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ANALISIS FAKTOR TITIK KRITIS DAN UJI MALACHITE GREEN UNTUK MENENTUKAN STATUS HALAL AYAM POTONG DI TPA KECAMATAN MENES Marlinda Indriati; Tuti Rostianti Maulani; Uti Nurliawati
International Journal Mathla’ul Anwar of Halal Issues Vol. 1 No. 1 (2021)
Publisher : Universitas Mathla’ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.189 KB) | DOI: 10.30653/ijma.202111.6

Abstract

This study aims to analyze the critical point factor and malachite green test to determine the halal status of broiler chickens in chicken slaughterhouses (TPA). Samples were taken from 4 landfills in Menes District. The research was conducted in 3 stages, namely first filling the halal slaughtering quisoner according to the LPPOM MUI standard (2011), with results of 80% in accordance with the halal chicken slaughtering technique. The second stage is the Malachite Green test, which aims to prove whether the process of slaughtering broiler chickens is perfect, seen from the removal of chicken blood which must also be perfect, the data obtained is that blood removal is carried out completely from all samples (negative carcass). The last stage is post-slaughter handling by testing Eacherichia coli microbial contamination. The average value of microbial contamination is 2.6 x 104 cfu / gr with purple colonies on brilliance media, and the amount of e.coli contamination of broiler chicken meat exceeds the maximum limit of Eacherichia Coli microbial contamination (BMCM) of fresh chicken meat is less than 1 x 101 cfu / gr.
Deteksi Cemaran DNA Babi Dengan RT-PCR Pada Sosis Tanpa Logo Halal MUI Dari Empat Kecamatan di Kabupaten Pandeglang Banten Tuti Rostianti Maulani; Hadi Susilo; Marlinda Indriati; A Suhaemi
Gorontalo Agriculture Technology Journal Volume 3 Nomor 2 Oktober 2020
Publisher : Universitas Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.611 KB) | DOI: 10.32662/gatj.v3i2.1171

Abstract

Sosis merupakan produk olahan daging yang cukup digemari di masyarakat khususnya di Kabupaten Pandeglang Provinsi Banten.   Pentingnya sertifikat halal atau adanya logo halal MUI (Majelis Ulama Indonesia) untuk produk olahan daging membuat masyarakat muslim yakin untuk mengkonsumsinya.  Tujuan dari penelitian ini adalah identifikasi cemaran DNA babi  pada produk sosis yang beredar di 4 kecamatan di Wilayah Kabupaten Pandeglang tanpa logo halal MUI menggunakan RT-PCR (Real Time- Polymerase Chain Reaction) dan Pork Detection Kit.  Metode dalam penelitan ini diawali dengan menggunakan Pork Detection Kit untuk pengujian antigen babi sebagai kontrol positif dan daging sapi sebagai kontrol negatif yang akan digunakan untuk running RT- PCR. Hasil running RT- PCR terhadap sampel DNA sosis di wilayah 4 (empat) kecamatan di Kabupaten Pandeglang (Majasari, Picung, Munjul, Cimanuk) tanpa label halal MUI menunjukkan trend DNA kontrol negatif pada kurva amplifaksi RT- PCR. Hal ini teridentifikasi bahwa sampel DNA sosis tanpa label halal MUI di 4 kecamatan di Kabupaten Pandeglang tidak tercemar DNA babi