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Herdis .
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Increasing of Reproduction Efficiency of Cattle Through the Application of Synchronization Technology ., Herdis; Surachman, Maman; Kusuma, Ida; Suhana, Epih R
Indonesian Bulletin of Animal and Veterinary Sciences Vol 9, No 1 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.222 KB) | DOI: 10.14334/wartazoa.v9i1.725

Abstract

The constraint of cattle development in Indonesia is low productivity, which was caused by low reproduction efficiency. Unestroes and difficulty of heat detection influenced to the calving interval. This calving interval is one of the causal factors of low reproduction efficiency. The efforts to solve this problem are to control and to predict heat period using synchronization technology. Synchronization technology on catlle has been developed from corpus luteum enucleated, utilizing of gonadotropin, progesteron, estrogen, prostaglandine and modification of those hormones. Application method for synchronization could be done by intra musculair, subcutan implantation, intra vaginal and mixed with food. Each method has its own strength and weaknesses. However, to handle anestroes cases is more effective to use combination of progesteron, PGF2α and estrogen. In order to obtain reproduction efficiency it is suggested to take the anestroes in hand as soon as possible.   Key words : Cattle, synchronization estrous
The Role of Antioxidant for Improving The Quality of Frozen Semen Rizal, Muhammad; ., Herdis
Indonesian Bulletin of Animal and Veterinary Sciences Vol 20, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.838 KB) | DOI: 10.14334/wartazoa.v20i3.937

Abstract

The quality of frozen semen will be reduced if high oxidative metabolism activity in semen occurs, due to the formation of free radical compounds causing lipid peroxidation reaction on sperm plasma membrane. Lipid peroxidation occurs due to the exposure of semen to the oxygen during handling. Lipid peroxidation can be prevented by the addition of antioxidant compounds such as vitamin C, vitamin E, glutathione, and b-carotene, in the semen extender. Results of some researches showed that the addition of various antioxidans in extender can improve the quality of frozen semen of various animals. Key words: Antioxidant, free radical, lipid peroxidation, frozen semen
Improvement of frozen semen quality of Garut Sheep through the addition of α-tocopherol into yolk egg-skim milk diluent ., Herdis; ., Kusuma; Surachman, M; Sutama, I.K; Riza, M; Inounu, I; Purwantara, B; Arifiantini, I
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 1 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.408 KB) | DOI: 10.14334/jitv.v7i1.269

Abstract

The sperm is very fragile to lipid peroxide reaction, that it can easily broken during the process of freezing. To eliminate this consequences an antioxidant agent added into the extender. A research was done to observe the effect of antioxidant agent α-tocoferrol and butylated hydroxytoluene (BHT) presence in the extender on the quality of frozen semen. Once week, semen from six male Garut sheep ages about 2.5 years old was collected using artificial vagina and egg yolk skim-milk diluent used as the extender. The semen were treated in egg yolk skim-milk diluent without antioxidant as control, in egg yolk skim-milk diluent with α- tocoferrol 0,2 g/100 ml diluent and in egg yolk skim-milk diluent with butylated hydroxytoluene 0,2 g/100 ml diluent. The after thawing observation shown that in egg yolk skim-milk diluent with α- tocoferrol had life percentage (75.0 ± 3.5% vs 64.8 ± 7.8%) and membrane intact percentage (65.8 ± 6.8 % vs 55.2 ± 8.3%) significantly higher than control (P<0,05) but insignificantly different from with BHT addition. The presence of α-tocoferrol in the diluent, the motility percentage consideraly higher (P<0.05) than (45.8 ± 3.8%) using BHT addition (40.0 ± 4.5%) but not different from control (41.7 ± 4.1%); while acrosomal intake percentage after α-tocoferrol (54.8% ± 3.3%) expressively higher (p,0.05) than BHT addition (49.7 ± 3.6%) or control (49.8 ± 3.5%). In conclusion the presence of α-tocoferrol in the diluent could improve the quality of Garut sheep frozen semen.   Key words: Antioxidant, sperm, Garut sheep
The quality of Garut ram liquid semen in Tris egg yolk extender to the sucrose supplementation ., Yulnawati; ., Herdis
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.049 KB) | DOI: 10.14334/jitv.v14i1.362

