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KARAKTER RESPIRASI DAN MINERALISASI KARBON ORGANIK PADA SAMPEL TANAH DIKOLEKSI DARI PULAU BANGKA [Respiration and Organic Carbon Mineralization Character in Soil Samples Collected from Bangka Island] Rahmansyah, Maman; Suliasih, Suliasih
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study was designed to explore soil biomass content and soil enzymatic activities that involved in carbon organic soil mineralization. Samples of soil were collected from two locations in Bangka Island. Bulk samples of top soil (TP) and excavated (TG) Â were collected from garden soil in Pangkalpinang. Other soils were gathered from forest floor (TU), beneath pelawan (Tristaniopsis merguensis Griff.) trees which is endemic plant to North Bangka forest. Soil biological character was evaluated by measuring soil microbial population, respiration rate, and cellulase and amylase activities. Experiments were carried out in Research Center for Biology, Indonesian Institute of Sciences, on dried soil treatment (Control), soil moistened with 5 mL H2O in 100g soil (Gluc.0), and soil moistened with 5 mL 1% glucose solution (Gluc.1). Bacterial population obtained was higher than fungal population in all soil samples. Soil respiration activity in TU after treatment was 4.51Â±0.49; 5.26Â±0,79; and 8.28Â±1.41 ppm CO2/100g-soil/5-minutes, respectively. Meanwhile , respiration in TP Â were 3.65 Â± 2.12; 3.57 Â± 1.18; and 7.94 Â± 1.05 ppm CO2/100g-soil/5-minutes; and in the TG are 2.61 Â± 0.70; 3.34 Â± 0.94; and 5.46 Â± 2.30 ppm CO2/100gsoil/5-minutes. Cellulase activity of all samples were not significantly different. Forest soil cellulase activity was significantly different compared to garden soil. Glucose induction was positively increased amylase activity compared to cellulase. Soil enzyme, soil respiration activities and microbial population of forest soil could be used as a reference to recover degraded land in Bangka Island.

PEMANFAATAN INOKULAN MIKROBA SEBAGAI PENGKAYA KOMPOS PADA BUDIDAYA SAYURAN [Microbial inoculants for compost enrichment on vegetables cultivation] Antonius, Sarjiya; Rahmansyah, Maman; Muslichah, Dwi Agustiyani
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Â Microbial existence is due to mineralize organic compound in soil and caused nutrients availability to the plants. In this study, some of microbial inoculants consist of a). Phosphate soluble bacteria (Pseudomonas sp.) collected from soils that intensively utilized agrochemical in its agriculture activities; b). Nitrogen - fixing bacteria Rhizobium leguminosarum; and c) Trichoderma sp. and Aspergillus sp.as organic degradation fungi were used for compost enrichment.These inoculants formulation were utilized to carrots (Daucus carota L.), broccoli (Brassica oleracea L.) and maize (Zea mays L.) cultivation in upland area, Cisarua, Bogor, West Java. This study was aimed to assess inoculants response to enrich fully mature and half ripe compost. Crops response to the inoculation treatment was varied among these commodities. In general, the response of plants with the use of compost enriched biofertilizer was positive, with an increase in yield of about 15- 30% for carrots, broccoli around 65- 90% and about 10% of corn crops. The significant increase in crops production as impact of organic fertilizier application indicated that C organic content of the organic farm of Cimelati and Agato were defisit.

PERFORMA BAKTERIPADATANAH TERCEMAR PESTISIDA Rahmansyah, Maman; Sulistinah, Nunik
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Preliminary study on bacterial survive in soil containing pesticide has been carried out. Soil samples collected from Lembang and Dieng. The soil deprive from agriculture area that intensively using pesticide, and compared to other samples gathered from forest soil. All samples examined for total bacteria, denitrification bacteria, phosphate solubilizing bacteria, soil induce respiration, urease and phosphatase activities. Pattern of whole parameters in each soil sample configured similarly, but the value performed differently in the same parameters. Total bacterial population in soil samples also inspected after the samples amended with some certain pesticides. Survival bacteria subjected to media amended with insecticide (Propoxur, Diazinon, and Chlorpyrifos), and herbicides (Bromacil and 2,4-D), and correlation of bacterial growth between sample location were varied. Bacterial degrading pesticide particularly isolated from the soil samples containing 1000 ppm Curzate (fungiside) and 500 ppm 2,4-D.The isolates then cultured in the medium containing insecticide and herbicide, and the response on growth observed in 7 days incubation. Bacterial perform were meaningful to reference of soil degrading pesticide residue in agriculture soil; and it would make representative reference in an effort to use bacteria throughout biofertilizer improvement.

