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Sulistiani ., Sulistiani
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Religion Biochemistry, Genetics & Molecular Biology Education Languange, Linguistic, Communication & Media

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Variasi Intraspesies Lactobacillus plantarum (Orla-Jensen) Bergey et al. Asal Sayur Asin Berdasarkan Analisis Molekuler ., Sulistiani; ., Abinawanto; Sukara, Endang; Dinoto, Achmad; Mangunwardoyo, Wibowo
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2162

Abstract

The current study is the first report on intraspecies analysis of L. plantarum from sayur asin in Indonesia using molecular approach. Three molecular techniques, i.e., restriction fragment length polymorphism (RFLP) 16S-23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and enterobacterial repetitive intergenic consensus (ERIC-PCR) were used to determine the intraspecies diversity of L. plantarum responsible for spontaneous fermentation in sayur asin. These methods were aimed to discriminate 46 isolates of L. plantarum isolated from sayur asin, including the type strain. PCR amplification of the 16S-23S rDNA ISR revealed two-bands profile of 800 and 600 bp specific to lactobacilli. RAPD-PCR and ERIC-PCR were very valuable in discriminating genetic polymorphism among L. plantarum isolates by producing bands ranged from 4-10 bands (360-2620 bp) and 6-12 bands (160-2900 bp), respectively. Dendograms generated from UPGMA cluster analysis based on RAPD-PCR and ERIC-PCR data showed that all isolates were grouped into three major clusters with 74% and 68.6% genetic similarity thresholds, respectively.The study indicated that strains belong to L. plantarum isolated from sayur asin were divided into three genotypic groups. Keywords: ERIC-PCR, Intraspecies, Lactobacillus plantarum, RAPD-PCR, RFLP 16S-23S rDNA ISR 
Optimasi Enzim ?-Amilase dari Bacillus amyloliquefaciens O1 yang Diinduksi Substrat Dedak Padi dan Karboksimetilselulosa Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1523.768 KB) | DOI: 10.14203/jbi.v11i2.2200

Abstract

 ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media.  The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth. Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcellulose 
ROLE SCHOLARS SOCIETY FORM AND INTELLECTUAL MULTICULTURAL ., Sulistiani

Publisher : Pascasarjana PAI Multikultural Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.784 KB)

Abstract

Attitudes and behaviors that emerge are often unsympathetic, even very contrary to the cultural values of noble ancestors. Attitudes such as solidarity, respect for others, gotong royong began to fade. The arrogance of the majority culture as a result of the dominance lead to a lack of understanding the culture and interact with other people. Apart from the interests of both individuals and groups, often a dispute that not infrequently lead to disunity, mutual hostility and even war is a result of a different mindset, interpretation pattern point of view of what they think is the way to go, the best way, the solution to achieve their destination. So that the underlying conditions that declares the special attention it is necessary in every teaching the importance of togetherness, mutual understanding, mengedepankan tolerance, and the principle that religion handed down to this earth to bring peace and tranquility.
VARIASI INTRASPESIES LACTOBACILLUS PLANTARUM (ORLA-JENSEN) BERGEY ET AL. ASAL SAYUR ASIN BERDASARKAN ANALISIS MOLEKULER ., Sulistiani; ., Abinawanto; Sukara, Endang; Dinoto, Achmad; Mangunwardoyo, Wibowo
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2162

Abstract

The current study is the first report on intraspecies analysis of L. plantarum from sayur asin in Indonesia using molecular approach. Three molecular techniques, i.e., restriction fragment length polymorphism (RFLP) 16S-23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and enterobacterial repetitive intergenic consensus (ERIC-PCR) were used to determine the intraspecies diversity of L. plantarum responsible for spontaneous fermentation in sayur asin. These methods were aimed to discriminate 46 isolates of L. plantarum isolated from sayur asin, including the type strain. PCR amplification of the 16S-23S rDNA ISR revealed two-bands profile of 800 and 600 bp specific to lactobacilli. RAPD-PCR and ERIC-PCR were very valuable in discriminating genetic polymorphism among L. plantarum isolates by producing bands ranged from 4-10 bands (360-2620 bp) and 6-12 bands (160-2900 bp), respectively. Dendograms generated from UPGMA cluster analysis based on RAPD-PCR and ERIC-PCR data showed that all isolates were grouped into three major clusters with 74% and 68.6% genetic similarity thresholds, respectively.The study indicated that strains belong to L. plantarum isolated from sayur asin were divided into three genotypic groups. Keywords: ERIC-PCR, Intraspecies, Lactobacillus plantarum, RAPD-PCR, RFLP 16S-23S rDNA ISR 
OPTIMASI ENZIM ?-AMILASE DARI BACILLUS AMYLOLIQUEFACIENS O1 YANG DIINDUKSI SUBSTRAT DEDAK PADI DAN KARBOKSIMETILSELULOSA Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2200

Abstract

 ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media.  The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth. Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcellulose 
Co-Authors Abinawanto Abinawanto Achmad Dinoto Endang Sukara Maman Rahmansyah WIBOWO MANGUNWARDOYO Yati Sudaryati Soeka