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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology Bumi Lestari ISM (Intisari Sains Medis) : Jurnal Kedokteran

Ni Putu Adriani Astiti

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 220296

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Medicine & Pharmacology

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Articles

GC-MS Analysis of Active and Applicable Compounds in Methanol Extract of Sweet Star Fruit (Averrhoa carambola L.) Leaves Ni Putu Adriani Astiti; Yan Ramona
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.12

The leaves of star fruit plants (Averrhoa carambola) have been traditionally used to cure many diseases, such as hypertension and fever. Besides, the leaves of this plant have also widely been used as the main raw material in lawar (a Balinese traditional food) making. In this research, the methanol extract of this plant leaves was analyzed and identified for active compound contents. The main objective of this research was to investigate types of possible active and applicable compounds contained in such leaves, previously extracted/macerated in methanol solution. Analysis was conducted by applying GC-MS instrumentation using methanol solution. The results showed that 10 possible active and applicable compounds (Butane, 1,1-diethoxy-3 methyl-(CAS)1.1-DII, Dodecanoic acid, methyk ester(CAS) methyl, Dodecanoic acid, methyl ester (CAS) Ethyl Laun, Pentadecanoic acid ethyl ester, Hexadecanoic acid methyl ester (CAS) Methyl pa, OCTADECA 9.12 DIENOIC ACID METHYL, 9-Octadecenoic acid methyl ester (E)-(CAS), Octadecanoic acid methyl ester, and (E) 9-Octadecanoic acid ethyl ester) were identified in methanol extract of such leaves. Among those compounds, Butane, 1,1-diethoxy-3-methyl and Hexadecanoic acid, methyl ester was two most abundance constituents with percentage of peak areas of 35.67% and 26.93%, respectively.

IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (Vitis vinifera L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION Ni Nyoman Nila Arieswari; Ida Ayu Astarini; Ni Putu Adriani Astiti; Jeremy Pramana
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.

JENIS, STATUS DAN PEMANFAATAN TUMBUHAN JENIS POHON DI DESA ADAT BATURNING, KECAMATAN ABIANSEMAL, KABUPATEN BADUNG, BALI N. P. Adriani Astiti; Retno Kawuri; I K. Ginantra
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

There were 65 species of trees have been identified in Baturning village, District of Abiansemal, Regency of Badung, Bali, which were spread out around the river bank, cemetery (around Dalem temple), around the settlement, non-irrigated farm (plantation or “tegalan”) and rice field. Thirty eight (38) % out of them were observed to be available around the settlement. Most of trees in Baturning (61 species or 94%) have been used by the local community. The rest were uncultivated. From the use, trees in Baturning most were used for human consumption (24%). On the village has not been found protected species, even though in the village were found 22 species of trees which considered to be rare in Bali, namely: Bayur, Bentenung, Beringin, Cempaka, Ee, Jaka, Jeruk Bali, Juwet, Kelor, Kendal, Klampuak, Lempeni, Lenggung, Mahoni, Mengkudu, Palem Raja, Poh-Pohan, Pule, Sandat, Sentul, Udu, and Wani.

EFEKTIVITAS EKSTRAK DAUN JATI ( TECTONA GRANDIS L. F) DALAM MENGHAMBAT PERTUMBUHAN JAMUR HORMISCIUM Sp. Ni Putu Adriani Astiti
Bumi Lestari Journal of Environment Vol 15 No 1 (2015)
Publisher : Environmental Research Center (PPLH) of Udayana University

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A research on effectiveness of extracts of teak (Tectona grandis L. f) leaves in inhibit thegrowth of a species of fungi, Hormiscium sp. has been carried out. The leaves used in thisresearch were young leaves (number 1 and 2), and old ones (number 5 and 6), with fourconcentrations: 0, 2, 5, and 10 %. The media utilised were bean sprouts agar, and thereplications was 3 times. Qualitative tests were undertaken for the flavonoids, while alkaloidtests were based on Culvenor Fitzgerald methods. Qualitative tests of triterpenoids andsteroids were undertaken by utilising Lieberman – Burchard reagents (L-B reagents).Parameters observed were colony diameters, micellium weights (dry mass), and numbers ofconidia (ml). Results of this research showed that extracts of teak (T. grandis) leaves wereable to inhibit the growth of Hormiscium sp. The capability of inhibitions was increased asthe extract concentrations increased (for both, that of the young and old leaves), eventhough extracts of the young provided a higher capability of inhibitions compared to theold ones.

Efektifitas daun salam dalam mengurangi cemaran mikroba penyebab busuk telur itik Yulidia Iriani; Yan Ramona; Ni Putu Adriani Astiti
Intisari Sains Medis Vol. 13 No. 2 (2022): (In Press : 1 August 2022)
Publisher : DiscoverSys Inc.

