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Pseudozyma aphidis as inoculant for local chicken Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

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Pertumbuhan Bakteri Hasil Isolasi dari Tanah Perkebunan yang Tumbuh pada Media Mengandung Pestisida Propoksur dan Karbaril Imamuddin, Hartati; Laili, Nur; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Growth Capacity of Soil Bacteria Isolates Obtained from Estate Area from Media Containing Propoxur and Carbaryl. Isolation actions have been carried out with three soil samples derived from soil estate of pine apple, sugar cane, and banana in Lampung area, Southern Sumatra. The isolates were grown in the selected media containing carbaryl insecticide, and herbicide of diuron and bromocyl. The highest and vigorous growth isolates have gathered from threedifferent soil samples, and subsequently named GGPC, GM, and NTF isolates. Furthermore, those isolates were studied through the growth rate in the media containing propoxur and carbaryl in the nutrient rich liquid (NB) and compared to limited mineral (MM). All of the isolates grew faster against pesticides in the liquid medium of NB compared to MM. High correlated value among parameters nurtured as due to bacterial population and those processes were not affected by media composition. The GM isolate showed similar pattern during propoxur decrease along with incubation even though the rate of pesticide elimination from the media was higher in the rich nutrient liquid media (NB) compared to MM.Keywords: microbe isolate, pesticide, propoxur, carbaryl

Pengaruh Pemberian Kompos dan Pupuk Hayati terhadap Aktivitas Fosfatase dan Urease pada Tanah yang Ditanami Brokoli (Brassica oleracea L.) Rahmansyah, Maman; Antonius, Sarjiya
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2163

Organic farming basically work on improving organic soil substrate which give impact to plant growth promoting bacteria. On the other hand, the use of agrochemicals leave chemical residues in the soil. In an effort to get the quality of organic matter and selected microbes as a biofertilizer material, this research had been done. Half ripe composting (HRC) process of organic substrate was utilized to enrich soil containing agrochemical residue, while ripe compost (RC) substance amended to soil free residue. That compost was used in broccoli cultivation. Microbial degrading pesticide was added to compost as biofertilizer formula. Even though plant biomass and the yield of flower was unclearly affected, but moreover the HRC application as due to Ã¢â¬Åsemi-on-site-compostingÃ¢â¬Â practices in soil containing agrochemical residue would cut off compost processing time. Soil microbial activity measured through soil respiration, urease and phosphomonoesterase indicated that the value was lower in samples collected from the soil free residue compared with soils containing residues. Utilization of chicken manure resulted in the availability of high nitrogen compounds and causing imbalance absorption by plants, compared with the use of HRC and RC in this study.ÃÂ Keywords: soil respiration, phosphatase, urease, Brassica oleracea L.ÃÂ

Optimasi Enzim ?-Amilase dari Bacillus amyloliquefaciens O1 yang Diinduksi Substrat Dedak Padi dan Karboksimetilselulosa Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Â ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media. Â The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth.Â Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcelluloseÂ

Production of Acid Phosphatase in Bacillus sp. Isolated from Forest Soil of Gunung Salak National Park Rahmansyah, Maman; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3140

ABSTRAKProduktivitas Fosfatase Asam pada Bacillus sp. yang Diisolasi dari Tanah Hutan TamanNasional Gunung Salak. Pada pengamatan ini dilakukan karakterisrik bakteri pelarut fosfatyang diisolasi dari tanah hutan Taman Nasional Gunung Salak. Sebanyak 21 koloni hasil isolasidiuji terhadap produktivitas enzim fosfatase berdasar pelarutan media mengandung fosfat.Isolat yang terkuat melarutkan fosfat diidentifikasi sebagai Bacillus sp. Pada pengamatanlanjutan terhadap strain teruji dilakukan penumbuhan pada media cair selama 90 jam inkubasi,dan hasilnya ternyata mampu melarutkan fosfat inorganik (Pi) dari sumber trikalsium fosfat(Ca-Pi) dan alumunium fosfat (Al-Pi) masing-masing pada kisaran 1,2 sampai 152 dan 0.8 sampai25 mg.L-1; dan menunjukkan aktifitas enzim fosfomonoesterase antara 0.2 sampai 1.01 unitpada media yang mengandung larutan para-nitrophenylphosphate sebagai media fosfatorganik (Po) artifisial. Konsumsi glukosa pada media yang diukur selama pertumbuhan sejalanpula dengan produk ortofosfat sebagai akibat adanya aktifitas enzim fosfatase. Peningkatanfosfatase juga sejalan dengan bertambahnya biomassa sel bakteri dan penambahan produkasam glukonat. Penurunan pH dari 7 menjadi 5 diakibatkan peningkatan produk asam glukonatdi dalam media tumbuh. Bakteri pelarut fosfat yang berasal dari tanah hutan Taman NasionalGunung Salak dapat memproduksi fosfatase asam untuk memineralisasi sumber-sumber fosfatmenjadi sumber nutrisi yang siap digunakan oleh akar tumbuhan, dan itu merupakan prediksikuat untuk menjadikan isolat bakteri pelarut fosfat sebagai sumber bahan pupuk hayati.Kata kunci: Bacillus sp., tanah hutan, fosfatase asam, Ca-Pi, Al-Pi.

