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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Pharmacon Jurnal Fitofarmaka Indonesia Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Jurnal Sains dan Edukasi Sains Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Farmasi Indonesia Medical Sains : Jurnal Ilmiah Kefarmasian

Esti Mumpuni

Universitas Pancasila

Author-ID : 3521496

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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AKTIVITAS EKSTRAK ETANOL DAUN SALAM [Syzygium polyanthum (Wight) Walp.] SEBAGAI PENGHAMBAT ENZIM α-GLUKOSIDASE DAN STUDI SECARA IN SILICO Rakhmat Ramdhani Alwie; Esti Mumpuni; Lilik Sulastri; Partomuan Simanjuntak
Jurnal Fitofarmaka Indonesia Vol 8, No 2 (2021): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v8i2.750

Diabetes Mellitus (DM) is a degenerative disease that can cause various complications such as cardiovascular, stroke, and hypertension. Antidiabetic drugs that are commonly circulated in the market, have side effects that can harm other organs such as liver, kidneys and other organs. Bay leaves have properties as inhibitors of the α-glucosidase enzyme in vitro and in vivo. The aims of this study was determine the activity of ethanolic extract of bay leaves by ultrasonic extraction method in inhibiting the α-glucosidase enzyme in vitro and in silico. Bay leaves were extracted using 70% and 96% ethanol by ultrasonic method for 15 minutes, and filtered and concentrated using a rotary vacuum evaporator 4 times. The extract obtained was tested for phytochemical content and the activity of α-glucosidase inhibitory assay. The extract with the best IC50 value was then separated using column chromatography (SiO2, dichloromethane, methanol) and produced 7 fractions and tested for α-glucosidase enzyme inhibition. The best fraction (fraction 6) was analyzed by Liquid Chromatography Mas Spectra (LCMS-MS). The results showed that bay leaf extract containing alkaloids, flavonoids, tannins, steroids, phenols, and fraction-6 had the best IC50 of 30.82 ppm. The results of LCMS-MS analysis of chemical compounds as inhibitors of the α-glucosidase enzyme were delphinidin (m/z 303) and had a rerank score of -75.59. In the in silico test using PLANTS docking software.

Konstruksi dan Validasi Protokol Skrining Virtual Berbasis Struktur dengan Kode PDB 3MQE, 3NTG, dan 3LN0 untuk Penemuan Inhibitor Siklooksigenase-2 (COX-2) ESTI MUMPUNI; ARGUN WIDARSA; YANTI SUSILAWATI; OISAN OISAN; ARIEF NURROCHMAD; HARNO DWI PRANOWO; UMAR ANGGARA JENIE; ENADE PERDANA ISTYASTONO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 1 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1025.318 KB)

Cyclooxygenase-2 (COX-2) inhibitors are high demand drugs in the market. However, available COX-2 inhibitors nowadays have many side effects. Therefore, there is still a need to develop more potent selective COX-2 inhibitors and one of the method that has been prove the effectivity and eficiency for new drugs research is in silico. Structure-based virtual screening (SBVS) protocols were developed to find COX-2 inhibitors using the Protein-Ligand ANT System (PLANTS) docking software, SPORES, BKChem and Open Babel. The directory of useful decoys (DUD) dataset for COX-2 was used to validate the protocols retrospectively; the DUD consist of 426 known COX-2 inhibitors and 13289 decoys. Based on criteria value of EF20% and EFmax used in the article from Huang et al (2006) and Yuniarti et al (2011), two validated protocol, AYO_COX2_v.1.1 and AYO_COX2_v.1.2 , showed good results

Produksi Senyawa Kuinina dengan Fermentasi Mikroba Endofit Tanaman Cinchona pubescens Vahl. Esti Mumpuni; Herman Suryadi; Partomuan Simanjuntak
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 2 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1309.288 KB)

This study deals with endophytic microbes, known as a potential source of secondary metabolites. The microbes (F) were isolated from Cinchona pubescens Vahl and fermented in Phoma media, observing the pH, biomass growth and secondary metabolites of alkaloids for 14 days. The result obtained showed that the pH was between 4 and 5, the biomass growth relative stable after 8 days and the production of alkaloids reached its maximum also after 8 days (32.81 mg/l).

