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All Journal Biosaintifika: Journal of Biology & Biology Education Berkala Penelitian Hayati
Ari Indrianto
Faculty of Biology, Gadjah Mada University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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IN VITRO CULTURE OF ORCHIDS: THE ROLES OF CLASS-1 KNOX GENE IN SHOOT DEVELOPMENT Endang Semiarti; Aziz Purwantoro; Ari Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 1 (2014): December 2014
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.385 KB) | DOI: 10.23869/114

Abstract

In vitro culture of orchids has been developed for many purposes. Some native orchids and commercial orchid hybrids are propagated using seed germination or cut explants such as leaves, shoot tips, and roots to produce large numbers of orchid plantlets. This technique is widely used for the purpose in conservation of natural orchid species and industry of commercial orchid hybrids. However, the molecular genetic mechanism behind growth and development of these orchids during in vitro culture is still unclear, and needs to be elaborated. Recent advanced in transgenic technology in orchid is very helpful for studying the mechanism of action of key genes in various stages of orchid development during in vitro culture. In this review, an attempt to understand the role of class-1 KNOX gene and its relationship with other genes in the initiation of shoot apical meristem (SAM) for shoot development from orchid protocorm (a tubercle of developing orchid embryo) and PLBs (Protocorm Like Bodies) during in vitro culture will be discussed. It will answer the question about how the shoot formation can be controlled during growth and development of orchid cells in in vitro culture.
Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol Noor Aini Habibah; Sukarti Moeljopawiro; Kumala Dewi; Ari Indrianto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6632

Abstract

Stelechocarpus burahol is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro S. burahol from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration of plant growth regulators i.e. picloram (5, 7.5 and 10 mg/L) and 2, 4-D (10, 15 and 20 mg/L) under dark condition. Induction of callus formation started on the 20.29th to the 29.86th days. Medium supplemented with Picloram and dark state proved to be the best condition for optimum callus induction from mesocarp explants of S. burahol. Callus grown on medium with the addition of 7.5 mg/l Picloram produces the highest flavonoid. The maximum production of the secondary metabolite was obtained from 8 weeks old callus. However, by the time of callus ageing, its output has declined. It could be concluded that callus cultures from mesocarp S. burahol can be used for flavonoid production. How to CiteHabibah, N. A., Moeljopawiro, S. Dewi, K. Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol. Biosaintifika: Journal of Biology Biology Education, 8(2), 214-221.
Co-Authors Aziz Purwantoro Endang Semiarti Kumala Dewi Noor Aini Habibah Sukarti Moeljopawiro