Abstract

The successful program of artificial insemination in sheep is determined by the quality of sperm that are used. Therefore, it is important to maintain the quality of sperm during and after storage in low temperature. The research was conducted to study the influence of sucrose in the Tris-yolk extender in maintaining the quality of Garut ram sperm during preservation in reaction tube for four days at 5°C. Ejaculated sperm was collected once a week for five weeks using artificial vagina from same ram. Semen was divided into four groups of extenders, i.e. Tris egg yolk 20% (TKT), TKT + 0,1% sucrose, TKT + 0,3% sucrose and TKT + 0,5% w/v sucrose. The results showed that the percentage of motility on day four (D-4) of storage in TKT (41.00 ± 2.0%) was lower (P<0.05) than that in TKT + 0.3% (48.00 ± 2.45%) and TKT + 0.5% (51.00 ± 3.74%), however there was no significant different (P>0.05) from TKT + 0.1% (45.00 ± 3.16%). Whereas the percentage of live cells (%H) on D-4 in TKT, TKT + 0.1%, TKT + 0.3% and TKT + 0.5% were 55.00 ± 2.19%; 57.20 ± 2.79%; 59.20 ± 3.25% and 61.20 ± 3.60%, respectively. Meanwhile, the percentage of membrane integrity (MPU) in TKT (51.80 ± 1.94%) was significantly different (P<0.05) from TKT + 0.3% (57.40 ± 2.65%) and TKT + 0.5% (59.20 ± 3.66%), however there was no significant different (P>0.05) from TKT + 0.1% (54.80 ± 2.86%). In conclusion, the addition of sucrose 0.3% w/v into TKT extender could maintain the quality of Garut ram sperm more efficiently and better than TKT extender. Key Words: Sucrose, Tris Egg Yolk, Preservation, Garut Sheep
Role of various sugars in improving frozen semen quality of Garut ram Rizal, Muhammad; ., Herdis; Boediono, Arief; Aku, Achmad Selamet; ., Yulnawati
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.223 KB) | DOI: 10.14334/jitv.v11i2.516

Abstract

Ram spermatozoa are sensitive to extreme changes in temperature during the freeze-thawed process. The present study was conducted to examine the effects of addition of various sugars in Tris extender on sperm cryosurvival of Garut ram. Semen was collected using an artificial vagina from three mature rams once a week. Immediately after initial evaluation, semen was divided into five parts and diluted with Tris extender (control), Tris extender + 0.4% dextrose, Tris extender + 0.4% raffinose, Tris extender + 0.4% trehalose, and Tris extender + 0.4% sucrose, respectively. Semen was loaded in to 0.25 ml mini straw with the concentration of 200 million or 800 million motile spermatozoa per ml. Semen was equilibrated at 5oC for three hours, then frozen and stored in liquid nitrogen for seven days. Quality of processed-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), intact acrosome cap (IAC), and intact plasma membrane (IPM) were evaluated after dilution, equilibration, and thawing, respectively. Data were analyzed using completely randomized design with five treatments and six replicates. Means were compared significant difference test at 0.05 significant level. Results of this research showed that there was no significantly difference (P>0.05) between treatments for all sperm quality parameters after dilution and equilibration. Mean percentages of post thawing MS, LS, IAC, and IPM for dextrose (54.00; 68.00; 66.60, and 57.83%), raffinose (50.00; 64.33; 61.80, and 61.75%), trehalose (50.83; 65.67; 61.40 and 57.75%), and sucrose (49.00; 66.80; 58.50 and 58.50%) were significantly (P<0.05) higher than control (40.83; 52.67; 54.60, and 49.40%) respectively. In conclusion, addition of 0.4% dextrose, raffinose, trehalose or sucrose in Tris extender are effective in improving frozen semen quality of Garut ram. Key Words: Sugars, Tris extender, Frozen Semen Quality, Garut Ram
Role of seminal plasma in maintaining quality of ram epididymal spermatozoa preserved at low temperature (3–5oC) ., Herdis; Aku, Achmad Selamet; Rizal, Muhammad; Surachman, Maman
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.72 KB) | DOI: 10.14334/jitv.v11i4.539