Toksisitas dan Anti-inflamasi Senyawa 1,5-Bis(3’-Etoksi-4’-Hidroksifenil)-1,4- Pentadien-3-on (EHP) ESTI MUMPUNI; LESTARI RAHAYU; ARIEF NURROCHMAD
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 1 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.571 KB)

The 1,5-bis(3’-ethoxy-4’-hydroxyphenyl)-1,4-pentadiene -3-one (EHP) is a curcumin analogue. The structure has also been identified before. EHP is obtained, which has been pharmacologically proven to have higher antioxidant activity than curcumin. The objectives of this research were to determine the acute toxicity (LD50) of EHP compound by Weil C.S. method using male mice as experimental animals and to determine its antiinflammatory activity. The LD50 value of the synthesized EHP compound is 6,8675 g/kg body weight (bw) with the criteria of mild toxic, so it is assumed to be safe as drug substance candidate. The anti-inflammatory activity of the compound was determined by obeserved legs swelling index of the mouse. Anti-inflammatory activity of EHP with doses of 137.35 mg, 274.70 mg and 549.40 mg were equal to aspirin with the dose of 90 mg/200 g bw

Sintesis dan Uji Antioksidan Senyawa 1,5-bis(3’-etoksi-4’-hidroksifenil)-1,4-pentadien-3-on (EHP) ESTI MUMPUNI; ENJANG RUSNAWAN; EVI SULASTRI; INDRIANA PERMATASARI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 2 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2012.176 KB)

Synthesis of 1,5-bis(3’-etoxy-4’-hydroxyphenyI)-1,4-pentadiene-3-on (EHP) by aldol condensation method from ethyl Vanilin and acetone, using hydrochloride acid as a catalyst, has been conducted. Identification of the EHP compound was done through interpretation of UV-Vis, FT-IR, GC-MS and NMR data, The antioxidant activity of EHP (IC50 8.66 µg/mL) is higher than curcumin (IC50 11.44 µg/mL) as demonstrated by the DPPH method.

Sifat Fisikokimia dan Aktivitas Antioksidan Crude Fukoidan Hasil Ekstraksi dari Sargassum cinereum Liliek Nurhidayati; Yashinta Fitriani; Syamsudin Abdillah; Esti Mumpuni; Mohamad Rafi
JURNAL ILMU KEFARMASIAN INDONESIA Vol 18 No 1 (2020): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Brown seaweed has been known as a fucoidan source that has various biological activities. Fucoidan could be obtained by various extraction methods. This study aims to extract, characterize and to evaluate the antioxidant activity of crude fucoidan extracted from Sargassum cinereum. Extraction was carried out by refluxing at 100oC for 4 and 5 hours and then precipitated with ethanol. The crude fucoidan was brown powder. The yield with 4 hours extraction time was 2.78% which did not differ significantly from the 5 hours extraction time. Phytochemical screening of crude extract showed that beside fucoidan there were flavonoids, saponins, steroids, triterpenoids, and tannins. The extraction with duration of 4 hours and 5 hours had sulfate content of 16.31% and 11.22%, total carbohydrate content were 22.94% and 22.85%, and in the FTIR spectrum showed OH absorption bands of carbohydrates, fucose, sulfates and uronic acid. Testing of antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhyrazyl) free radical inhibition method produced IC50 values of 721.9 µg/ml and 749.9 µg/ml respectively and it was concluded that crude fucoidan of Sargassum cinereum had weak antioxidant activity.

