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Molecular characteristic and pathogenicity of Indonesian H5N1 clade 2.3.2 viruses NLPI, Dharmayanti; R, Hartawan; DA, Hewajuli; ., Hardiman; H, Wibawa; ., Pudjiatmoko
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 2 (2013)
Publisher : Indonesian Animal Sciences Society

The outbreak of disease in late 2012 in Indonesia caused high duck mortality. The agent of the disease was identified as H5N1 clade 2.3.2. The disease caused economic loss to the Indonesian duck farmer. The clade 2.3.2 of H5N1 virus has not previously been identified, so this study was conducted to characterize 4 of H5N1 clade 2.3.2 viruses by DNA sequencing in eight genes segment virus namely HA, NA, NS, M, PB1, PB2, PA and NP.Â The pathogenicity test of clade 2.3.2 viruses in ducks was compared to clade 2.1.3 viruses which predominat circulating in Indonesia. Results of phylogenetic tree analysis showed that the four of clade 2.3.2 viruses isolated in 2012 was the new introduced virus from abroad. Further analysis showed eight genes were in one group with the clade 2.3.2 viruses, especially those from VietNam and did not belong to Indonesia viruses group. The pathogenicity test in ducks showed that virus H5N1 clade 2.3.2 and clade 2.1.3 have similar clinical symptoms and pathogenicity and cause death in 75% of ducks on days 3-6 after infection. Key Words: H5N1 Virus, Clade 2.3.2, Clade 2.1.3, Phylogenetic Tree, Pathogenecity

Screening test for detection of Newcastle Disease, Avian Influenza and Infectious Bronchitis viruses using multiplex reverse transcriptase polymerase chain reaction approach R, Hartawan; NLPI, DharmayantI
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

Numerous viral pathogens are circulated in the environment of commercial chicken farms that causing the difficulty in the confirmation of diagnosis. A breakthrough on the diagnosis technique is required in order to deal with multiple viral infections. Ideally, the approach should have not only high accuracy but also economical and straightforward. The objective of this research is to develop a rapid multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) diagnostic method for three common infectious viral diseases in poultry, Newcastle Disease (ND), Avian Influenza (AI) subtype H5N1 and Infectious Bronchitis (IB). This study was successful in developing and optimizing the mRT-PCR for these three pathogens in a single reaction test. Testing 67 field samples from Sukabumi district revealed the presence of several targeted viruses. Key Words: Screening Test, Multiplex RT-PCR, Newcastle Disease, Avian Influenza, Infectious Bronchitis

Screening test for detection of Newcastle Disease, Avian Influenza and Infectious Bronchitis viruses using multiplex reverse transcriptase polymerase chain reaction approach Hartawan R; DharmayantI NLPI
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 3 (2013): SEPTEMBER 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Numerous viral pathogens are circulated in the environment of commercial chicken farms that causing the difficulty in the confirmation of diagnosis. A breakthrough on the diagnosis technique is required in order to deal with multiple viral infections. Ideally, the approach should have not only high accuracy but also economical and straightforward. The objective of this research is to develop a rapid multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) diagnostic method for three common infectious viral diseases in poultry, Newcastle Disease (ND), Avian Influenza (AI) subtype H5N1 and Infectious Bronchitis (IB). This study was successful in developing and optimizing the mRT-PCR for these three pathogens in a single reaction test. Testing 67 field samples from Sukabumi district revealed the presence of several targeted viruses. Key Words: Screening Test, Multiplex RT-PCR, Newcastle Disease, Avian Influenza, Infectious Bronchitis

Molecular characteristic and pathogenicity of Indonesian H5N1 clade 2.3.2 viruses Dharmayanti NLPI; Hartawan R; Hewajuli DA; Hardiman .; Wibawa H; Pudjiatmoko .
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 2 (2013): JUNE 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The outbreak of disease in late 2012 in Indonesia caused high duck mortality. The agent of the disease was identified as H5N1 clade 2.3.2. The disease caused economic loss to the Indonesian duck farmer. The clade 2.3.2 of H5N1 virus has not previously been identified, so this study was conducted to characterize 4 of H5N1 clade 2.3.2 viruses by DNA sequencing in eight genes segment virus namely HA, NA, NS, M, PB1, PB2, PA and NP. The pathogenicity test of clade 2.3.2 viruses in ducks was compared to clade 2.1.3 viruses which predominat circulating in Indonesia. Results of phylogenetic tree analysis showed that the four of clade 2.3.2 viruses isolated in 2012 was the new introduced virus from abroad. Further analysis showed eight genes were in one group with the clade 2.3.2 viruses, especially those from VietNam and did not belong to Indonesia viruses group. The pathogenicity test in ducks showed that virus H5N1 clade 2.3.2 and clade 2.1.3 have similar clinical symptoms and pathogenicity and cause death in 75% of ducks on days 3-6 after infection. Key Words: H5N1 Virus, Clade 2.3.2, Clade 2.1.3, Phylogenetic Tree, Pathogenecity

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