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Achmad Dinoto
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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KAJIAN PRA-KLINIS PEMBERIAN ORAL BUBUK KERING-BEKU SUSU KEDELAI YANG DIFERMENTASI OLEH Lactobacillus plantarum AP1 DAN Sphingobacterium SP. TB17 PADA TIKUS Achmad Dinoto; Rita Dwi Rahayu; Sri Purwaningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/305

Abstract

To investigate the effects of oral administration of freeze-dried soymilks fermented by Lactobacillus plantarum AP1 and Sphingobacterium sp. TB17, in preclinical study groups of male Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (CD), basal diet supplemented with 3% (w/w feed) freeze-dried unfermented soymilk (CN), basal diet supplemented with 3% (w/w feed) freeze-dried soymilk fermented by L. plantarum AP1 (CL), and basal diet supplemented with 3% (w/w feed) freeze-dried soymilk fermented by Sphingobacterium sp. TB17 (CK). Our result showed a reduction of serum cholesterol level up to 15% in rats CB after three weeks consuming soymilk fermented by L. plantarum AP1. This yield was relatively higher than that of rats CK consuming soymilk fermented by Sphingobacterium sp. TB17 to reduce serum cholesterol only up to 5%. Although unfermented soymilk (CN) also showed the hypocholesterolomic effect to rats, the reduction of cholesterol serum level was lower than that of rat CL. There were no signifi cant increased in blood glucose and body weight of rats as impact of freeze-dried fermented soymilk. Interestingly, although feed intake tends to increase during fermented soymilk consumption, the body weight of rats did not increase. In general, this study indicated that L. plantarum AP1 soymilk is a promising beneficial food product for the health.
KEANEKARAGAMAN BAKTERI PENGHASIL EKSOPOLISAKARIDA ASAL SALURAN CERNA MANUSIA Achmad Dinoto; Sugiyono Saputra; Agustinus Joko Nugroho; Rita Dwi Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/308

Abstract

Exopolysaccharide (EPS) is a polysaccharide that is excreted by cell as metabolites. Bacterial EPS has been widely used in food and drug industries. In order to find natural products for foods and drugs, the study was conducted to know the diversity of EPS-producing bacteria isolated from human gastrointestinal tracts. As much of 30 bacterial strains were collected from two healthy people and seventeen of collected isolates were investigated for the capability of producing EPS. As the results of identification by partial 16S ribosomal DNA analysis, those seventeen EPS-producing bacterial strains were closely related with already known bacteria (range between 86 to 100% similarity) and could be classified into seven genus, i.e.: Lactobacillus spp. (9 strains), Enterobacter sp. (2 strains), Escherichia sp. (2 strains), Klebsiella sp. (1 strain), Cronobacter sp. (1 strain), Staphylococcus sp. (1 strain), and Alteromonas sp. (1 strain). In addition, member of Lactobacillus group which have closest relationship with species L. plantarum (7 strains) dan L. fermentum (2 strains) were known to dominate the collection of culturable EPS-producing bacteria from human gastrointestinal tracts.
Co-Authors Agustinus Joko Nugroho Rita Dwi Rahayu Sri Purwaningsih Sugiyono Saputra