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All Journal Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Biosaintifika: Journal of Biology & Biology Education Berkala Penelitian Hayati
Bambang Irawan
Biologi, FMIPA, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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AMBANG WAKTU PENDEDAHAN TELUR Lymnaea sp UNTUK MENGAMATI ABNORMALITAS EMBRIO SEBAGAI PARAMETER DALAM UJI HAYATI TERHADAP KARBAMAT Hari Soepriandono; Bambang Irawan; Sri Puji Astuti Wahyuningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 1 (2000): December 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/488

Abstract

The aim of this study was to know the specific expose period of the Lymnaea sp.’s embyos and their age in order to utilization eggs of Lymnaea sp. in bioassay. Fifty individuals of Lymnaea sp. collected from Gonggan River Magetan were captured in laboratory. Egg cluster of each individual then was placed in petry dish which was filled by Baycarb 500 EC of 0.0001, 0.001 and 0.01 ppm. The result showed that 12 hours exposure in Baycarb 500 EC was the effective time since within 6 hours all of embryos were death and abnormalities of the survivals was detected in the embryos of 48 and 72 hours.
ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI Escherichia coli DH5a Ni Nyoman Tri Puspaningsih; Akhmaloka; Sofyan Hadi; Y. Sri Wulan Manuhara; Bambang Irawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 4 No 2 (1999): June 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/517

Abstract

Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert. Characterization of the recombinant DNA by Stu1 and EcoR1 restriction enzymes indicated nucleotide sequence of insert gene. Digestion by BamHI, Cla1, and EcoRV restriction enzymes showed only one band that assumed size of insert gene is about 1500 bp. Gene expression showed that amylase enzyme activity by using Somogyi-Nelson method was 88.0265 Unit. This activity was 10% higher than transformant control (Escherichia coli DH5a which content YCp50).
DNA Barcoding: A Study of Guppy Fish (Poecilia reticulata) in East Java, Indonesia Sucipto Hariyanto; Hasan Adro’i; Mahrus Ali; Bambang Irawan
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.20222

Abstract

Poecilia reticulata is a freshwater fish from the northeastern part of South America and spread widely to various countries in Asia and other continents. However, research about P. reticulate is limited even though it is a well-known fish species in Indonesia. The purpose of study was to identify the fish species of P. reticulata through DNA barcoding using the COI gene to determine the phylogenetic relationships among fish populations in East Java, Indonesia. In a present study, there were eight samples of P. reticulata from four different freshwater locations in East Java. Extraction, amplification, and sequencing of DNA samples were conducted to obtain the genetic data and construct a phylogenetic tree based on DNA sequences. The COI gene is the most popular markers to study genetic populations and phylogeography among the animal kingdom. Our phylogenetic reconstruction showed a clear that there were two groups of P. reticulata. The first group was obtain through species from East Java, Sukabumi, West Java (KU692776.1), Dominican Republic, Pandeglang, Banten and Myanmar. The second group was P. reticulata from southern Africa, Brazil, and Sukabumi, West Java (KU692775.1). The result of this study indicate that the guppy fish in East Java identic with P. reticulata from West Java (KU692776.1), which a widely used in classification based on evolutionary relationships. The findings of this study have important implication for the development of advance research about adaptation, phylogeny, and evolution of fish, especially of guppy fish.
Co-Authors Acivrida Mega Charisma Affandi, Moch Agoes Soegianto Akhmaloka Astra Budi Priatama Clara Puspita Hani Sudarmanto Hani Sudarmanto Hari Soepriandono Hasan Adro’i Hening Swastikaningrum Indinah Indinah Indinah Leonard Ady Candra Mahrus Ali Moch. Syaifullah Ni Nyoman Tri Puspaningsih Noer Moehammadi Noer Moehammadi Noorafebrianie Minarputri Ragil Siti Rihadini Sofyan Hadi Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sucipto Hariyanto Thin Soedarti Y. Sri Wulan Manuhara Yuniar Ade Candra