, Kabu- paten Malang merupakan salah satu tempat pusat pengolahan produk lidah buaya. Di Kampung Lidah Buaya selama ini telah dikembangkan produk pangan bersumber dari lidah buaya. Salah satu produk pengembangan pengolahan lidah buaya yang lain dapat digunakan sebagai bahan kosmetik lotion, namun kendala yang dihadapi pengrajin belum mampu mengembangkan teknologinya dan menerapkan Good Manufacturing Practices (GMP) agar produk yang dihasilkan nantinya konstan. Adanya potensi kampung lidah buaya tersebut maka dilakukan program edukasi dan pendampingan kepada para pengrajin pengolahan lidah buaya. Tujuan utama dalam program pendampingan ini adalah meningkatkan pemahaman dan pengetahuan kepada pengrajin pengolahan lidah buaya menjadi produk kosmetik lotion yang berkualitas dan penerapan Good Manufacturing Practices (GMP) bagi produk agar dapat memperluas pasar usahanya. Adapun mitra sasaran program pendampingan masya- rakat ini adalah kelompok Pengrajin Pengolahan Lidah Buaya di Kampung Lidah Buaya, Desa Banjar- arum, Kabupaten Malang yang telah produktif secara ekonomi sebagai pengrajin pengolahan produk lidah bersumber dari tanaman lidah buaya. Adapun usaha yang akan dilakukan adalah edukasi kepada pengrajin ini tentang ilmu dan teknologi sediaan gel lotion lidah buaya dan beberapa penerapan GMP-nya. Edukasi ini diharapkan adanya penerapan iptek sediaan lotion lidah buaya dan perubahan perilaku pengrajin dalam proses produksi. Metode yang digunakan adalah Participatory Action Research (PAR) berupa penyuluhan, demonstrasi, serta pendampingan. Pendampingan dilakukan selama 1 bulan. Hasil edukasi dan pendampingan menunjukkan bahwa 95,0% responden setuju bahwa kegiatan pengabdian masyarakat bermanfaat, 89,0%, responden setuju kegiatan edukasi ini sangat meningkatkan kualitas, 97.0% responden menyatakan ilmu bertambah setelah mengikuti pelatihan, dan 85,0% responden menyatakan bahwa keseluruhan kegiatan pelatihan sudah baik.
Studi In Vitro dan In Silico pada Ekstrak Bonggol Pisang Kepok sebagai Anti-tirosinase Andini, Andini; Raharjo, Sentot Joko; Misgiati, Misgiati; Izza, Aqidatul; Istiana, Meiria
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.53561

Abstract

Tyrosinase is a critical enzyme in melanin synthesis, where its overactivity can result in hyperpigmentation disorders. Despite the availability of synthetic tyrosinase inhibitors, concerns about safety have prompted the search for natural alternatives. The objective of this study is to evaluate the potential of Kepok banana (Musa acuminata x balbisiana) corm extract as a natural tyrosinase inhibitor, using in vitro and in silico approaches. The extract was prepared by maceration with etanol 96%, followed by sonication and lyophilization. LC-MS analysis was performed on a Shimadzu LCMS 8040 using a Shim Pack FC ODS column with 90% methanol as the mobile phase under isocratic conditions. Samples were ionized via ESI in positive mode and scanned over m/z 10–1000. Peaks were identified based on their m/z values and MS/MS fragmentation patterns. In vitro and in silico analyses were applied, respectively, to study the biological process of kepok banana corm extract as a natural tyrosinase inhibitor. The result of the kepok banana corm extraction process was 12.5% dry powder with bioactive components identified via LC-MS, including flavonoids, phenolics, and terpenoids. The kepok banana corm ethanol extract exhibited strong tyrosinase inhibition with an IC₅₀ of 91.074 µg/mL, falling below the 100 µg/mL threshold for strong inhibitors and showing a similar inhibition pattern to the standard kojic acid (IC₅₀ = 10.87 µg/mL). Molecular docking revealed that major compounds, particularly flavonoids, exhibited high binding affinity with the tyrosinase enzyme through hydrogen bonding and hydrophobic interactions, similar to kojic acid, a known inhibitor. These findings suggest that kepok banana corm extract may serve as a promising natural tyrosinase inhibitor, with potential applications in hyperpigmentation treatment and cosmetic formulations.
ANALISIS RHODAMIN B PADA MAKANAN JAJANAN ANAK DI SEKITAR SDN SRATUREJO 1 DAN SDN SRATUREJO 2 DI DESA SRATUREJO KECAMATAN BAURENO KABUPATEN BOJONEGORO Andini Andini; Novia Eka Wijayanti; Meiria istiana Sari; Anggraeni In Oktavia In Oktavia
Hexagon Vol 4 No 2 (2023): HEXAGON - Edisi 8
Publisher : Fakultas Teknologi Lingkungan dan Mineral - Universitas Teknologi Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36761/hexagon.v4i2.3133