AKTIVITAS FOSFATASE TANAH DILINGKUNGAN BENTANG HUTAN ALAMI DAN NON-ALAMI Suliasih, Suliasih; Rahmansyah, Maman
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The spatial dispersal of acid and alkaline phosphates activity in forest soils appears to be controlled by position in the landscape and its soil microbial density. Soil bacteria expressively produced significant level of acid phosphatase in the investigation.The variety of acid phosphatase activity (8.25-37.55 />-nitrophenol.g"soil.h") noted higher and followed by alkaline (0.78-7.15 p-nitrophenol.g-soil.h" ), correspondingly. Acid and alkaline phosphates were exist in both of soil of natural as well as for non-natural forest soil landscape, with the value (po/o=0.6210) of correlation are 0.6889 and 0.6532, respectively. Phosphate Solubilizing Bacteria (PSB) average density in natural forest soil equivalent to 0.61xl0Ã colony forming unit (cfu) and total bacteria is 160xl0 cfu, while the PSB in non-natural forest soil is 7.75xl0 cfu and total bacteria is 48.25x10 cfu. Concerning to the forest environment as soil bacteria inhabitants of PSB, there were significantly difference of PSB population in natural and non-natural landscape forest. Forest environment is negatively affect the soil enzymes activities under Pinus maritima and Caliandra calothyrsus as the lowest activities, and to the highest ones under the Schima wallichii and Eucalyptus saligna vegetation.

CELLULOLYTIC BACTERIA OF SOIL OF GUNUNG HALIMUN NATIONAL PARK Sudiana, I Made; Rahayu, Ritai Dwi; Imanuddin, Hartati; Rahmansyah, Maman
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1077

The population of aerobic cellulolyitic bacteria (ACB) of soil Gunung Halimun National park and its celulolytic capacity were studied.The soil samples were collected from various altitude (500-1500) m asl.Microbial isolation was performed by culture enrichment technique with CMC (carboxymethyl cellulose) as the major carbon sources.The quantitative determination of ACB was performed by growing the microbes on CMC containing media, and utilizing congored as an indicator.ACB was indicated by formation of clearing zone surrounding growing colony.Cellulytic capacity of each isolates was determined by analysing the ratio of colony and clear zone formation.ACB were quite heterogenous include Bacillus sp., Clostridium sp., Chromobacterlum sp., Enterobacter sp., Moraxella sp. and Pseudomonas sp.

PERBANDINGAN POLA ROMBAK SELULOSA OLEH BEBERAPA JAMUR BASIDIOMYCETES RAHMANSYAH, MAMAN
BERITA BIOLOGI Vol 3, No 9 (1989)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

MAMAN RAHMANSYAH, 1989,Comparative capability of cellulose degradation by Basidiomy cetes fungi.Berita Biologi 3(9) 450 - 453.Cellulosa is natural resource that still found abandon and its can be composted by themselves naturaly to the earth.Basidiomycetes fungi offered to convert those waste organic matter into useful product.Four Basidiomycetes fungi (Auricularia polytricha, Coprinus cinereus, Pleurotus qstreatus and Volvariella valcacea) have been tested to degrade riee straw, Whatman filter paper and cellulose powder. Three replicates of 3 grams of media were inoculated by different fungus land were fermented for 21 days at room temperature (± 28 C). Biodegradation rare was measured by the lost weight basis of media and bioconvertion rate was detected by stating ability of enzime (cellulose), which were extracted from cellulose powder culture,to convert CMC substrat and glucose production.The result shows that C. cinereus has almost 3 to 4 times degrading and converting capability compared to other fungi.