Abstract

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, because the spermatozoa is motile and has ability of fertilizing the oocyte. The purpose of this research was to examine the effect of addition of seminal plasma on the quality of ram cauda epididymal spermatozoa preserved at 3–5oC. Collected-spermatozoa was divided into five parts then centrifuged at 3,000 rpm for 30 min and the supernatant was removed. One-fifth was diluted with Tris extender (Tris) and the remaining was added 0.5 ml ram seminal plasma then diluted with Tris extender (PS-Tris), 15% AndroMed + 85% distilled water (PS-AM15), 20% AndroMed + 80% distilled water (PS-AM20), and 25% AndroMed + 75% distilled water (PS-AM25), respectively. Quality of collected-spermatozoa including spermatozoa concentration (SC), percentages of motile spermatozoa (MS), live spermatozoa (LS), abnormal spermatozoa (AS), cytoplasmic droplet (CD), and intact plasma membrane (IPM) were evaluated. Percentages of MS, LS, and IPM of diluted-spermatozoa was evaluated every day during preserved at 3–5oC for three days. Results of this study showed that mean SC, MS, LS, AS, CD, and IPM of fresh spermatozoa were 11,660 million/ml, 65, 81, 7.6, 10.2, and 82.2%, respectively. Addition of seminal plasma in cauda epididymal spermatozoa prior to dilution could maintain the quality of spermatozoa during preserved at 3–5oC for three days. At day-4 of storage, percentages of MS, LS, and IPM for PS-Tris (43, 58, and 59.2%), PS-AM20 (40.5, 53.75, and 53.75%), and PS-AM25 (40, 54.8, and 55.2%) were significantly (P<0.05) higher than Tris (21, 34.8, and 33.6%) and PS-AM15 (20, 40, and 42.2%). In conclusion, addition of seminal plasma in ram cauda epididymal spermatozoa prior to dilution with Tris or 20 and 25% AndroMed extenders could maintain the quality of spermatozoa during preserved at 3–5oC for three days, and could be used for artificial insemination or in vitro embryo production programs. Key Words: Cauda Epididymal Spermatozoa, Seminal Plasma, Tris, AndroMed, Ram
Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3–5oC Rizal, Muhammad; ., Herdis; Surachman, Maman; Mesang-Nalley, W. Marlene
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.075 KB) | DOI: 10.14334/jitv.v13i1.591

Abstract

In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE) buck spermatozoa preserved at 3–5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tubes then centrifuged at 3,000 RPM for 30 min. Supernatant of the first tube was mixed again with Pasteur pipette (treatment A or control). Supernatant of the second tube was removed (treatment B or without seminal plasma). Supernatant of the third tube was removed and changed with Priangan ram seminal plasma in the same volume (treatment C). Semen was diluted with Tris extender containing 20% egg yolk and stored in refrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) was evaluated every day during storage at 3–5oC for three days. Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, spermatozoa abnormal, and IPM of PE buck fresh semen, respectively was 0.68 ml, cream, thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12% and 84%. At day-4 of storage, percentages of MS, LS, and IPM for treatment C (40, 52.2 and 51.6%) was significantly (P<0.05) higher than that of: treatment B (31, 44.8 and 45.2%) and treatment A (11, 15.6 and 14.8%). In conclusion, seminal plasma of Priangan ram could maintain the quality of PE buck semen preserved at 3–5oC for three days, and it prevent semen from coagulation. Key Words: Seminal Plasma, Priangan Ram, Spermatozoa Viability, PE Buck
The quality of spotted buffalo epididymal sperm with addition of raffinose as external cryoprotectant ., Yulnawati; ., Herdis; Maheshwari, H; Rizal, M
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.347 KB) | DOI: 10.14334/jitv.v13i1.592

