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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pijar MIPA
Erin Ryantin Gunawan
University of Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Mathematics Physics

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Optimasi Sintesis Asam Lemak Etanolamida Berbasis Minyak Ketapang Menggunakan Response Surface Methodology Lilik Indriani; Erin Ryantin Gunawan; Dedy Suhendra
Jurnal Pijar Mipa Vol. 16 No. 2 (2021): Maret 2021
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.812 KB) | DOI: 10.29303/jpm.v16i2.2265

Abstract

Telah dilakukan optimasi sintesis asam lemak etanolamida berbasis minyak ketapang (Terminalia catappa L) menggunakan Response Surface Methodology (RSM). Asam lemak etanolamida adalah surfaktan yang banyak dibutuhkan pada industri farmasi. Sintesis asam lemak etanolamida dilakukan dengan mereaksikan minyak ketapang dan etanolamina sebagai substrat menggunakan katalis Lipozyme TL.IM. Penelitian ini bertujuan untuk mengamati interaksi antar variabel yang dapat mempengaruhi kondisi optimum dan hasil reaksi. Beberapa variabel yang dimaksud yaitu waktu reaksi, suhu reaksi, molar rasio substrat dan jumlah enzim. Metode RSM yang digunakan terdapat dalam perangkat lunak Design Expert v.7. Hasil penelitian menunjukkan model untuk mendapatkan kondisi yang optimal adalah model quadratic (p-value 0.0048/signifikan), lack of fit (p-value 0.3827 /non signifikan) dan nilai R-squared sebesar 0,7953. Kondisi optimum yang dihasilkan yaitu waktu reaksi 1,50 jam, suhu reaksi 38,10 °C, molar ratio minyak : etanolamina (1 : 20,52) gram/mol dan jumlah enzim 0.16 gram dengan prediksi dan aktual persen hasil asam lemak etanolamida secara berturut-turut sebesar 60,02 dan 64,00 %.
Isolation of Lipase from Candlenut Seeds and Its Immobilization in Polyacrylamide Gel Zella Yaumin Nasry; Nyoman Maya Krisnawati; Erin Ryantin Gunawan; Jannatin ‘Ardhuha; Agus Abhi Purwoko; Lalu Rudyat Telly Savalas
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 3 (2022): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i3.1483

Abstract

In general, lipase enzymes are used in the form of free lipase enzymes which can only be used once and are less effective, so that their use is costly. Therefore, to maximize the use of the lipase enzyme, immobilization of the enzyme that allows multiple use is a choice. The purpose of this study was to determine the activity of the candlenut (Aleurites moluccana (L.) Wild) lipase in its free form and in immobilization form. The stability of the immobilized lipase enzyme with repeated use was also investigated. The enzyme was immobilized by trapping method using polyacrylamide gel. The immobilized lipase enzyme was characterized to determine its stability after repeated use with various gel concentrations. The crude extract of the lipase of candlenut seed sprouts had an activity of 2.32 U/mL, whereas the activity of the immobilized lipase enzyme in polyacrylamide gel at concentrations of 6%, 8%, and 10%, were 2.133; 2,259; and 2,311 U/mL, respectively. The optimum temperature for the immobilized lipase enzyme was 30 oC and the optimum pH was 7.5. After five time us usage, the immobilized enzyme showed activities of 49.49%, 72.60% and 86.53%, for 6%, 8% and 10% polyacrylamide gel, respectively. These results indicate that the immobilized lipase enzyme from seed sprouts of candlenut (Aleurites moluccana (L.) Wild) by trapping method using 10% polyacrylamide gel can maintain its stability after five times of use
Utilization Of Moringa Seed Oil Fatty Acids for Azelaic Acid Base Stock Production Sri Seno Handayani; Erin Ryantin Gunawan; Dedy Suhendra; Murniati Murniati; Dhony Hermanto; Liliek Karnila
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 5 (2022): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i5.1964

Abstract

Moringa (Moringa oleifera L.) is a plant belonging to the moringaceae family. Moringa seeds contain oil which can be used as a raw material for making azelaic acid. Azelaic acid is used as an anti-acne in the pharmaceutical and cosmetic industries. Azelaic acid can be obtained from the oxidation process of fatty acids in moringa seed oil using potassium permanganate as an oxidizing agent. The resulting azelaic acid is in the form of white crystals with a conversion percentage of 72.17%. The results of the FT-IR characterization showed the formation of azelaic acid characterized by widening of the hydroxyl group at 3448.46 cm-1 followed by a shift of the ester group at absorption of 1633.46 cm-1. The ester bond is strengthened at a wavelength of 1115.95 cm-1.
Co-Authors Agus Abhi Purwoko Dedy Suhendra Dhony Hermanto Jannatin ‘Ardhuha Lalu Rudyat Telly Savalas Liliek Karnila Lilik Indriani Murniati Murniati Nyoman Maya Krisnawati Sri Seno Handayani Zella Yaumin Nasry