Analisis In-silico Senyawa Kimia dalam Teh Hijau yang Bekerja pada Aktivator (PPAR-γ) sebagai Antiobesitas Faridah Faridah; Esti Mumpuni; Yudha Iswara Yunanto
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Teh hijau dikenal banyak memiliki manfaat dan umum digunakan masyarakat sebagai antiobesitas, tetapi senyawa aktif yang berpotensi sebagai antiobesitas. Tujuan penelitian untuk mencari bahwa senyawa kimia yang terdapat dalam tanaman teh hijau mempunyai aktivitas sebagai antiobesitas pada reseptor PPAR-γ. Metode penelitian dilakukan dengan cara analisis in silico melalui molecular docking terhadap senyawa yang terdapat dalam tanaman teh hijau untuk mencari senyawa aktif dan memodelkan interaksi senyawa aktif pada reseptor yang berperan sebagai antiobesitas. Software yang digunakan adalah PLANTS, YASARA, ChemSketch, dan Pymol. Mula-mula dilakukan validasi internal pada reseptor PPAR-γ dengan kode 2ATH. Proses docking dilakukan terhadap native ligand, senyawa pembanding dan masing-masing senyawa uji dengan reseptor PPAR-γ yang sama, dan senyawa pembanding yang digunakan sebagai kontrol positif ialah Pioglitazone. Hasil penelitian menunjukan sisi aktif terdapat 3 senyawa aktif dengan sisi aktif ikatan ligan pada reseptor PPAR-γ yaitu ARG288, LYS367, PHE363, HIS323, HIS449, ILE326, MET364, LEU340, CYS285, SER342. Terdapat 3 senyawa aktif yaitu epigalokatekin-3-galat, epikatekin-3-galat dan teaflavin sebagai antiobesitas dengan mekanisme kerja mengaktivasi PPAR-γ.

Penapisan Virtual Senyawa–Senyawa dalam Famili Zingiberaeae sebagai Antiinflamasi Menggunakan Protokol EE_COX2_V.1.0 Esti Mulatsari; Esti Mumpuni; Feriza Sandayu
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Berbagai penelitian tentang sifat-sifat anti-inflamasi dan anti-kanker dari berbagai senyawa dalam tanaman familia Zingiberaceae telah dilakukan baik secara in vivo maupun in vitro. Enzim yang diinduksi dan diekspresikan pada sel-sel inflamasi dan kanker dianggap sebagai target obat yang ideal untuk menghambat peradangan dan tumorgenesis, salah satunya adalah enzim siklooksigenase-2 (COX-2). Dalam penelitian ini telah dilakukan penapisan virtual senyawa dalam tanaman Kaemferia galanga, Curcuma domestica Val., Zingiber officinale dan Curcuma xanthorrhiza. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas senyawa-senyawa tersebut sebagai penghambat enzim COX-2 secara in-silico. Penelitian ini menggunakan EE_COX2_V.1.0, protokol Structure Based Virtual Screening (SBVS) yang telah divalidasi oleh Mumpuni et al. 2014. Protokol EE_COX2_V.1.0 menggunakan berbagai aplikasi terintegrasi seperti SPORES, PLANTS, BkChem, OpenBabel dan PyMOL. Elusidasi moda ikatan dilakukan terhadap senyawa representatif aktif dan tidak aktif untuk melihat interaksi asam amino dalam binding site senyawa. Berdasarkan skor ChemPLP sebagai hasil dari simulasi docking yang dilakukan pada 27 senyawa, ada 3 senyawa yang berpotensi aktif dalam menghambat COX-2, senyawa tersebut antara lain 2-butil-3- (4-metoksifenil) -2- asam propenoat dengan 6 residu asam amino aktif, 6-shogaol dengan 10 residu asam amino aktif dan desmetoksikurkumin dengan 4 residu asam amino yang aktif.

