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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS ISM (Intisari Sains Medis) : Jurnal Kedokteran Advances in Tropical Biodiversity and Environmental Sciences
Ni Putu Adriani Astiti
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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GC-MS Analysis of Active and Applicable Compounds in Methanol Extract of Sweet Star Fruit (Averrhoa carambola L.) Leaves Ni Putu Adriani Astiti; Yan Ramona
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.12

Abstract

The leaves of star fruit plants (Averrhoa carambola) have been traditionally used to cure many diseases, such as hypertension and fever. Besides, the leaves of this plant have also widely been used as the main raw material in lawar (a Balinese traditional food) making. In this research, the methanol extract of this plant leaves was analyzed and identified for active compound contents. The main objective of this research was to investigate types of possible active and applicable compounds contained in such leaves, previously extracted/macerated in methanol solution. Analysis was conducted by applying GC-MS instrumentation using methanol solution. The results showed that 10 possible active and applicable compounds (Butane, 1,1-diethoxy-3 methyl-(CAS)1.1-DII, Dodecanoic acid, methyk ester(CAS) methyl, Dodecanoic acid, methyl ester (CAS) Ethyl Laun, Pentadecanoic acid ethyl ester, Hexadecanoic acid methyl ester (CAS) Methyl pa, OCTADECA 9.12 DIENOIC ACID METHYL, 9-Octadecenoic acid methyl ester (E)-(CAS), Octadecanoic acid methyl ester, and (E) 9-Octadecanoic acid ethyl ester) were identified in methanol extract of such leaves. Among those compounds, Butane, 1,1-diethoxy-3-methyl and Hexadecanoic acid, methyl ester was two most abundance constituents with percentage of peak areas of 35.67% and 26.93%, respectively.
IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (Vitis vinifera L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION Ni Nyoman Nila Arieswari; Ida Ayu Astarini; Ni Putu Adriani Astiti; Jeremy Pramana
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.116 KB) | DOI: 10.24843/IJBB.2018.v06.i01.p04

Abstract

One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.
PERTUMBUHAN ANGGREK Vanda helvola PADA MEDIA YANG DIPERKAYA JUS TOMAT Yully Muharyati; Made Ria Defiani; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2015.v02.i02.p03

Abstract

Vanda is one of the most cultivated orchid species.  In nature, Vanda is endangered because it was exploited in large quantities. Orchid genetic viability can be maintained by embryo culture. The study aimed to investigate the growth of Vanda helvola seeds during 12 weeks after cultured. Media Murashige and Skoog (MS) added with tomato juice 100 g/L was used as culture medium. The results showed that embryo could grow on MS medium on stadium 6th. Protocorm started to form at 9 weeks after cultured. At 12 weeks, percentage of protocorm formed reach almost 24.1%. Tomato juice improved the growth of protocorm of  V.helvola. Key words: Medium, protocorm, Vanda helvola
Identifikasi Senyawa Aktif Ekstrak Daun Tembelekan (Lantana camaraL.) Yang Berpotensi Sebagai Pengendali JamurFusarium solani (Mart.) Sacc. Penyebab Layu Batang dan Busuk Akar Tanaman Pisang Kepok(Musa paradisiacaforma typica) Ni Made Dewi Wahyuni; Ni Putu Adriani Astiti; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p08

Abstract

Banana plants have been used as parts of ritualism by Hindu’S people in Bali nevertheles these plants are often infected by fungal pathogens. Fusarium sp is one of the most common fungal pathogen infecting these plants. Commonly to release this pathogen the usage synthetic fungicides. To reduce the use of these synthetic fungicides, alternative method such as using natural compound of fungicides has been researched. The main objective of this research was to investigate the compounds of tembelekan leaf extract to control the growth of F. solani (Mart.) Sacc, the causative agent of stem wilt and root rot in kapok banana by using GC-MS. The result of identification using GC-MS identified that there are four compounds is 2,2,4-Trimethyl-1,3-pentanediol diisobutyrate, Hexadecanoic acid, Terephthali acid, and Squalene.
Elusidasi Awal Daya Hambat Ekstrak Etanol Daun Ketapang (Terminalia Catappa L.) Terhadap Pertumbuhan Staphylococcus Aureus ATCC25923 Penyebab Gingivitis Putu Pande Purwaningsih; Ida Bagus Darmayasa; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p08