Abstract

Rhodamin B merupakan zat warna sintetik yang digunakan sebagai pewarna tekstil dan dilarang dalam penggunaannya, bila diperhatikan pada makanan yang mengandung Rhodamin B warna merah mencolok yang menggumpal dan tidak rata pada makanan. Rhodamin B jika dikonsumsi bisa menyebabkan pembesaran hati, ginjal, dan limfa serta pembesaran organ. Analisis Rhodamin B adalah analisis yang dilakukan terhadap pewarna sintetik jenis Rhodamin B yang digunakan untuk pewarna tekstil dimana penggunaannya sengaja ditambahkan pada makanan yang menimbulkan bahaya kesehatan bagi manusia. Penelitian ini bertujuan untuk mengetahui ada tidaknya pewarna Rhodamin B pada jajanan anak terutama pada jajanan Kerupuk, Kembang Gula, Jeli dan untuk mengetahui kadar Rhodamin B dari sampel makanan jajanan anak di sekitar SDN Sraturejo 1 dan SDN Sraturejo 2. Penelitian ini dilakukan 2 tahap, yaitu secara kualitatif dan kuantitatif. Data penelitian di analisa dengan menggunakan metode KLT dengan replikasi 3 kali, apabila terdapat warna noda yang sama dan harga Rf yang mendekati dengan standar maka akan dilanjutkan dengan menggunakan metode Spektofotometer dengan panjang gelombang 558nm. Berdasarkan hasil analisis didapatkan hasil persamaan regresi linier y= a+bx (y = 1,938 + 0,205x) dengan kadar Rhodamin B 0,075±0,0027 dari masing masing sampel.
IDENTIFIKASI RHODAMIN B DAN METHANIL YELLOW PADA JAJANAN ANAK DI SEKOLAH DASAR DENGAN METODE KROMATOGRAFI LAPIS TIPIS Meiria Istiana Sari; Andini
Hexagon Vol 4 No 2 (2023): HEXAGON - Edisi 8
Publisher : Fakultas Teknologi Lingkungan dan Mineral - Universitas Teknologi Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36761/hexagon.v4i2.3149

Abstract

Rhodamine B is a synthetic dye whose use is prohibited in cosmetics and declared a hazardous substance according to the Regulation of the Minister of Health (PerMenKes) No.239/MenKes/Per/V/1985 because it can cause liver, kidney and spleen damage followed by anatomical changes in the form of enlargement organ. Rhodamine B is often abused in food, beverage, drug and cosmetic products. This study aims to find out whether the children's snacks made from cotton candy and sand crackers contain rhodamine B. This study used a qualitative test, namely the Thin Layer Chromatography method. The principle is the absorption of the color of the woolen thread samples in an acidic environment by heating, followed by dissolving the colored woolen threads. Each sample was replicated 3 times and then compared with standard rhodamine B using eluent n-butanol : glacial acetic acid : water (4 : 5 : 1). The results showed that the 8 samples tested did not identify the presence of rhodamine B dyes. The conclusion of this study was that 8 samples of confectionery and sand crackers did not contain rhodamine B
The Analysis of Benzoate in Deepika Sauce of Sejahtera Sentosa SME with Uv-Vis Spectrophotometry Method Andini Andini; Meiria Istiana Sari; Mokh. Suseno Aji Sari
JURNAL AGROINDUSTRI HALAL Vol. 10 No. 3 (2024): Jurnal Agroindustri Halal
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jah.v10i3.11920

Abstract

The food additives level of use, particularly benzoate as preservatives, must comply with Indonesian regulations to ensure food safety for consumers. This study is aimed to determine the benzoic compounds content and determine the levels of benzoic compounds in 6 types of sauce manufactured by Sejahtera Sentosa SME. The identification of benzoate compounds was carried out via FeCl3 which was characterized by the formation of a brown precipitate while determination of the benzoate compounds level was conducted via the UV-Vis spectrophotometry method at a wavelength of 274 nm. The results indicated that the six samples of Deepika Sauce A, B, C, D, E and F contained benzoate preservatives sequentially by 0.986 ; 0.943 ; 0.852 ; 0.635 ; 0.700 ; and 0.992 g/kg. The benzoate preservatives use in chili sauce samples does not exceed the threshold set in the Regulation of the Head of the Drug and Food Control Agency of the Republic of Indonesia Number 11 of 2019, namely 1 g/kg.
Co-Authors Ambar Fidyasari Andini Andini Andini Andini Andini, Andini Anggraeni In Oktavia In Oktavia Anneke, Anneke Erna Susanti Fadli Muhammad izza, Aqidatul Kiantoro Andiek Setiawan Misgiati, Misgiati Mokh. Suseno Aji Sari Novia Eka Wijayanti Sentot Joko Raharjo