In Silico Analysis of Antiviral Activity of Analog Curcumin Compounds Esti Mulatsari; Titiek Martati; Esti Mumpuni; Nidya Luciana Dewi
Jurnal Jamu Indonesia Vol. 5 No. 3 (2020): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v5i3.173

Some studies state that curcumin analog compounds can improve the bioavailability and biological activity of curcumin. One of the methods to predict the bioactivity of curcumin was computational using molecular docking method. This study has done bioactivity tests of curcumin analog compounds as antiviral using the molecular docking method with the software used are PLANTS, YASARA, MarvinSketch, and Pymol for visualization. This study used analog curcumin compounds derived from previous research. This study used five different viral reseptor types. The maraviroc, docosanol, ribavirin, and zanamivir were used as compound control in this research. The validated target protein consists of 5 (five) receptors with PDB codes 1V2I, 4WEG, 2HWI, 2QAD, and 3ALP. Based on this research, compounds that are predicted active as antiviral on each receptors that are: 2,5-bis(3,5-ditertbutyl-4-hydroxy benzyl)cyclopentanone (1V2I), 1,7- diphenyl-1,6-heptadiene-3,5-dione (4WEG), 1,7-bis(3,4-dibenzyloxiphenyl)-1,6-heptadiene-3,5-dione (2HWI), and 2,5-bis(3,5-ditertbutyl-4-hydroxybenzyl)cyclopentanone (3ALP).

Penapisan Virtual Senyawa Dalam Tanaman Familia Annonaceae Sebagai Ligan Pada Reseptor Estrogen Alfa (ER-α) Esti Mulatsari; Esti Mumpuni; Kevin Sandy
Jurnal Sains dan Edukasi Sains Vol 2 No 2 (2019): Jurnal Sains dan Edukasi Sains
Publisher : Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/juses.v2i2p70-76

Annonaceae adalah familia dengan 800 spesies yang tersebar di seluruh dunia. Beberapa penelitian menunjukkan bahwa annonaceae memiliki aktivitas biologis sebagai antikanker dan telah digunakan sebagai obat herbal secara empiris. Pada penelitian ini dilakukan penapisan virtual beberapa struktur senyawa dari tanaman familia Annonaceae. Tujuan penelitian ini adalah untuk menganalisis dan memperoleh kandidat senyawa yang aktif sebagai ligan pada reseptor estrogen alfa yang dapat memicu timbulnya kanker payudara, selanjutnya akan dilakukan elusidasi moda ikatan senyawa representatif aktif dan inaktif untuk melihat interaksi asam-asam amino pada binding site senyawa tersebut. Penelitian ini menggunakan protokol tervalidasi hasil penelitian Anita dkk., 2012 dengan berbagai aplikasi komputasi seperti SPORES, PLANTS, BKchem, OpenBabel dan PyMol. Hasil penapisan virtual menunjukkan bahwa dari 39 kandidat senyawa ada 18 senyawa dari tanaman familia Annonaceae yang memiliki nilai ChemPLP lebih kecil daripada ligan pembanding, artinya ada 18 senyawa yang memiliki afinitas yang lebih baik terhadap reseptor sehingga dapat mencegah timbulnya kanker payudara.

Co-Authors Agus Kurniawan Andika Muhammad Hidayat ARGUN WIDARSA ARIEF NURROCHMAD ARIEF NURROCHMAD Deni Rahmat Dian Ratih Laksmitawati Enade Perdana Istyastono ENJANG RUSNAWAN EVI SULASTRI FARIDAH FARIDAH Feriza Sandayu Harno Dwi Pranowo Herman Suryadi INDRIANA PERMATASARI Kevin Sandy Lestari Rahayu Liliek Nurhidayati Lilik Sulastri Martati, Titiek Marybet Tri Retno Handayani Mohamad Rafi Mulatsari, Esti Nidya Luciana Dewi Nur Aji OISAN OISAN Partomuan Simanjuntak Partomuan Simanjuntak Rakhmat Ramdhani Alwie Shirly Kumala Siswandono, Siswandono Syamsudin Abdillah Tri Kumala Noerfa Umar Anggara Jenie YANTI SUSILAWATI Yashinta Fitriani Yudha Iswara Yunanto

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