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All Journal Aquacultura Indonesiana Journal of Tropical Pharmacy and Chemistry
M. Farid Rahman
Chemistry Department, Faculty of Mathematics and Sciences, Brawijaya University, Jl. Veteran, Malang
Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology

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Isolation and Identification of Jellyfish Alkaloid (Bougainvillia Sp) as Immunostimulant to Profile of Protein and Fagosite Activity of Tiger Grouper (Epinephelus Fuscoguttatus) Andayani, Sri; Fadjar, M.; Rahman, M. Farid
Aquacultura Indonesiana Vol 18, No 2 (2017)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.667 KB) | DOI: 10.21534/ai.v18i2.113

Abstract

The aim of this research was to know the profil protein and phagocyte activity of tiger grouper (Epinephelus fuscoguttatus). The method used in this research was experimental method with Completely Randomized Design (RAL) consisting of 5 treatment levels ie. K=control group without giving alkaloids and feeding pellets mixed with Jellyfish alkaloid Bougainvillia sp., which were treatment A = 0.5 g, B = 0.75 g, C= 1 g and D = 1.25 g alkaloid/kg of feed conducted in 2 replications. It was challenge tested with Vibrio harveyi of 107 cfu/mL for 5 days. Blood plasma for protein profile and phagocyte activity was performed after giving immunostimulant on day 28 and after being infected on day 34. The results of the study were: (1) alkaloid characteristic was N-1 Benzylalcohol, 4-Octyl Piperidine) and (2) protein profile after immunostimulant addition resulted in 9  bands and sample after infection showed 7 protein bands. The phagocyte activity increases 11.4% to 80.6%. It is suggested to use 1 g of alkaloid/kg of feed as immunostimulant.
Screening of Aroma Compounds in Commercial Tea (Camellia sinensis) from Indonesia and Testing Their Activity as Antioxidants Warsito, Warsito; Alief, M. Fadel; Azzahra, Vina Octavia; Rahman, M. Farid; Retnowati, Rurini
Journal of Tropical Pharmacy and Chemistry Vol. 8 No. 2 (2024): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

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Abstract

Tea from tea leaves of the Camellia sinensis species is a superior industrial plant that promises superior bioactivity. For a more in-depth study of the essential oils contained in commercial teas on the market, their significant characteristic from their constituent compounds were determined. The essential oil yields of the three commercial samples were obtained by distillation using a Liken-Nickerson vessel and analyzed by gas chromatography-mass spectrometry (GC-MS), then tested for antioxidant properties by the DPPH method. The yield obtained ranged from 0.08-0.13%. The main major components (more than 0.5% identified) contained in the three samples identified were benzyl acetate, between 4,56-26.27%, and linalool compounds from tea leaves, as much as 20.59%, and 3.42% in samples C and B which were not found in sample A. As shown by our results, antioxidant activity significantly correlated with benzyl acetate and linalool composition. Sample A showed the best antioxidant profile, with highest inhibition (IC50 20.19 mg/mL).
Co-Authors Alief, M. Fadel Azzahra, Vina Octavia Fadjar, M. Rurini Retnowati Sri Andayani Warsito Warsito