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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) IPTEK Journal of Proceedings Series Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Biologi dan Pembelajarannya (JBP) UNEJ e-Proceeding Indonesian Journal of Chemistry Jurnal Perikanan Universitas Gadjah Mada Jurnal Purifikasi Jurnal Medik Veteriner Aquacultura Indonesiana Berkala Penelitian Hayati Journal Of Sustainability Perspectives Jurnal Ilmiah Sains Journal of Sustainability Perspectives Journal of Fisheries & Marine

Agoes Soegianto

Department Of Biology, Faculty Of Science And Technology, Airlangga University

Author-ID : 383640

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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Biomonitoring Escherichia coli and Coliform Contamination in Abalone (Haliotis squamata) Cultivation Pond in Musi Village, Gerokgak Sub-District, Buleleng-Bali. Wiradana, Putu Angga; Yusup, Deny Suhernawan; Soegianto, Agoes
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Abalone (Haliotis squamata) is one of the fisheries commodities that have high economic value. This study was conducted to investigate contamination of Escherichia coli and coliform in Abalone cultivation ponds in the Musi Village area, Buleleng Regency, Bali. An experiment was carried out by taking water samples in the abalone pond every week for one month. The results showed that there was no difference between the total number of Escherichia coli and coliform. Water samples that were positive for coliform in the determination test had no differences in each sample with total coliform values (Colonies / 100mL) of 4, 3, 7 and 9 (MPN / 100mL) respectively. While the total number of Escherichia coli is shown after being grown in EMBA selective media which are 4, 3, 7, 4 (MPN / 100mL) respectively. Meanwhile, when compared with the number referenced from the Decree of the State Minister of Environment Number 51 of 2004 concerning Sea Water Quality Standards, the quality of seawater for aquatic cultivation in this study is still classified as Safe (<1000 MPN / 100mL).

Daya Dukung dan Laju Pertumbuhan Microcystis Hasil Isolasi dari Waduk Sutami pada Berbagai Variasi Konsentrasi Nitrat dan Fosfat dalam Medium Selektif B-12 Retnaningdyah, Catur; Suharjono, Suharjono; Soegianto, Agoes; Irawan, Bambang
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

The main objective of this research was to calculate the carrying capacity and growth rate ofisolated Microcystis result in Sutami reservoir on a variety of nitrate and phosphateconcentrations in the B-12 selective medium. Research was conducted in the laboratory withpure experiments using completely randomized factorial design with factors of nitrateconcentration variation (8, 16, 32, and 64 ppm) and phosphate (0.2, 0.4, 0.8 and 1.6 ppm) in B-12medium. Repetition of the study was conducted three times at the same time. Microcystispopulation abundance which was counted every day until the stationary phase (day +30) wasused to calculate the rate of growth (Î²) and maximum abundance of Microcystis can besupported by each medium treatment (Î³). The results showed that the growth rate of Microcystiswas not significantly influenced by levels of phosphate in the medium but significantly positivelycorrelated with increasing nitrate concentration in the medium. Carrying capacity or themaximum abundance (Î³) of Microcystis was influenced by the combination of nitrate andphosphate in the B12 medium. Concentration of phosphate 0.4 ppm in medium combined withnitrate 8âˆ’64 ppm could support the highest abundance of Microcystis.

Blooming Stimulation of Microcystis in Sutami Reservoir Using Nutrients Nitrate and Phosphate in Different ratio Retnaningdyah, Catur; Suharjono, Suharjono; Soegianto, Agoes; Irawan, Bambang
Journal of Tropical Life Science Vol 1, No 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Microalgae Cyanobacteria from the genus Microcystis has the potential to bloom in Sutami reservoirs that located in Malang, Indonesia at a particular time. Microcystis is a notorious species because it can produce toxins that are dangerous to other organisms. The objective of this research is to determine the influence of different ratios of Nitrate and Phosphate on the growth of Microcystis in Sutami reservoir. The study was conducted from April to November 2009 and was carried out in situ in the reservoir Sutami with quasi experiment using a completely randomized design. Sutami reservoir water which already contained plankton community therein, including Microcystis were treated with five variations ratio of Nitrate and Phosphate (10, 20, 40, 80, and 160) and untreated water as control. Experiments carried out by using a transparent plastic bag diameter 1 m and 1.5 m long, inserted into the water and be immersed in the water by using bamboo. Calculation of Microcystis abundance and physic-chemical quality of water is done every three days for a month. The results showed that the higher concentration of orthophosphate in the media water of Sutami reservoir have the maximum abundance of Microcystis. Abundance of Microcystis grown in Sutami reservoirs have positively correlated with actual levels of nitrate, nitrite, total phosphate, conductivity, pH, temperature, ratio of Nitrate to Phosphate treatment and water KMnO4 value.Keywords: Blooming Microcystis, the ratio of nutrients Nitrate to Phosphate, Sutami reservoir