Pengaruh Inokulasi Bakteri Terhadap Pertumbuhan Awal Jarak Pagar (Jatropha curcas L.) Widawati, Sri; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3175

ABSTRACTBacterial inoculants affect the early growth of Jatropha (Jatropha curcas L). Genera ofAzotobacter, Bacillus, Chromobacterium, Citrobacter, Nitrosomonas, Rhizobium, andSpaerotillus natans were soil bacterial isolates. The soil was collected from numerous placesaround Pontianak, West Kalimantan. Those isolates were used as inoculants, and formulatedto single and mixed bacterial inoculants, then used to stimulate the early growth of jatrophaseedling in 15 weeks at greenhouse condition. Bacterial inoculations caused better growthperformance compared to its control as pure soil garden medium without inoculations, andneither to bare soil dresses with compost. In the presence of inoculants, plant height wasaccelerated quickly while other inoculants affected to stalk diameter development. Daily growthperformance of jatropha peaked in 8 and 11 weeks after inoculation of Citrobacter andNitrosomonas bacterial component were used as single inoculant, respectively. The increasingof shoot biomass accumulation was three times as caused by single inoculants (Bacillus sp),and the highest one up to four times of biomass weight caused by a mixture inoculants asconsortium of Azotobacter, Bacillus, and Nitrosomonas spp. That selective inoculant hasopportunity to be used for jatropha farming, and this basic study is meaningful to jatropacultivation for standing to bio-fuel resources.Keywords: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.Kata kunci: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.

Ragam Aktivitas Urease dan Fosfomonoesterase serta Perannya dalam Ketersediaan Nutrisi N dan P pada Tanah Kebun Biologi Wamena Rahmansyah, Maman; Latupapua, H.J.D.; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTDiscrepancy of urease and phosphomonoesterase activities and its role in establishing Nand P nutrition in soil collected from Wamena Biological Research Station. Microbialactivities in soil lead to know for establishing soil nutrient status. Accordingly, soil collectedfrom Biological Research Station in Wamena then sent to the laboratory and determined on itsenzymatic activities and the physicochemical, as well. In this work, the enzymatic activities ofurease and phosphomonoesterase were examined in relation with soil microbial respiration, inorder to understand the mineralization of nitrogenous and phosphorus compound in soil. Soilrespiration rate (2.43-3.21 mg C02 g-dm12hour) designated variation in each sample, as wellas urease (8.6-23.5 unit/g soil) and phosphomonoesterase (5.5-7.9 unit/g soil) activities.Phosphomonoesterase activity showed strong correlation with respiration rate within soil; andreveal to the configuration of the bioactivities and physicochemical soil figures concluded thatthe B sample has the poor fertility. The phenomenon of data fulfill that bioactivities hadcorrelation with the physicochemical compound in the soil.Keywords: respiration, urease, phosphomonoesterase, Wamena Biological Research Station.