Abstract

The leaf of “Ketapang” (Terminalia catappa L.) can be used as traditional medicine. The use of natural material is generally considered to be safer and has less side effect as compared to the use of modern medicine. This study aims at analysing the early elucidation of resistor property of the ethanol extract of the “ketapang” leaf against the growth of Staphylococcus aureus ATCC25923 as the cause of gingivitis, and to identify the active compound contained in the “ketapang” leaf. This study uses Complete Random Design (RAL) with 7 treatments of the extract concentration of “ketapang” leaf namely concentration of 1%, 2%,3%, 4%, 5%, positive control (chlorexidine) and negative control (ethanol). The discovery of the resistor efficacy of “ketapang” leaf extract against S. aureus bacteria using Kirby Beuer method has been indicated by the development of clear zone as the result of the application of anti-bacteria compound which is diffused in bacteria growth media. The finding of the study shows that the higher the concentration of the extract (5%), the wider the development of the zone of the resistor area. The effectiveness value of the anti-bacteria of “ketapang” leaf extract is not better than the antiseptic being tested. The result of profile test of phytochemical test shows that the extract of “ketapang” leaf contains tannin, saponin, terpenoid and flavonoid. The result of GC-MS test, it has been identified five compounds being contained in fraction IV namely Alpha Terpinolene, Cyclohexanol 5-methyl, Hexadecanoic acid, Octadecanoic acid, 1.2Benzenedicarboxylic acid. five active compounds that we found have the potentials of functioning as anti-bacteria.
Potensi Ekstrak Ethanol Daun Salam Dan Air Rebusan Daun Salam Untuk Memperbaiki Profil Lipid (Ldl-Kolesterol) Darah Pada Tikus Wistar Yulidia Iriani; Yan Ramona; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p09

Abstract

Hyperlipidimia merupakan suatu kelainan yang memicu terjadinya penyakit kardiovascular yang jumlah penderitanya cenderung mengalami peningkatan. Kelainan ini disebabkan oleh pola makan masyarakat yang cenderung mengkonsumsi makanan tinggi lemak, tetapi rendah kadar serat. Pemakaian obat paten, seperti simvastatin merupakan pilihan utama saat ini untuk mengatasi masalah tersebut. Namun, pemakaian obat ini dalam waktu lama tidak dianjurkan karena dapat menyebabkan efek samping merugikan bagi tubuh, sehingga perlu dicari bahan alam yang mempunyai efek sama dengan obat tersebut. Pada penelitian ini, potensi hypolipidemia daun salam diuji pada tikus jantan galur Wistar karena secara empirik daun tanaman ini sering dipakai sebagai obat tradisional. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak etanol daun salam (EEDS) dan rebusan daun salam (ARDS) terhadap menurunkan LDL-Kolesterol tikus galur Wistar. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan delapan kelompok perlakuan yaitu perlakuan dengan aquades (kontrol negatif), simvastatin 0,36 mg/kgBB (kontrol positif), EEDS 252mg/kgBB (EEDS 252), 504mg/kgBB (EEDS 504), 756mg/kgBB (EEDS 756), ARDS 25%/kgBB (ARDS 25), 50% mg/kgBB (ARDS 50), dan 75% mg/kgBB (ARDS 75). Perlakuan diberikan selama satu minggu pada tikus yang sebelumnya diberi pakan kaya lemak selama satu bulan. Hasil penelitian menunjukkan terjadinya penurunan kadar LDL-Kolesterol secara signifikan (p?0.05) pada semua kelompok perlakuan dengan kisaran potensi sebesar 8,00±0,82% sampai 56,16 ± 5,69% (< kontrol negatif dengan penurunan sebesar 6,25 ± 0,50%). Bahkan pada perlakuan 756mg/kgBB EEDS (EEDS 756) menunjukkan hasil yang lebih baik (p?0.05) daripada kontrol positif (simvastatin). Efek hypolipidemia dari ekstrak daun salam ini diduga disebabkan oleh golongan senyawa-senyawa flavonoid, alkaloid, dan tannin yang terkandung dalam ekstrak daun salam. Kata kunci: Daun salam, LDL-Kolesterol, Tikus Wistar
Aktivitas Antioksidan Ekstrak Kulit Jeruk Keprok (Citrus reticulata Blanco) So’e Sebagai Teh Tradisional Deglory Tunmuni; Ni Putu Adriani Astiti; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p11