Pattern of Biogas Production and Removal of Chemical Oxygen Demand in Semi Continues Hybrid Anaerobic Reactor Oktavitri, Nur Indradewi; Soegianto, Agoes; Rahman, T.; Saadah, Bidayatus; Trianingtyas, Deavy
IPTEK Journal of Proceedings Series Vol 1, No 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2014i1.229

Anaerobic process had been used for treated highly organic substances, such as canteen wastewaterÂ which had COD around 5000 mg/L. This research aims to investigate pattern of biogas production and COD removal in semi continue anaerobic process. HRT (Hydraulic Retention Times) were used in this research 1,5 hours. The reactor was contained of four cell coulombs from PVC, with height 1 meter. Gravel as media support in hybrid anaerobic reactor. The height of media support is 50 cm. This reactor operated for 14 days. Parameters had been observed are biogas production and COD.. Biogas production after reactor operated 7 days 0,6594 mL and after reactor operated 14 days is 1,4758 mL. COD removals are 38,89 mg/L after operated 7 days and 42,86 mg/L after operated 14 days. In conclusion, COD removal increased after reactor operated 7 and 14 days. The biogas production increased after 7 and 14 operated days. Increasing of biogas production more drastically than COD removal

KANDUNGAN LOGAM NON ESENSIAL (Pb, Cd dan Hg) DAN LOGAM ESENSIAL (Cu, Cr dan Zn) PADA SEDIMEN DI PERAIRAN TUBAN GRESIK DAN SAMPANG JAWA TIMUR Moch. Syaifullah; Yuniar Ade Candra; Agoes Soegianto; Bambang Irawan
Jurnal Kelautan Vol 11, No 1 (2018)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v11i1.4497

Logam berat seperti Pb, Cd, Hg, Cu, Cr dan Zn diperairan akan turun kemudian mengendap membentuk sedimen, beberapa organisme yang mencari makan didasar perairan akan mempunyai peluang besar untuk terpapar logam berat ini. Penelitian ini bertujuan untuk mengetahui kandungan logam berat non esensial Pb, Cd, Hg dan logam berat esensial Cu, Cr, Zn pada sedimen di perairan Tuban, Gresik dan Sampang. Kandungan logam berat pada sedimen ini menggunakan Atomic Absorption Spectrometry (AAS). Hasil penelitian menunjukkan bahwa kandungan logam non esensial timbal (Pb) yakni 3,3687 ± 0,5257 mg/kg-1 di Gresik, 2,9443 ± 0,3608 mg/kg-1 di Tuban dan 3,4253 ± 0,3697 mg/kg-1 di Sampang. Kandungan Kadmium (Cd) 2,978 ± 0,224 mg kg-1 di Tuban, 2,955 ± 0,179 mg kg-1 di Gresik, 3,012 ± 0,148 mg kg-1 di Sampang. Kandungan merkuri (Hg) 0,000029 ± 0,000012 mg kg-1 di Tuban, 0,000033 ± 0,000012 mg kg-1 di Gresik, 0,000034 ± 0,000045 mg kg-1 di Sampang. Adapun hasil analisis Kandungan logam esensial. Kandungan Tembaga (Cu) 0,0780 ± 0,0069 mg/kg-1 di Tuban, 0,0740 ± 0,0118 mg/kg-1 di Gresik dan 0,0750 ± 0,0069 mg/kg-1 di Sampang. Kandungan Kromium (Cr) 0,000033 ± 0,0000045 mg/kg-1 di Tuban, 0,000034 ± 0,0000011 mg/kg-1, 0,000024 ± 0,000009 mg/kg-1 di Sampang. Kandungan seng (Zn) 6,983 ± 0,278 mg kg-1 di Tuban, 5,873 ± 0,233 mg kg-1 di Gresik, 5,172 ± 0,233 mg kg-1 di Sampang.Kata kunci: logam non esensial, logam esensial, sedimenNON-ESSENTIAL METAL CONTENT (PB, CD AND HG) AND ESSENTIAL METALS (CU, CR AND ZN) IN SEDIMENTS IN THE WATERS OF TUBAN GRESIK AND SAMPANG, EAST JAVAABSTRACTHeavy metals such as Pb, Cd, Hg, Cu, Cr and Zn in the water will drop then settle to form sediments, some organisms that feed on the basis of water will have a great chance of being exposed to this heavy metal. This study aims to determine the content of non-essential heavy metals Pb, Cd, Hg and essential heavy metals Cu, Cr, Zn in sediments in the waters of Tuban, Gresik and Sampang. The heavy metal content in this sediment uses Atomic Absorption Spectrometry (AAS). The results showed non-essential metal content of lead (Pb) was 3.3687 ± 0.5257 mg / kg-1 in Gresik, 2.9443 ± 0.3608 mg / kg-1 in Tuban and 3.4253 ± 0.3697 mg / kg-1 in Sampang. The content of Cadmium (Cd) is 2.978 ± 0.224 mg kg-1 in Tuban, 2.955 ± 0.179 mg kg-1 in Gresik, 3.012 ± 0.148 mg kg-1 in Sampang. The content of mercury (Hg) is 0.000029 ± 0.000012 mg kg-1 in Tuban, 0.000033 ± 0.000012 mg kg-1 in Gresik, 0,000034 ± 0,000045 mg kg-1 in Sampang. The results of the analysis of the content of essential metals. Copper (Cu) content 0.0780 ± 0.0069 mg / kg-1 in Tuban, 0.0740 ± 0.0118 mg / kg-1 in Gresik and 0.0750 ± 0.0069 mg / kg-1 in Sampang. Chromium content (Cr) 0,000033 ± 0,0000045 mg / kg-1 in Tuban, 0,000034 ± 0,0000011 mg / kg-1, 0,000024 ± 0,000009 mg / kg-1 in Sampang. The zinc content (Zn) is 6.983 ± 0.278 mg kg-1 in Tuban, 5.873 ± 0.233 mg kg-1 in Gresik, 5.172 ± 0.233 mg kg-1 in Sampang.Keywords: non-essential metals, essential metals, sediments

