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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Kesehatan Gigi (Dental Health Journal) Journal of Health and Nutrition Research
Pande Putu Purwaningsih
Poltekkes Kemenkes Denpasar Jurusan Keperawatan Gigi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Elusidasi Awal Daya Hambat Ekstrak Etanol Daun Ketapang (Terminalia Catappa L.) Terhadap Pertumbuhan Staphylococcus Aureus ATCC25923 Penyebab Gingivitis Putu Pande Purwaningsih; Ida Bagus Darmayasa; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p08

Abstract

The leaf of “Ketapang” (Terminalia catappa L.) can be used as traditional medicine. The use of natural material is generally considered to be safer and has less side effect as compared to the use of modern medicine. This study aims at analysing the early elucidation of resistor property of the ethanol extract of the “ketapang” leaf against the growth of Staphylococcus aureus ATCC25923 as the cause of gingivitis, and to identify the active compound contained in the “ketapang” leaf. This study uses Complete Random Design (RAL) with 7 treatments of the extract concentration of “ketapang” leaf namely concentration of 1%, 2%,3%, 4%, 5%, positive control (chlorexidine) and negative control (ethanol). The discovery of the resistor efficacy of “ketapang” leaf extract against S. aureus bacteria using Kirby Beuer method has been indicated by the development of clear zone as the result of the application of anti-bacteria compound which is diffused in bacteria growth media. The finding of the study shows that the higher the concentration of the extract (5%), the wider the development of the zone of the resistor area. The effectiveness value of the anti-bacteria of “ketapang” leaf extract is not better than the antiseptic being tested. The result of profile test of phytochemical test shows that the extract of “ketapang” leaf contains tannin, saponin, terpenoid and flavonoid. The result of GC-MS test, it has been identified five compounds being contained in fraction IV namely Alpha Terpinolene, Cyclohexanol 5-methyl, Hexadecanoic acid, Octadecanoic acid, 1.2Benzenedicarboxylic acid. five active compounds that we found have the potentials of functioning as anti-bacteria.
ANALISIS FAKTOR RESIKO YANG MEMPENGARUHI KARIES GIGI PADA ANAK SD KELAS V-VI DI KELURAHAN PEGUYANGAN KANGIN TAHUN 2015 Pande Putu Purwaningsih; Ni Made Sirat
Jurnal Kesehatan Gigi (Dental Health Journal) Vol 4, No 1 (2016)
Publisher : Jurusan Keperawatan Gigi Poltekkes kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jkg.v4i1.502

Abstract

Dental Caries is one of the dental cavity disease as indicated by the damage of the email and dentin layers of the teeth which occurs progressively. The result of the 2013 Riskesdas with comprehensive data collection on dental health including dental health status indicator, service coverage indicators and dental health behaviors where one of the of the age groups being examined are those ≥ 12 years. The objective of the study is to analyze the factors which are related to the caries in elementary school students. The study used cross sectionaldesign being conducted for students of elementary schools as V-VI in the District of Peguyangan Kangin with the sample size of 85 persons. The data analysis used bivariateand multivariate with logistical regression. TheChi-Squareshows that there is a significant relation between oral hygiene and caries with p score = 0,001, OR = 7,714 ,CI 95% =2,064 – 28,828. The teeth brushing behavior influence significantly towards caries with OR score = 20, CI 95% = 5,951 – 69,636 dan p = 0,000 . The behavior in brushing teeth is independentlyand is significant towards caries with score of adjust odd ratio = 18, CI 95% = 4,611 – 74,582 dan p = 0,000 , The dining pattern influences significantly towards caries with OR score = 6, CI 95% = 1,304 – 28,438 and p = 0,012. The parents’ education does not influence significantly towards caries where OR score = 1, CI 95% = 0,486 – 3,854 and p = 0,552and drinking water does not influence significantly towards caries with OR score = 0,753 CI 95% = 0,281 -2,023andp = 0,573. Therefore, it can be concluded that the behavior in brushing teeth, oral hygiene and dining pattern significantly influence dental caries.It is suggested that health care institutions to increase education through UKGS as part of the effort in improving dental health status and oral hygiene of elementary school students.
Nutritional Profile and Potent Antioxidant Activity of Saraba Keke: A Traditional Manadonese Functional Beverage Kusmiyati, Kusmiyati; Tirtawati, Gusti Ayu; Terok, Maria; Purwaningsih, Pande Putu
Journal of Health and Nutrition Research Vol. 5 No. 1 (2026)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jhnresearch.v5i1.1062

Abstract

Saraba Keke is a traditional Manadonese beverage, but its nutritional and functional properties are not well-documented. This study aimed to characterize the physicochemical properties and antioxidant potential of a specific powdered variant of Saraba Keke Manado, produced by a local producer who adheres to traditional formulation methods to ensure authenticity. The study employed a descriptive analytical design. Physicochemical analysis included proximate analysis (moisture, ash, fat, protein, and carbohydrates by difference) and qualitative and quantitative phytochemical screening. Proximate analysis revealed a composition of moisture (2.92%), ash (1.58%), fat (5.42%), protein (7.63%), and a high carbohydrate content (82.45%), likely stemming from the complex spice base and traditional sweeteners used in the recipe. Qualitative screening showed that alkaloids were strongly present (+++), while flavonoids, tannins, saponins, triterpenoids, and phenolics were detected at a lower level (+). Quantitative analysis showed mean concentrations of tannins (2.63 mg/g), flavonoids (12.92 mg/g), and total phenolics (6.23 mg/g). The DPPH assay demonstrated very strong antioxidant activity with an IC50 value of 35.01 ppm, which is explicitly classified as very strong because it is below the 50 ppm threshold. While these findings reflect the profile of the specific sample analyzed, they provide a scientific baseline for future standardization. Saraba Keke Manado possesses a notable nutritional profile rich in bioactive phytochemicals, particularly flavonoids and phenolics, supporting its potential as a functional health beverage.
Co-Authors Gusti Ayu Tirtawati, Gusti Ayu Ida Bagus Gede Darmayasa Kusmiyati Kusmiyati Maria Terok Ni Made Sirat Ni Putu Adriani Astiti