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Reni Nurjasmi

Unknown Affiliation

Author-ID : 359428

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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Pengaruh Jenis Ikan dan Media Tanam terhadap Pertumbuhan dan Hasil Tanaman Sayuran Buah pada Sistem Akuaponik Suryani Suryani; Reni Nurjasmi
JURNAL PERTANIAN Vol 7, No 2 (2016): Jurnal Ilmiah Respati
Publisher : Universitas Respati Indonesia

Dalam sistem budidaya terpadu sayuran dan ikan, pemilihan komoditas memegang peranan penting dalam merencanakan dan mendapatkan hasil sesuai dengan apa yang diinginkan. Komoditas tanaman yang diusahakan dalam sistem tersebut mempunyai nilai ekonomis dan dibutuhkan oleh masyarakat. Akuaponik tidak hanya baik untuk sayuran hijau namun juga dapat diaplikasikan menggunakan beberapa varietas sayuran buah seperti terung, cabai, tomat, wortel, dan bit. Selain komoditas ikan, media tanam merupakan faktor penentu penting dalam mencapai keberhasilan penanaman menggunakan sistem akuaponik. Tujuan penelitian adalah mengetahui pengaruh interaksi jenis ikan dan media tanam terhadap pertumbuhan dan hasil tanaman sayuran buah pada sistem akuaponik serta interaksi jenis ikan dan media tanam yang terbaik. Penelitian menggunakan Rancangan Acak Kelompok Faktorial (RAKF) yang terdiri atas dua faktor dengan tiga ulangan. Faktor pertama adalah jenis ikan (gurame dan nila) dan Faktor kedua adalah media tanam (batu apung, kerikil dan zeolit). Perlakuan diuji menggunakan tiga jenis tanaman sayuran buah yaitu tomat, terung ungu, dan cabai rawit. Variabel pengamatan meliputi sifat kimia kotoran ikan, tinggi tanaman, jumlah daun, berat segar tanaman, berat kering tanaman, dan bobot buah. Hasil penelitian menunjukkan perlakuan media tanam berpengaruh nyata terhadap berat kering tanaman tomat dan berat segar tanaman terung ungu. Media tanam kerikil menghasilkan berat kering tanaman tomat tertinggi (241,01 gram) namun tidak berbeda dengan media tanam zeolit (121,75 gram) serta menghasilkan berat segar tanaman terung ungu tertinggi (8,34 gram) namun tidak berbeda dengan media tanam batu apung (6,22 gram). Kesimpulannya Perlakuan media tanam berpengaruh nyata terhadap berat kering tanaman tomat dan berat segar tanaman terung ungu.

Diversity of Actinomycetes at Several Forest Types in Wanagama I Yogyakarta and Their Potency as a Producer of Antifungal Compound Reni Nurjasmi; Jaka Widada; N. Ngadiman
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

Actinomycetes are bacterial groups that produce many secondary metabolites, which different biological activities, such as antifungi, antibacteria, antivirus, antitumor, etc. Actinomycetes are widely distributed in soil and their diversity is influenced by type of forest. The aim of this study is to investigate diversity of actinomycetes in several forest types of Wanagama I forest in Yogyakarta and their potency as a producer of antifungal compound. Soil samples under the forest of Tectona grandis, Swietenia macrophylla King, Bamboosa vulgaris, Melaleuca leucadendron, and Gliricidia maculata were used as sources of soil bacteria. Bacteria and actinomycetes communities were analyzed through culture-independent approach by RISA and nested-PCR RISA using actinomycetes spesific primer (F243), respectively. Through culture-dependent approach, isolated actinomycetes diversity were analyzed by identification of morphology (colony and cell), genetic (BOX element by rep-PCR), and secondary metabolites (thin layer chromatography). In addition, isolates were assayed for their antifungal activity against Saccharomyces cerevisae, Candida albicans, Fusarium oxysporum and Aspergillus flavus. The presence of Polyketide Synthase-I (PKS-I) and NonRibosomal Peptide Synthetase (NRPS) genes were amplified by PCR to study their correlation with antifungal activity of the actinomycete isolates. The results showed that types of forest influence diversity of rhizobacteria especially actinomycetes. According to culture-independent approach, relatively, com-</div><div>munity of rhizobacteria from the highest were soil under the forest of B. vulgaris, G. maculata, T. grandis, S.macrophylla King, and M. leucadendron, respectively. Meanwhile, community of actinomycetes from the highest were soil under the forest of G. maculata, B. vulgaris, M. leucadendron, S. macrophylla King, and T. grandis, respec- tively. Fourty-three morphologically different isolates were found by using culture-dependent approach consisting of 17 isolates were found in soil under the forest of M. leucadedron, each of 9 isolates in G. maculata and T. grandis, 6 isolates in S. macrophylla King. and 2 isolates in B. vulgaris. More diversity of secondary metabolites were observed in soil actinomycetes under the forest of M. leucadendron. Of the 43 isolates, 100% were active against S.cerevisae, 37.20% against C. albicans, 95.30% against F. oxysporum, and 83.70% against A. flavus. Antifungal activity of actinomycete isolates did not always have correlation with the presence of PKS-I and NRPS.

An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes A. Alimuddin; Widya Asmara; Jaka Widada; M. Mustofa; Reni Nurjasmi
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

Actinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and many of them are described as being produced by polyketide synthetases (pks). We present strain of Actinomycetes producing anticandida isolated from rhizosphere plant for amplification of Pks-I genes. The isolate was obtained from Wanagama I Forest UGM Yogyakarta. Gene of seven isolates, from total of 173 isolates, were amplified using degenerate primer to detect the presence of pks genes. One strain that is named Streptomyces sp. GMR-22 was partialy identified as anticandida producing actinomycete. The strain shown the strongest activity against Candida albicans. Based on bioautography assay, one spot active with Rf 0.57 was appeared as bright yellow by cerrium sulphate but it was and not visible on UV254 and 366 lights. Key words : pks genes, anticandida, Streptomyces sp GMR-22, rep-PCR, cajuput rhizosphere

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