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All Journal Buletin Peternakan JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA
Maman Rahmansyah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary Other

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Journal : JURNAL BIOLOGI INDONESIA

 1   2 
Pseudozyma aphidis as inoculant for local chicken Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.679 KB) | DOI: 10.14203/jbi.v12i1.2327

Abstract

SHORT COMMUNICATION
Pertumbuhan Bakteri Hasil Isolasi dari Tanah Perkebunan yang Tumbuh pada Media Mengandung Pestisida Propoksur dan Karbaril Imamuddin, Hartati; Laili, Nur; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.636 KB) | DOI: 10.14203/jbi.v8i2.3054

Abstract

Growth Capacity of Soil Bacteria Isolates Obtained from Estate Area from Media Containing Propoxur and Carbaryl. Isolation actions have been carried out with three soil samples derived from soil estate of pine apple, sugar cane, and banana in Lampung area, Southern Sumatra. The isolates were grown in the selected media containing carbaryl insecticide, and herbicide of diuron and bromocyl. The highest and vigorous growth isolates have gathered from threedifferent soil samples, and subsequently named GGPC, GM, and NTF isolates. Furthermore, those isolates were studied through the growth rate in the media containing propoxur and carbaryl in the nutrient rich liquid (NB) and compared to limited mineral (MM). All of the isolates grew faster against pesticides in the liquid medium of NB compared to MM. High correlated value among parameters nurtured as due to bacterial population and those processes were not affected by media composition. The GM isolate showed similar pattern during propoxur decrease along with incubation even though the rate of pesticide elimination from the media was higher in the rich nutrient liquid media (NB) compared to MM.Keywords: microbe isolate, pesticide, propoxur, carbaryl
Pengaruh Pemberian Kompos dan Pupuk Hayati terhadap Aktivitas Fosfatase dan Urease pada Tanah yang Ditanami Brokoli (Brassica oleracea L.) Rahmansyah, Maman; Antonius, Sarjiya
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2163

Abstract

Organic farming basically work on improving organic soil substrate which give impact to plant growth promoting bacteria. On the other hand, the use of agrochemicals leave chemical residues in the soil. In an effort to get the quality of organic matter and selected microbes as a biofertilizer material, this research had been done. Half ripe composting (HRC) process of organic substrate was utilized to enrich soil containing agrochemical residue, while ripe compost (RC) substance amended to soil free residue. That compost was used in broccoli cultivation. Microbial degrading pesticide was added to compost as biofertilizer formula. Even though plant biomass and the yield of flower was unclearly affected, but moreover the HRC application as due to “semi-on-site-composting” practices in soil containing agrochemical residue would cut off compost processing time. Soil microbial activity measured through soil respiration, urease and phosphomonoesterase indicated that the value was lower in samples collected from the soil free residue compared with soils containing residues. Utilization of chicken manure resulted in the availability of high nitrogen compounds and causing imbalance absorption by plants, compared with the use of HRC and RC in this study. Keywords: soil respiration, phosphatase, urease, Brassica oleracea L. 
Optimasi Enzim ?-Amilase dari Bacillus amyloliquefaciens O1 yang Diinduksi Substrat Dedak Padi dan Karboksimetilselulosa Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1523.768 KB) | DOI: 10.14203/jbi.v11i2.2200

Abstract

 ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media.  The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth. Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcellulose 
Production of Acid Phosphatase in Bacillus sp. Isolated from Forest Soil of Gunung Salak National Park Rahmansyah, Maman; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3140

