I.G Putu
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Integrity of swamp buffalo sperm on a variety of semen freezing process ., Herdis; Purwantara, B; Supriatna, I; Putu, I.G
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 1 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.795 KB) | DOI: 10.14334/jitv.v4i1.131

Abstract

Sperm of swamp buffalo bulls is easily damaged during freezing process. Acrosomal intact and plasma membrane intact is important factors in fertilization process. This experiment was aimed to study the effect of freezing method on sperm integrity. The result of experiment indicated that the mean of intact acrosomal and the intact plasma membrane for 4 hours of equilibration (52.24 ± 3.70% and 54.34 ± 4.80%) was significant higher (P<0.05) than 2 hours of equilibration (39.00 ± 3.32% and 43.44 ± 4.91%) but was not significantly difference (P>0.05) with 6 hours of equilibration (47.92 ± 4.51% and 51.58 ± 4.25%). There were not significance difference between one step and two step of glycerolization. The best sperm integrity was resulted by freezing method with 4 hours of equilibration and two steps glicerolization.   Key words : Swamp buffalo bulls, sperm integrity, freezing process
Integrity of swamp buffalo sperm on a variety of semen freezing process Herdis .; B Purwantara; I Supriatna; I.G Putu
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.795 KB) | DOI: 10.14334/jitv.v4i1.131

Abstract

Sperm of swamp buffalo bulls is easily damaged during freezing process. Acrosomal intact and plasma membrane intact is important factors in fertilization process. This experiment was aimed to study the effect of freezing method on sperm integrity. The result of experiment indicated that the mean of intact acrosomal and the intact plasma membrane for 4 hours of equilibration (52.24 ± 3.70% and 54.34 ± 4.80%) was significant higher (P<0.05) than 2 hours of equilibration (39.00 ± 3.32% and 43.44 ± 4.91%) but was not significantly difference (P>0.05) with 6 hours of equilibration (47.92 ± 4.51% and 51.58 ± 4.25%). There were not significance difference between one step and two step of glycerolization. The best sperm integrity was resulted by freezing method with 4 hours of equilibration and two steps glicerolization.   Key words : Swamp buffalo bulls, sperm integrity, freezing process