Daya Dukung dan Laju Pertumbuhan Microcystis Hasil Isolasi dari Waduk Sutami pada Berbagai Variasi Konsentrasi Nitrat dan Fosfat dalam Medium Selektif B-12 Catur Retnaningdyah; Suharjono Suharjono; Agoes Soegianto; Bambang Irawan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2590

The main objective of this research was to calculate the carrying capacity and growth rate ofisolated Microcystis result in Sutami reservoir on a variety of nitrate and phosphateconcentrations in the B-12 selective medium. Research was conducted in the laboratory withpure experiments using completely randomized factorial design with factors of nitrateconcentration variation (8, 16, 32, and 64 ppm) and phosphate (0.2, 0.4, 0.8 and 1.6 ppm) in B-12medium. Repetition of the study was conducted three times at the same time. Microcystispopulation abundance which was counted every day until the stationary phase (day +30) wasused to calculate the rate of growth (β) and maximum abundance of Microcystis can besupported by each medium treatment (γ). The results showed that the growth rate of Microcystiswas not significantly influenced by levels of phosphate in the medium but significantly positivelycorrelated with increasing nitrate concentration in the medium. Carrying capacity or themaximum abundance (γ) of Microcystis was influenced by the combination of nitrate andphosphate in the B12 medium. Concentration of phosphate 0.4 ppm in medium combined withnitrate 8−64 ppm could support the highest abundance of Microcystis.