KARAKTER RESPIRASI DAN MINERALISASI KARBON ORGANIK PADA SAMPEL TANAH DIKOLEKSI DARI PULAU BANGKA [Respiration and Organic Carbon Mineralization Character in Soil Samples Collected from Bangka Island] Rahmansyah, Maman; Suliasih, Suliasih
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study was designed to explore soil biomass content and soil enzymatic activities that involved in carbon organic soil mineralization. Samples of soil were collected from two locations in Bangka Island. Bulk samples of top soil (TP) and excavated (TG) Â were collected from garden soil in Pangkalpinang. Other soils were gathered from forest floor (TU), beneath pelawan (Tristaniopsis merguensis Griff.) trees which is endemic plant to North Bangka forest. Soil biological character was evaluated by measuring soil microbial population, respiration rate, and cellulase and amylase activities. Experiments were carried out in Research Center for Biology, Indonesian Institute of Sciences, on dried soil treatment (Control), soil moistened with 5 mL H2O in 100g soil (Gluc.0), and soil moistened with 5 mL 1% glucose solution (Gluc.1). Bacterial population obtained was higher than fungal population in all soil samples. Soil respiration activity in TU after treatment was 4.51Â±0.49; 5.26Â±0,79; and 8.28Â±1.41 ppm CO2/100g-soil/5-minutes, respectively. Meanwhile , respiration in TP Â were 3.65 Â± 2.12; 3.57 Â± 1.18; and 7.94 Â± 1.05 ppm CO2/100g-soil/5-minutes; and in the TG are 2.61 Â± 0.70; 3.34 Â± 0.94; and 5.46 Â± 2.30 ppm CO2/100gsoil/5-minutes. Cellulase activity of all samples were not significantly different. Forest soil cellulase activity was significantly different compared to garden soil. Glucose induction was positively increased amylase activity compared to cellulase. Soil enzyme, soil respiration activities and microbial population of forest soil could be used as a reference to recover degraded land in Bangka Island.

PEMANFAATAN INOKULAN MIKROBA SEBAGAI PENGKAYA KOMPOS PADA BUDIDAYA SAYURAN [Microbial inoculants for compost enrichment on vegetables cultivation] Antonius, Sarjiya; Rahmansyah, Maman; Muslichah, Dwi Agustiyani
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Â Microbial existence is due to mineralize organic compound in soil and caused nutrients availability to the plants. In this study, some of microbial inoculants consist of a). Phosphate soluble bacteria (Pseudomonas sp.) collected from soils that intensively utilized agrochemical in its agriculture activities; b). Nitrogen - fixing bacteria Rhizobium leguminosarum; and c) Trichoderma sp. and Aspergillus sp.as organic degradation fungi were used for compost enrichment.These inoculants formulation were utilized to carrots (Daucus carota L.), broccoli (Brassica oleracea L.) and maize (Zea mays L.) cultivation in upland area, Cisarua, Bogor, West Java. This study was aimed to assess inoculants response to enrich fully mature and half ripe compost. Crops response to the inoculation treatment was varied among these commodities. In general, the response of plants with the use of compost enriched biofertilizer was positive, with an increase in yield of about 15- 30% for carrots, broccoli around 65- 90% and about 10% of corn crops. The significant increase in crops production as impact of organic fertilizier application indicated that C organic content of the organic farm of Cimelati and Agato were defisit.

PERFORMA BAKTERIPADATANAH TERCEMAR PESTISIDA Rahmansyah, Maman; Sulistinah, Nunik
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Preliminary study on bacterial survive in soil containing pesticide has been carried out. Soil samples collected from Lembang and Dieng. The soil deprive from agriculture area that intensively using pesticide, and compared to other samples gathered from forest soil. All samples examined for total bacteria, denitrification bacteria, phosphate solubilizing bacteria, soil induce respiration, urease and phosphatase activities. Pattern of whole parameters in each soil sample configured similarly, but the value performed differently in the same parameters. Total bacterial population in soil samples also inspected after the samples amended with some certain pesticides. Survival bacteria subjected to media amended with insecticide (Propoxur, Diazinon, and Chlorpyrifos), and herbicides (Bromacil and 2,4-D), and correlation of bacterial growth between sample location were varied. Bacterial degrading pesticide particularly isolated from the soil samples containing 1000 ppm Curzate (fungiside) and 500 ppm 2,4-D.The isolates then cultured in the medium containing insecticide and herbicide, and the response on growth observed in 7 days incubation. Bacterial perform were meaningful to reference of soil degrading pesticide residue in agriculture soil; and it would make representative reference in an effort to use bacteria throughout biofertilizer improvement.

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