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All Journal Alchemy Jurnal Penelitian Kimia Biosaintifika: Journal of Biology & Biology Education
Awan Purnawan
Research Center for Biotechnology, Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Energy Environmental Science Materials Science & Nanotechnology

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IDENTIFIKASI SENYAWA KIMIA PADA TUMBUHAN OBAT (Calophyllum sepctabile WILD) Purnawan, Awan
Alchemy Jurnal Penelitian Kimia Vol 2, No 2 (2003)
Publisher : Alchemy Jurnal Penelitian Kimia

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Abstract

Isolasi dan identifikasi senyawa kimia yang ada pada kulit kayu calophyllum spectabile Wild telah dilakukan dengan menggunakan pel;arut methanol panas. Filtrat depekatkan dengan penguap bertekanan rendah hingga diperoleh ekstrak kering. Ekstrak dipartisi dengan menggunakan campuran etilasetat – air. Ekstrak etil asetat dimurnikan dengan pemisahan kolom kromatografi dengan pelarut n-heksana-etilasetat. Hasil ekstraksi,fraksionisasi, pemurnian senyawa dianalisis dan diidentifikasi oleh spektrometri Ultra Lembayung, Spektrometri Infra Merah, Spektrofotometri Massa, menunjukkan adanya senyawa dengan gugus fungsi karbonil, keton metal, metilen, dan senyawa dengan berat molekul 427 yang disebut friedelin.
Production of Manooligomannan from Palm Kernel Cake by Mannanase Produced from Streptomyces Cyaenus Purnawan, Awan; Yopi, Yopi; Irawadi, Tun Tedja
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.9201

Abstract

The increase of public attention to health has prompted researchers to look for new sources of functional food. Palm Cake Kernel (PKC) waste was abundant in Indonesia, Oligosaccharide has an important benefit for human health. Recently oligosaccharide is not only important as an artificial sweetener, but also as a functional food component. This study was aimed to produce oligo-mannan enzymatically from PKC waste using mannanase derived from of Streptomyces cyaenus isolates of indigenous Indonesia. The enzyme concentration was determined by enzyme activity assay while oligo-mannan content in the PKC was analyzed using TLC and HPLC. Mannanase enzyme activity of 1706 U/ml on the second day of agitation 200 rpm at a temperature of 30C Hydrolysis of mannooligomannan by using mannanase produced by streptomyces cyaenus. The optimum mannanase enzyme activity obtained on day 2 with the value of the activity as much of 0.702 U/mL. The protein content of the 2nd day at an agitation speed of 150 rpm, 200 rpm, and 250 rpm, respectively, were 1783, 1950 and 2283 ppm. Streptomyces cyaenus is Indonesian original isolates potentially producing mannanase that can produce mannooligomannan.
Co-Authors Tun Tedja Irawadi YOPI YOPI