Abstract

ABSTRAKProduktivitas Fosfatase Asam pada Bacillus sp. yang Diisolasi dari Tanah Hutan TamanNasional Gunung Salak. Pada pengamatan ini dilakukan karakterisrik bakteri pelarut fosfatyang diisolasi dari tanah hutan Taman Nasional Gunung Salak. Sebanyak 21 koloni hasil isolasidiuji terhadap produktivitas enzim fosfatase berdasar pelarutan media mengandung fosfat.Isolat yang terkuat melarutkan fosfat diidentifikasi sebagai Bacillus sp. Pada pengamatanlanjutan terhadap strain teruji dilakukan penumbuhan pada media cair selama 90 jam inkubasi,dan hasilnya ternyata mampu melarutkan fosfat inorganik (Pi) dari sumber trikalsium fosfat(Ca-Pi) dan alumunium fosfat (Al-Pi) masing-masing pada kisaran 1,2 sampai 152 dan 0.8 sampai25 mg.L-1; dan menunjukkan aktifitas enzim fosfomonoesterase antara 0.2 sampai 1.01 unitpada media yang mengandung larutan para-nitrophenylphosphate sebagai media fosfatorganik (Po) artifisial. Konsumsi glukosa pada media yang diukur selama pertumbuhan sejalanpula dengan produk ortofosfat sebagai akibat adanya aktifitas enzim fosfatase. Peningkatanfosfatase juga sejalan dengan bertambahnya biomassa sel bakteri dan penambahan produkasam glukonat. Penurunan pH dari 7 menjadi 5 diakibatkan peningkatan produk asam glukonatdi dalam media tumbuh. Bakteri pelarut fosfat yang berasal dari tanah hutan Taman NasionalGunung Salak dapat memproduksi fosfatase asam untuk memineralisasi sumber-sumber fosfatmenjadi sumber nutrisi yang siap digunakan oleh akar tumbuhan, dan itu merupakan prediksikuat untuk menjadikan isolat bakteri pelarut fosfat sebagai sumber bahan pupuk hayati.Kata kunci: Bacillus sp., tanah hutan, fosfatase asam, Ca-Pi, Al-Pi.
Pengaruh Inokulasi Bakteri Terhadap Pertumbuhan Awal Jarak Pagar (Jatropha curcas L.) Widawati, Sri; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3175

Abstract

ABSTRACTBacterial inoculants affect the early growth of Jatropha (Jatropha curcas L). Genera ofAzotobacter, Bacillus, Chromobacterium, Citrobacter, Nitrosomonas, Rhizobium, andSpaerotillus natans were soil bacterial isolates. The soil was collected from numerous placesaround Pontianak, West Kalimantan. Those isolates were used as inoculants, and formulatedto single and mixed bacterial inoculants, then used to stimulate the early growth of jatrophaseedling in 15 weeks at greenhouse condition. Bacterial inoculations caused better growthperformance compared to its control as pure soil garden medium without inoculations, andneither to bare soil dresses with compost. In the presence of inoculants, plant height wasaccelerated quickly while other inoculants affected to stalk diameter development. Daily growthperformance of jatropha peaked in 8 and 11 weeks after inoculation of Citrobacter andNitrosomonas bacterial component were used as single inoculant, respectively. The increasingof shoot biomass accumulation was three times as caused by single inoculants (Bacillus sp),and the highest one up to four times of biomass weight caused by a mixture inoculants asconsortium of Azotobacter, Bacillus, and Nitrosomonas spp. That selective inoculant hasopportunity to be used for jatropha farming, and this basic study is meaningful to jatropacultivation for standing to bio-fuel resources.Keywords: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.Kata kunci: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.
Ragam Aktivitas Urease dan Fosfomonoesterase serta Perannya dalam Ketersediaan Nutrisi N dan P pada Tanah Kebun Biologi Wamena Rahmansyah, Maman; Latupapua, H.J.D.; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.513 KB) | DOI: 10.14203/jbi.v3i4.3325

Abstract

ABSTRACTDiscrepancy of urease and phosphomonoesterase activities and its role in establishing Nand P nutrition in soil collected from Wamena Biological Research Station. Microbialactivities in soil lead to know for establishing soil nutrient status. Accordingly, soil collectedfrom Biological Research Station in Wamena then sent to the laboratory and determined on itsenzymatic activities and the physicochemical, as well. In this work, the enzymatic activities ofurease and phosphomonoesterase were examined in relation with soil microbial respiration, inorder to understand the mineralization of nitrogenous and phosphorus compound in soil. Soilrespiration rate (2.43-3.21 mg C02 g-dm12hour) designated variation in each sample, as wellas urease (8.6-23.5 unit/g soil) and phosphomonoesterase (5.5-7.9 unit/g soil) activities.Phosphomonoesterase activity showed strong correlation with respiration rate within soil; andreveal to the configuration of the bioactivities and physicochemical soil figures concluded thatthe B sample has the poor fertility. The phenomenon of data fulfill that bioactivities hadcorrelation with the physicochemical compound in the soil.Keywords: respiration, urease, phosphomonoesterase, Wamena Biological Research Station.
PSEUDOZYMA APHIDIS AS INOCULANT FOR LOCAL CHICKEN Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2327