EFFECT OF SAPONIN-PODS EXTRACT ACACIA (ACACIA MANGIUM) TO HEMATOCRIT, HEMOGLOBIN AT TILAPIA (OREOCHROMIS NILOTICUS) Is Yuniar; Win Darmanto; Agoes Soegianto
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Problem of aquaculture shrimp, one of which is wild fish, competitor. Usually to eliminate the use of saponin from tea seed. saponin to kill fish competitors, (tilapia) are entered when filling water. Saponin to kill fish competitors, (tilapia) are entered when filling water. Saponin used from tea seed meal is imported. Indonesia is rich in biological resources of plants / substances that secondary metabolites naturally untapped optimal. The study was conducted in a laboratory scale with a completely randomized factorial design, using a series of six concentrations and three replicates for each species. LC50 analysis using probit analysis. Unfortunately, there was no scientific information for it. It has been done a laboratory work to study chemical compounds of pod Acacia (Acacia mangium) by phytochemical screening and measure those quantitatively. The result of these were that pod Acacia (Acacia mangium) contain alkaloid, flavonoid, tanin, saponin, steroid, and terpenoid. The results of this study indicate LC50-96h values for Pod Acacia mangium at salinity 0, 5, 10 and 15 promil is 5.487 ppm, 4.313 ppm, 3.985 ppm and 2.944. Value hemoglobin at 0promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 11.353±0.63)g/dL,10.607±0.18g/dL, 9.860±0.36 g/dL and 8.80±0.60. Value hemoglobin at 5promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 8.86±1.02 g/dL,7.76±0.943 g/dL, 7.42±0.71 g/dL and 4.68±0.53g/dL. Value hemoglobin at 10 promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 7.34±1.95 g/dL,6.07±0.755 g/dL, 5.23±1.34 g/dL and 3.23±035 g/dL. Value hemoglobin at 15promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 8.570±0.478 g/dL,5.18±0.876 g/dL, 3.42±0386 g/dL and 3.817±0.355g/dL. Value hematocrit at 0promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 35.033±3.32g/dL, 31.2±0.90 g/dL, 29.367±0.97 g/dL and26.60±1.345. Value hematocrit at 5promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 25.633±1.24 g/dL, 23.90±2.68 g/dL, 18.367±2.554 g/dL and 12.867±2.38 g/dL. Value hematocrit at 10 promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 21.567±6.134 g/dL,18.567±1.48 g/dL,14.767±3.46 g/dL and 10.53±3.38 g/dL. Value hematocrit at 15promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 26.100±1.42 g/dL, 15.667±2.79 g/dL,9.933± 2.04 g/dL and 11.933±1.19 g/dL. Saponin of acacia may lower hematocrit and hemoglobin an indication of damage to the red blood cells occurs hemolysis.

Utilization of Fertilizers for Improving The Process Rate of Organic Material Degradation in Anaerobic Reactor Adelia Anju Asmara; Agoes Soegianto; Trisnadi Widyaleksono Catur Putranto; Hairul Amin; Nur Indradewi Oktavitri
IPTEK Journal of Proceedings Series Vol 1, No 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2014i1.225

This study aimed to assess utilization of fertilizers as nutrient in the anaerobic reactor. Nutrient is one important characteristic of waste for the treatment. Nutrient is required for the growth and reproduction process of microorganisms which involved in the anaerobic process. The wastewater used in this research is tofu wastewater. Gandasil B fertilizer has N 6 mg/l and P 20 mg/l. While for Gandasil D fertilizer has N 20 mg/l and P 15mg/l. Anaerobic reactor has 1000 ml of capacity which contain 600 ml of wastewater, 100 ml of liquid fertilizer, and 300 ml of space blank. Anerobic reactor observed for 14 days with 5 observed parameters. Those parameters are TSS, VSS, COD, N, and P. After 14 days, added of Gandasil B fertilizer decreased the concentration of TSS, VSS, COD, N, and P amounted to 57,56%, 26,84%, 80,08%, 96,75%, and 93,18%. compared to the 0th day. While the addition of Gandasil D fertilizer decreased the parameters amounted to 3,48%, 6,21%, 86,72%, 58,10%, and 90,78%. In conclusion, Gandasil B fertilizer in the anaerobic reactor improves the rate of organic material degradation more effectively than Gandasil D fertilizer.Keywords - Anaerobic reactor, COD, fertilizer, N, nutrient, P, TSS, VSS

TOKSISITAS SODIUM LAURYL SULFATE (SLS) TERHADAP BENUR UDANG WINDU (Penaeus monodon Fab.) Agoes Soegianto
Purifikasi Vol 7 No 1 (2006): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25983806.v7.i1.233

Sodium lauryl sulfate (SLS) merupakan bahan dasar pembuatan detergen yang banyak digunakan di masyarakat, karena kemampuannya sebagai surfaktan yang baik. Tingginya kadar SLS di estuaria dapat berbahaya bagi organisme akuatik seperti udang windu prawn (Penaeus monodon, Fab.). Berdasarkan alasan di atas, dilakukan penelitian untuk menentukan toksisitas SLS terhadap benur udang windu. Hasil penelitian menunjukkan bahwa LC50-96 jam SLS terhadap PL2 udang windu adalah 42,43 mg/L, nilai LC50-96 jam SLS terhadap PL15 adalah 51,89 mg/L dan nilai LC50-96 jam SLS terhadap PL25 adalah 336,36 mg/L