Background: Pathogenic bacteria on eggshells or egg contents will be very dangerous for the health of humans who consume them. Chemicals should not be an option for preservatives because they will cause side effects. In this study, we explored the possibility of using bay leaf extract (Syzygium polyanthum (Wight) Walp) to control the growth of polluting bacteria in salted duck eggs so that the shelf life can be extended. The purpose of the study was to determine the potential of the active compounds contained in bay leaves in reducing the total plate count/microbial contamination in salted duck eggs. Methods: Extraction of bay leaf powder was carried out by the infusion method. The activity test of bay leaf decoction (ARDS) was carried out using the in vitro diffusion well method on Mueller Hinton media. Identification of active compounds was carried out using the GCMS technique. Results: ARDS crude extract at a concentration of 5% (v/v) could significantly reduce ALT and inhibit the in vitro growth of Proteus mirabilis bacteria at a concentration of 10% (w/v), with an inhibition zone diameter of 19.53 mm, with the minimum value inhibitory concentration (MIC) 0.7%. The results of the GCMS analysis of ARDS showed that the ten peaks produced, possibly synergized, and had antimicrobial activity. These compounds are 2-Pentanone4-Hydroxy-4 Methyl (CAS) Diacetone Alcohol; Ethanol, 2-(Ethoxythoxy) (CAS); 1,2,3 Propanetrriol Cas) Glycerol; Citronella (2,6-Octadien-1-Ol, 3,7-Dimethyl, (CAS); Beta Citronellol 6-Octen-1-Ol, 3,7 Dimethyl-(CAS); Trans Geraniol; Dodecanoic Acid, Methyl Ester (CAS) Methyl Laurate; Tetradecanoic Acid, Methyl Ester (CAS) Methyl Myristate; 1-Tetradecanol (Cas) Alfol 14 and Hexadecanoic Acid Methyl Ester CAS) Methyl Palmitate Conclusion: Leaf decoction can inhibit the growth of Proteus mirabilis in vitro, and the addition of ARDS with a concentration of 5% (v/v) can significantly reduce the value of bacterial contamination in salted duck eggs. Latar Belakang: Bakteri pathogen pada kulit telur atau isi telur akan sangat membahayakan kesehatan manusia yang mengkonsumsinya. Bahan kimia seharusnya tidak menjadi pilihan untuk pengawet, karena akan menimbulkan efek samping, Pada penelitian ini dieksplorasi, kemungkinan dipakainya ekstrak daun salam (Syzygium polyanthum (Wight) Walp) untuk mengontrol pertumbuhan bakteri pencemar pada telur itik yang diasinkan, sehingga masa simpannya dapat diperpanjang. Tujuan penelitian untuk mengetahui potensi senyawa aktif yang terdapat pada daun salam dalam menurunkan angka lempeng total/cemaran mikroba pada telur itik yang diasinkan. Metode: Ekstraksi serbuk daun salam dilakukan dengan metode infusa. Uji aktifitas air rebusan daun salam (ARDS) dilakukan dengan metode sumur difusi secara in vitro pada media Mueller Hinton. Identifikasi senyawa aktif dilakukan dengan teknik GCMS. Hasil: Ekstrak kasar ARDS pada konsentrasi 5% (v/v) secara nyata dapat menurunkan ALT dan menghambat pertumbuhan in vitro bakteri Proteus mirabilis pada konsentrasi 10% (b/v), dengan diameter zona hambat sebesar 19,53 mm, nilai minimum inhibitory concentration (MIC) 0,7%. Hasil analisis GCMS terhadap ARDS menunjukan bahwa sepuluh puncak yang dihasilkan, kemungkinan bersinergi dan mempunyai aktifitas sebagai antimikroba. Senyawa-senyawa tersebut adalah 2-Pentanone4-Hydroxi-4 Methyl (CAS) Diacetone Alkohol; Ethanol, 2-(Ethoxythoxy) (CAS); 1,2,3 Propanetrriol Cas) Glycerol; Citronella (2,6-Octadien-1-Ol, 3,7-Dimethyl, (CAS); Beta Citronellol 6-Octen-1-Ol, 3,7 Dimethyl-(CAS); Trans Geraniol; Dodecanoic Acid, Methyl Ester(CAS) Methyl Laurate; Tetradecanoic Acid,Methyl Ester (CAS) Methyl Myristate; 1-Tetradecanol (Cas) Alfol 14 dan Hexadecanoic Acid Methyl Ester CAS) Methyl Palmitate. Simpulan: Air rebusan daun dapat menghambat pertumbuhan Proteus mirablis secara in vitro dan penambahan ARDS dengan konsentrasi 5% (v/v) secara signifikan dapat menurunkan nilai cemaran bakteri (angka lempeng total) pada telur itik yang diasinkan.

Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Bernadina Metboki Deglory Tunmuni Dwi Ariani Yulihastuti ENIEK KRISWIYANTI I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Suada IDA AYU ASTARINI Ida Ayu Meva Lianawati Ida Ayu Nopiari Ida Bagus Gede Darmayasa IRIANI SETYAWATI Jeremy Pramana Kadek Ayu Trisna Yanti Lilis Riana Tambunan Luh Putu Wrasiati MADE PHARMAWATI Made Ria Defiani Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Kade Ayu Purnama Adi Ni Komang Tia Pramesti Ni Luh Suriani Ni Made Dewi Wahyuni Ni Made Puspawati Ni Made Sastriyani Wiendra Ni Nyoman Nila Arieswari NI PUTU SRI PUSPITA WIDI YANTHI Ni Wayan Sudatri Pande Putu Purwaningsih Putu Yayun Antari Budaya Retno Kawuri Riana Dyah Suryaningrum Royana Pakpahan SANG KETUT SUDIRGA SARMILA TASNIM Yan Ramona Yuli Setiawati Yulidia Iriani Yulidia Iriani Yully Muharyati

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