Abstract

The aims of this research was to obtain the quality of Spotted buffalo epididymal sperm in different kind of extender in the three stages of cryopreservation (after dilution, post equilibration and post thawing). Spermatozoa was collected with combination of slicing and pressure method into the epididymal tissue in Andromedâextender. Soon after diluted and equilibrated, epididymal spermatozoa was cryopreserved in liquid nitrogen (-196°C).  The result showed that the percentage of motility after thawing in Andromedâ + raffinose 0.4% (47.0 ± 2.4%), was significantly different (P<0.05) from that of control (41.0 ± 2.0%), but there was no significantly different (P>0.05) from that of Andromedâ + raffinose 0.2% (46.0 ± 2.0%). The percentage of live sperm after thawing in control (52.2 ± 2.5%), was the lowest and significantly different (P<0.05) from that of Andromedâ + raffinose 0.2% (59.2 ± 2.6%) and Andromedâ + raffinose 0.4% (58.8 ± 3.1%). Moreover, the percentage of membrane integrity after thawing in control, Andromedâ + raffinose 0.2% and Andromedâ + raffinose 0.4% was 68.0 ± 1.1%; 67.2 ± 1.6% and 67.6 ± 1.2%, respectively. There was no significantly different (P>0.05) in the percentage of membrane integrity from all treatments. In conclusion, the addition of 0.2 and 0.4% raffinose into Andromedâ extender could improve the percentage of motility and viability of post thawing spotted buffalo epididymal spermatozoa. Key Words: Epididymal Sperm, Cryopreservation, Raffinose, Spotted Buffalo
Integrity of swamp buffalo sperm on a variety of semen freezing process ., Herdis; Purwantara, B; Supriatna, I; Putu, I.G
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 1 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.795 KB) | DOI: 10.14334/jitv.v4i1.131

Abstract

Sperm of swamp buffalo bulls is easily damaged during freezing process. Acrosomal intact and plasma membrane intact is important factors in fertilization process. This experiment was aimed to study the effect of freezing method on sperm integrity. The result of experiment indicated that the mean of intact acrosomal and the intact plasma membrane for 4 hours of equilibration (52.24 ± 3.70% and 54.34 ± 4.80%) was significant higher (P<0.05) than 2 hours of equilibration (39.00 ± 3.32% and 43.44 ± 4.91%) but was not significantly difference (P>0.05) with 6 hours of equilibration (47.92 ± 4.51% and 51.58 ± 4.25%). There were not significance difference between one step and two step of glycerolization. The best sperm integrity was resulted by freezing method with 4 hours of equilibration and two steps glicerolization.   Key words : Swamp buffalo bulls, sperm integrity, freezing process
The quality of spotted buffalo epididymal sperm with addition of raffinose as external cryoprotectant Yulnawati .; Herdis .; H Maheshwari; M Rizal
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.347 KB) | DOI: 10.14334/jitv.v13i1.592

Abstract

The aims of this research was to obtain the quality of Spotted buffalo epididymal sperm in different kind of extender in the three stages of cryopreservation (after dilution, post equilibration and post thawing). Spermatozoa was collected with combination of slicing and pressure method into the epididymal tissue in Andromedâextender. Soon after diluted and equilibrated, epididymal spermatozoa was cryopreserved in liquid nitrogen (-196°C).  The result showed that the percentage of motility after thawing in Andromedâ + raffinose 0.4% (47.0 ± 2.4%), was significantly different (P<0.05) from that of control (41.0 ± 2.0%), but there was no significantly different (P>0.05) from that of Andromedâ + raffinose 0.2% (46.0 ± 2.0%). The percentage of live sperm after thawing in control (52.2 ± 2.5%), was the lowest and significantly different (P<0.05) from that of Andromedâ + raffinose 0.2% (59.2 ± 2.6%) and Andromedâ + raffinose 0.4% (58.8 ± 3.1%). Moreover, the percentage of membrane integrity after thawing in control, Andromedâ + raffinose 0.2% and Andromedâ + raffinose 0.4% was 68.0 ± 1.1%; 67.2 ± 1.6% and 67.6 ± 1.2%, respectively. There was no significantly different (P>0.05) in the percentage of membrane integrity from all treatments. In conclusion, the addition of 0.2 and 0.4% raffinose into Andromedâ extender could improve the percentage of motility and viability of post thawing spotted buffalo epididymal spermatozoa. Key Words: Epididymal Sperm, Cryopreservation, Raffinose, Spotted Buffalo
Co-Authors Achmad Selamet Aku Amrozi Anak Agung Gede Sugianthara Arief Boediono B Purwantara B Purwantara Bambang Purwantara Epih R Suhana H Maheshwari I Arifiantini I Inounu I Supriatna I.G Putu I.K Sutama Ida Kusuma Insun Sangadji Kusuma . M Riza M Rizal M Surachman Maman Surachman Maman Surachman Muhammad Riyadhi Muhammad Rizal MUHAMMAD RIZAL Nasrullah . Santoso . Wilmientje Marlene Mesang Nalley Yulnawati .