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All Journal Jurnal Fitofarmaka Indonesia
Rakhmat Ramdhani Alwie
Sekolah Tinggi Teknologi Industri dan Farmasi Bogor
Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology

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AKTIVITAS EKSTRAK ETANOL DAUN SALAM [Syzygium polyanthum (Wight) Walp.] SEBAGAI PENGHAMBAT ENZIM α-GLUKOSIDASE DAN STUDI SECARA IN SILICO Rakhmat Ramdhani Alwie; Esti Mumpuni; Lilik Sulastri; Partomuan Simanjuntak
Jurnal Fitofarmaka Indonesia Vol 8, No 2 (2021): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v8i2.750

Abstract

Diabetes Mellitus (DM) is a degenerative disease that can cause various complications such as cardiovascular, stroke, and hypertension. Antidiabetic drugs that are commonly circulated in the market, have side effects that can harm other organs such as liver, kidneys and other organs. Bay leaves have properties as inhibitors of the α-glucosidase enzyme in vitro and in vivo. The aims of this study was determine the activity of ethanolic extract of bay leaves by ultrasonic extraction method in inhibiting the α-glucosidase enzyme in vitro and in silico. Bay leaves were extracted using 70% and 96% ethanol by ultrasonic method for 15 minutes, and filtered and concentrated using a rotary vacuum evaporator 4 times. The extract obtained was tested for phytochemical content and the activity of α-glucosidase inhibitory assay. The extract with the best IC50 value was then separated using column chromatography (SiO2, dichloromethane, methanol) and produced 7 fractions and tested for α-glucosidase enzyme inhibition. The best fraction (fraction 6) was analyzed by Liquid Chromatography Mas Spectra (LCMS-MS). The results showed that bay leaf extract containing alkaloids, flavonoids, tannins, steroids, phenols, and fraction-6 had the best IC50 of 30.82 ppm. The results of LCMS-MS analysis of chemical compounds as inhibitors of the α-glucosidase enzyme were delphinidin (m/z 303) and had a rerank score of -75.59. In the in silico test using PLANTS docking software.
Aktivitas Penghambatan Enzim α-Glukosidase Senyawa β-Sitosterol dari Fraksi Etil Asetat Daun Salam [Syzygium polyanthum (Wight.) Walp Lilik Sulastri; Muhammad Zulfadhli; Rakhmat Ramdhani Alwie; Sofyan Ramani; Andri Prasetiyo; Vino Soaduon Simanjuntak
Jurnal Fitofarmaka Indonesia Vol 11, No 1 (2024): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v11i1.981

Abstract

Diabetes mellitus, also known as diabetes, is an endocrine disease characterized by sustained high blood sugar levels. Diabetes is caused by the pancreas not producing enough insulin, or the body's cells becoming unresponsive to the effects of the hormone. Common symptoms include thirst, polyuria, weight loss, and blurred vision. If left untreated, the disease can lead to various health complications, such as disorders of the cardiovascular system, eyes, kidneys, and nerves. In general, the treatment of diabetes mellitus is done with insulin therapy and various synthetic drugs, but these treatments have side effects. Another alternative is treatment using herbs, including salam leaves (Syzygium polyanthum (Wight.) Walp) which can be used as an inhibitor for the α-glucosidase enzyme. This study aims to isolate and determine the levels of β-sitosterol from the ethyl acetate fraction of salam leaves which have α-glucosidase enzyme inhibitory activity. Extraction was carried out by maceration using 96% ethanol, then partitioned with ethyl acetate and water (1:1). The ethyl acetate fraction was tested for the inhibitory activity of the α-glucosidase enzyme, then isolated by column chromatography method (SiO2; i.n-hexane-ethylacetate = 10 : 1 ~ 1 : 1, ethylacetate; ii.n-hexane-ethylacetate = 5 : 1 guided by testing enzyme inhibitory activity until pure isolates were obtained. The isolates were identified by spectrophotometry (IR) compared with the standard β-sitosterol compound. The results showed that isolates F.4-5 was a β-sitosterol compound with a concentration of 177.13 mg/g extract and had α-glukosidase enzyme inhibitory activity with an IC50 of 93.7 μg/mL.
Co-Authors Andri Prasetiyo Esti Mumpuni Lilik Sulastri Lilik Sulastri Muhammad Zulfadhli Partomuan Simanjuntak Sofyan Ramani Vino Soaduon Simanjuntak