Abstract

SHORT COMMUNICATION
RAGAM AKTIVITAS UREASE DAN FOSFOMONOESTERASE SERTA PERANNYA DALAM KETERSEDIAAN NUTRISI N DAN P PADA TANAH KEBUN BIOLOGI WAMENA Rahmansyah, Maman; Latupapua, H.J.D.; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3325

Abstract

ABSTRACTDiscrepancy of urease and phosphomonoesterase activities and its role in establishing Nand P nutrition in soil collected from Wamena Biological Research Station. Microbialactivities in soil lead to know for establishing soil nutrient status. Accordingly, soil collectedfrom Biological Research Station in Wamena then sent to the laboratory and determined on itsenzymatic activities and the physicochemical, as well. In this work, the enzymatic activities ofurease and phosphomonoesterase were examined in relation with soil microbial respiration, inorder to understand the mineralization of nitrogenous and phosphorus compound in soil. Soilrespiration rate (2.43-3.21 mg C02 g-'dm12hour) designated variation in each sample, as wellas urease (8.6-23.5 unit/g soil) and phosphomonoesterase (5.5-7.9 unit/g soil) activities.Phosphomonoesterase activity showed strong correlation with respiration rate within soil; andreveal to the configuration of the bioactivities and physicochemical soil figures concluded thatthe B sample has the poor fertility. The phenomenon of data fulfill that bioactivities hadcorrelation with the physicochemical compound in the soil.Keywords: respiration, urease, phosphomonoesterase, Wamena Biological Research Station.
PENGARUH PEMBERIAN KOMPOS DAN PUPUK HAYATI TERHADAP AKTIVITAS FOSFATASE DAN UREASE PADA TANAH YANG DITANAMI BROKOLI (BRASSICA OLERACEA L.) Rahmansyah, Maman; Antonius, Sarjiya
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2163

Abstract

Organic farming basically work on improving organic soil substrate which give impact to plant growth promoting bacteria. On the other hand, the use of agrochemicals leave chemical residues in the soil. In an effort to get the quality of organic matter and selected microbes as a biofertilizer material, this research had been done. Half ripe composting (HRC) process of organic substrate was utilized to enrich soil containing agrochemical residue, while ripe compost (RC) substance amended to soil free residue. That compost was used in broccoli cultivation. Microbial degrading pesticide was added to compost as biofertilizer formula. Even though plant biomass and the yield of flower was unclearly affected, but moreover the HRC application as due to â??semi-on-site-compostingâ? practices in soil containing agrochemical residue would cut off compost processing time. Soil microbial activity measured through soil respiration, urease and phosphomonoesterase indicated that the value was lower in samples collected from the soil free residue compared with soils containing residues. Utilization of chicken manure resulted in the availability of high nitrogen compounds and causing imbalance absorption by plants, compared with the use of HRC and RC in this study. Keywords: soil respiration, phosphatase, urease, Brassica oleracea L. 
Co-Authors Antonius, S Arwan Sugiharto Atit Kanti Dwi Agustiyani Hartati Imamuddin Hartati Imanuddin I Made Sudiana I Made Sudiana Imanuddin, Hartati LATUPAPUA, H.J.D. Latupapua, H.J.D. Martono, Budhi Muslichah, Dwi Agustiyani NUNIK SULISTINAH Nur Laili Rahayu, Ritai Dwi Ritai Dwi Rahayu Sarjiya Antonius Sri Widawati Suliasih Suliasih Sulistiani ., Sulistiani Yati Sudaryati Soeka