PENGARUH PERLAKUAN AWAL TERHADAP BENIH TOMAT (LYCOPERSICON ESCULENTUM MILL.) RAHMANSYAH, MAMAN
BERITA BIOLOGI Vol 3, No 9 (1989)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i9.1301

MAMAN RAHMANSYAH,1978.Effect of preconditioning treatments on tomato seeds.Berita Biologi 3(9): 473-4 75. The study the effect of preconditioning treatments for tomato seeds on plant growth and production,five thousand tomato seeds were slowly rehydrated from 6 to 12% moisture content.The treated seeds were sown on moist straw paper at low (26°-28°C)and high (26°- 36°C fluctuated temperatures,others were planted after storage for 48 and 60 hours in the in-cubator (40 C).The germination percentages of seeds,pollen fertility, the percentage of fruits developed, the average weight of fruit and the weight of 1000 seeds were evaluated.Plants derived from rehydrating seeds and stored for 48 hours at 40°C gave the best performance on their germination (more than 90%),pollen fertility (95%) and also the highest total fruits harvested but not on fruit and seed weights.The best result of seed treatment were 12% of moisture content and its were stored for 48 hours at 40°C

PERBANDINGAN POLA ROMBAK SELULOSA OLEH BEBERAPA JAMUR BASIDIOMYCETES MAMAN RAHMANSYAH
BERITA BIOLOGI Vol 3, No 9 (1989)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i9.1295

MAMAN RAHMANSYAH, 1989,Comparative capability of cellulose degradation by Basidiomy cetes fungi.Berita Biologi 3(9) 450 - 453.Cellulosa is natural resource that still found abandon and its can be composted by themselves naturaly to the earth.Basidiomycetes fungi offered to convert those waste organic matter into useful product.Four Basidiomycetes fungi (Auricularia polytricha, Coprinus cinereus, Pleurotus qstreatus and Volvariella valcacea) have been tested to degrade riee straw, Whatman filter paper and cellulose powder. Three replicates of 3 grams of media were inoculated by different fungus land were fermented for 21 days at room temperature (± 28 C). Biodegradation rare was measured by the lost weight basis of media and bioconvertion rate was detected by stating ability of enzime (cellulose), which were extracted from cellulose powder culture,to convert CMC substrat and glucose production.The result shows that C. cinereus has almost 3 to 4 times degrading and converting capability compared to other fungi.

PENGARUH PERLAKUAN AWAL TERHADAP BENIH TOMAT (LYCOPERSICON ESCULENTUM Mill.) MAMAN RAHMANSYAH
BERITA BIOLOGI Vol 3, No 9 (1989)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i9.1301

MAMAN RAHMANSYAH,1978.Effect of preconditioning treatments on tomato seeds.Berita Biologi 3(9): 473-4 75. The study the effect of preconditioning treatments for tomato seeds on plant growth and production,five thousand tomato seeds were slowly rehydrated from 6 to 12% moisture content.The treated seeds were sown on moist straw paper at low (26°-28°C)and high (26°- 36°C fluctuated temperatures,others were planted after storage for 48 and 60 hours in the in-cubator (40 C).The germination percentages of seeds,pollen fertility, the percentage of fruits developed, the average weight of fruit and the weight of 1000 seeds were evaluated.Plants derived from rehydrating seeds and stored for 48 hours at 40°C gave the best performance on their germination (more than 90%),pollen fertility (95%) and also the highest total fruits harvested but not on fruit and seed weights.The best result of seed treatment were 12% of moisture content and its were stored for 48 hours at 40°C

CELLULOLYTIC BACTERIA OF SOIL OF GUNUNG HALIMUN NATIONAL PARK I Made Sudiana; Ritai Dwi Rahayu; Hartati Imanuddin; Maman Rahmansyah
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1077

The population of aerobic cellulolyitic bacteria (ACB) of soil Gunung Halimun National park and its celulolytic capacity were studied.The soil samples were collected from various altitude (500-1500) m asl.Microbial isolation was performed by culture enrichment technique with CMC (carboxymethyl cellulose) as the major carbon sources.The quantitative determination of ACB was performed by growing the microbes on CMC containing media, and utilizing congored as an indicator.ACB was indicated by formation of clearing zone surrounding growing colony.Cellulytic capacity of each isolates was determined by analysing the ratio of colony and clear zone formation.ACB were quite heterogenous include Bacillus sp., Clostridium sp., Chromobacterlum sp., Enterobacter sp., Moraxella sp. and Pseudomonas sp.

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