Abstract

The Citrus So'e plant is one of the leading community plants of the people of East Nusa Tenggara (NTT). The So'e tangerine is used as a raw material in the manufacture of orange juice and the peel has not been used. This research was conducted to utilize So'e tangerine peel as a traditional tea. The parameters observed included the active compound group, antioxidant activity, the type of active compound for So'e tangerine peel and the public's perception of the traditional So'e tangerine peel tea. The research method was carried out by qualitative phytochemical tests, test for the content of flavonoids, phenolics, and tannins by spectaphotometry, antioxidant tests using the DPPH method, testing for the types of active compounds using GC-MS and organoleptic tests to determine the level of consumer preference. The results showed that So'e tangerine peel extract had active compounds, namely flavonoids, phenolics, tannins, steroids, triterpenoids and alkaloids and So'e tangerine peel extract contained flavonoids = 253.901 mg / 100g QE, phenolic = 61.6099 mg / 100g GAE, tannin = 60.0496 mg / 100g TAE which has the potential as an antioxidant with an IC50 value of 81.811 mg / L which is included in the strong antioxidant category, and has various types of active compounds, namely the nonpolar fraction contains 53 compounds and the polar fraction contains 46 compounds activity and the level of public preference for So'e tangerine peel tea was higher in refined tea than coarse tea. Keywords: Citrus reticulata, phytochemicals, DPPH, GC-MS, traditional tea.
PERBANYAKAN ANGGREK Dendrobium heterocarpum Lindl. SECARA IN VITRO DENGAN MEDIA YANG BERBEDA Yuli Setiawati; Ida Ayu Astarini; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dendrobium heterocarpum Lindl. is one of the orchid species which is often seeked. It is because this species has a mystique appearance and special fragrance. The research aimed to observe the growth and developing responses of protocorm like bodies (PLBs) of D. heterocarpum after 12 weeks in three different medium, i.e. Murashige and Skoog (MS), Kursor C and Western 3 (W3). The D. heterocarpum seeds were planted in MS, Kursor C and W3 medium. Qualitatively the orchid growth were observe including growth score and colour score. The result showed that growing time of MS was 4 weeks, a week faster than the growing time on Kursor C and W3 medium. The growing phase on MS medium and Kursor C medium reached phase 1 whereas on W3 medium reached phase 2. PLB’s colour after 12 weeks on MS medium reached phase E, strong yellowish green phase (142A). Meanwhile, on Kursor C and W3 was on phase D, moderate yellowish green (143D).
AKTIVITAS ANTIOKSIDAN EKSTRAK TUMBUHAN PAKU EKOR KUDA (Equisetum debile L.) TERHADAP PEROKSIDASI LIPID PLASMA DARAH MENCIT (Mus musculus) Riana Dyah Suryaningrum; Ni Made Puspawati; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p08

Abstract

The purpose of this research was to study the antioxidant activity from ethanol extract of horstail (Equisetum debile L.) in the free radical scavenging in mice blood plasma. The antioxidant activity test was conducted with the DPPH method and measuring the MDA concentration in mice blood. The antioxidant activity test with the DPPH results showed that the ethanol extract of horstail (Equisetum debile L.) had the antioxidant activity of IC50 which was 1.604 mg/mL or 1,604 ppm. The statistical analysis result of the MDA blood plasma in mice with various doses (125 mg/kgBM, 250 mg/kgBM, 375 mg/kgBM and 500 mg/kgBM) showed that the 500 mg/kgBM dose extract was able to reduce the MDA concentration in mice blood which given the most amount of exercise.
EFEKTIVITAS EKSTRAK KULIT BATANG AMPUPU (Eucalyptus alba Reinw. Ex. Blume) DALAM MENGHAMBAT PERTUMBUHAN JAMUR Fusarium sp. PENYEBAB BUSUK TONGKOL JAGUNG (Zea mays L.) Bernadina Metboki; Ni Putu Adriani Astiti; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p01

Abstract

The fungal-related diseases were responsible as one of many reasons for low production of maize in Indonesia. Controlling fungal contaminants using chemical is hazardous for human nowadays. Thus the need of environmental friendly vegetative-based fungicide such as from the extract of Ampupu tree bark is important. The objective of this study was to investigate the antifungal effect from Ampupu tree bark crude extract in response to growth of Fusarium sp, which responsible as the causal agent of corncob rot. The inhibition test on growth of Fusarium sp. by Ampupu tree bark crude extract was performed with diffusion well method on PDA as media. The process involved the infusion of crude extracts at 1.5%, 3.0% and 4.5% concentration, respectively, into diffusion well on each Petri disk. The result showed that by in vivo treatment, crude extract of Ampupu tree bark with concentration of 1.5%, 3,0% and 4.5%, were able to inhibit the fungal growth of Fusarium miniliforme as the causal agent of corncob rot with diameter of inhibition zone 0.18 mm, 1.85 mm and 2.01 mm, respectively. The higher the concentration of the crude extract given, the larger the diameter of inhibition zone formed.
Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Bernadina Metboki Deglory Tunmuni Dwi Ariani Yulihastuti ENIEK KRISWIYANTI I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Suada IDA AYU ASTARINI Ida Ayu Meva Lianawati Ida Ayu Nopiari Ida Bagus Gede Darmayasa IRIANI SETYAWATI Jeremy Pramana Kadek Ayu Trisna Yanti Lilis Riana Tambunan Luh Putu Wrasiati MADE PHARMAWATI Made Ria Defiani Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Kade Ayu Purnama Adi Ni Komang Tia Pramesti Ni Luh Suriani Ni Made Dewi Wahyuni Ni Made Puspawati Ni Made Sastriyani Wiendra Ni Nyoman Nila Arieswari NI PUTU SRI PUSPITA WIDI YANTHI Ni Wayan Sudatri Pande Putu Purwaningsih Putu Yayun Antari Budaya Retno Kawuri Riana Dyah Suryaningrum Royana Pakpahan SANG KETUT SUDIRGA SARMILA TASNIM Yan Ramona Yuli Setiawati Yulidia Iriani Yulidia Iriani Yully Muharyati