Metallothionein analysis and cell damage levels on the liver and gill Of Barbonymus gonionotus In Brantas River, Indonesia Alfiah Hayati; Nita Yuliarini; Agoes Soegianto; Hana Widyana; Inesavira Rindaputri; Nuris Auliya; Putri Ayu Ika
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

This study aimed to analyze the levels of metallothionein and damage to hepatocytes and gills of Barbonymus gonionotus in the Brantas River (upstream and downstream), and the correlation levels of metallothionein in the gills and liver of Barbonymus gonionotus with different sampling time variation (March, June, and September 2016). Fishes were caught using trawl in two stations (Karangkates Reservoir and the Kali Jagir river). Fish gills and livers were taken for histopathological analysis and levels of metallothionein were measured by ELISA method. Gills histopathological analysis showed that the highest damage in Karangkates reservoir dan Kali Jagir river is hyperplasia. Liver histopathological analysis in Karangkates reservoir showed normal hepatocytes meanwhile necrosis is highest damage found in Kali Jagir river. Metallothionein levels of fish in Karangkates reservoir for March, June, and September were 0.18 ng/mL; 0,18 ng/mL; and 0.2 ng/mL (gills fish); 0,18 ng/mL; 0,34 ng/mL; and 0,21 ng/mL (liver fish) respectively. Metallothionein levels of fish in Kali Jagir river for March, June, and September were 0,26 ng/mL; 0,18 ng/mL; and 0,18 ng/mL (gills fish); 0,2 ng/mL; 0,45 ng/mL; 0,19 ng/mL (liver fish) respectively. We conclude that the damage to the gills and liver of Barbonymus gonionotus was mostly found in Kali Jagir River and the highest metallothionein level of Barbonymus gonionotus also presented in Kali Jagir River.

Co-Authors Ach Khumaidi Ach. Khumaidi Achmad Yani Yayan Acivrida Mega Charisma Adam L.H. Suryawan Adelia Anju Asmara Affandi, Moch Alfiah Hayati Ana, Devi Anak Agung Ayu Putri Permatasari Anak Agung Ayu Putri Permatasari Angsukma P. Dewayanti Astra Budi Priatama Bambang Irawan Bambang Yulianto Bambang Yulianto Bambang Yulianto Catur Retnaningdyah Deny Suhermawan Yusup Dianbudiyanto, Wahid Dwi Winarni E Ernawati, E Eko W. Putro Emanuel Maramba Hamu Era Insivitawati Ganden Supriyanto Hairul Amin Hajjarianti, Permata Hana Widyana Holyness Nurdin Singadimedja I Gede Widhiantara I Gede Widhiantara I Made Gde Sudyadnyana Sandhika I Made Gde Sudyadnyana Sandhika I Wayan Rosiana I Wayan Rosiana Indriyasari, Khudrotul Nisa Inesavira Rindaputri Irawati Mei Widiastuti Is Yuniar Jamhari Jamhari Karnaji, Karnaji Leonard Ady Candra M. Yusuf Alamudi Maemonah, Maemonah Marchellina, Ary Matius Victorino Ola Dame Miftakhul Ulum Moch. Syaifullah Moh. Awaludin Adam Moh. Awaludin Adam Muhammad Miftahussurur Mukholladun, Wildanun Ni Kadek Yunita Sari Nia Adiani Primarastri Nining D. Widyayanti Nita Yuliarini Nur Indradewi Oktavitri NUrina Fitriani, NUrina Nuris Auliya Putu Angga Wiradana Putu Angga Wiradana Rahman, T. Rahmatin, Nailul Muthiati Ramli Ramli Rini Fitriasari Pakaya Rizqy, Aimatun Nisfia Saadah, Bidayatus Setiyowati, Putri Ayu Ika Siregar, Muhammad Nuh Fathsyah Suharjono Suharjono Teguh Wirawan Trianingtyas, Deavy Trisnadi Widyaleksono Catur Putranto Widyaleksono, Trisnadi Win Darmanto Yenny Risjani Yesha Ainensis El G. Pelupessy Yuliana Yuliana Yuniar Ade Candra Zagita, Lauditta Chavia

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