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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya
Sumarno Retoprawiro
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Agriculture, Biological Sciences & Forestry Environmental Science Medicine & Pharmacology

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Hubungan antara Kadar Tumor Necrosis Factor-Alpha (TNF-α) Plasma dengan Kejang Demam Sederhana pada Anak Nurindah, Dewi; Muid, Masdar; Retoprawiro, Sumarno
Jurnal Kedokteran Brawijaya Vol 28, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.06 KB) | DOI: 10.21776/ub.jkb.2014.028.02.10

Abstract

Kejang demam adalah penyebab kejang paling umum pada anak dan sering menjadi penyebab rawat inap di rumah sakit secara darurat. Studi pendahuluan pada anak menunjukkan bahwa jaringan sitokin diaktifkan dan mungkin berperan dalam patogenesis kejang demam namun, signifikansi klinis yang tepat masih belum jelas. Penelitian ini bertujuan untuk membuktikan hubungan antara kadar TNF-α plasma dengan kejang demam sederhana. Penelitian cross sectional dilakukan pada Maret-April 2014 di Departemen Ilmu Kesehatan Anak Rumah Sakit Umum dr. Saiful Anwar Malang. Terdapat 38 subjek yang memenuhi kriteria inklusi, 19 pasien kejang demam sederhana dan 19 pasien demam tanpa kejang (usia 6 bulan-5 tahun). Kadar TNF-α plasma diperiksa dengan ELISA. Analisis Independent t test menunjukkan tidak terdapat perbedaan bermakna karakteristik subjek, suhu rektal dan kadar lekosit. Analisis Mann-Whitney menunjukkan tidak terdapat perbedaan bermakna karakteristik subjek  jenis kelamin dan kadar CRP. Kejang demam lebih banyak ditemukan pada usia yang lebih muda dibandingkan demam tanpa kejang. Hasil Independent t test juga menunjukkan terdapat perbedaan bermakna antara kadar TNF-α plasma kelompok kejang demam sederhana dan kelompok demam tanpa kejang (p=0,002). Hasil uji Spearman menunjukkan terdapat korelasi negatif sedang yang bermakna antara kadar TNF-α plasma kelompok kejang demam sederhana dan kelompok demam tanpa kejang (r=-0,533; p=0,001). Dapat disimpulkan bahwa terdapat hubungan antara kadar TNF-α plasma dengan terjadinya kejang demam sederhana.Kata Kunci: Anak, kejang demam sederhana, TNF-α plasma
Inhibition of Klebsiella pneumoniae adhesion in mice enterocytes by antibodies of hemagglutinin pili protein with MW 12.8 kDa of Klebsiella pneumoniae Agustina, Dini; Retoprawiro, Sumarno; AS, Noorhamdani
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Klebsiella pneumoniae as one of the most common cause of VAP is also the second most common cause of community- and hospital-acquired gram negative bloodstream infection. The process of infection caused by direct contact with infectious agents begins with the host cell adhesion process either by pili or by afimbria adhesin (AFA). There were no reported researches on the hemagglutinin pili protein of K.pneumoniae as adhesion factors.This study was conducted in order to determine the hemagglutinin pili protein of K.pneumoniae, polyclonal antibody produced from pili protein immunization, and its ability to inhibit K.pneumoniae adhesion in mice enterocytes. Adhesion inhibition test used HA antibody with the implementation of dose dilutions of 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200 and 0 (control). While immunocytochemistry test used HA pili protein with the implementation of dose dilutions of 1/10000, 1/20000, 1/40000, 1/80000, 1/160000, 1/320000 and 0 (control). Hemagglutinin pili protein found in K.pneumoniae had MW 12.8 kDa. Pearson correlation analysis of adhesion test showed there were significant correlation between antibody dilution titer with bacterial adhesion (p = 0.032, R = -0.797). Furthermore, Anova analysis of IT showed that there were significant differences between the various dilution titer with antigen-antibody reaction (p=0.000). Antibody of hemagglutinin pili protein with MW 12.8 kDa of K.pneumoniae can inhibit the adhesion of K.pneumoniae to the enterocytes of mice.
Inhibition of Klebsiella pneumoniae adhesion in mice enterocytes by antibodies of hemagglutinin pili protein with MW 12.8 kDa of Klebsiella pneumoniae Dini Agustina; Sumarno Retoprawiro; Noorhamdani AS
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Klebsiella pneumoniae as one of the most common cause of VAP is also the second most common cause of community- and hospital-acquired gram negative bloodstream infection. The process of infection caused by direct contact with infectious agents begins with the host cell adhesion process either by pili or by afimbria adhesin (AFA). There were no reported researches on the hemagglutinin pili protein of K.pneumoniae as adhesion factors.This study was conducted in order to determine the hemagglutinin pili protein of K.pneumoniae, polyclonal antibody produced from pili protein immunization, and its ability to inhibit K.pneumoniae adhesion in mice enterocytes. Adhesion inhibition test used HA antibody with the implementation of dose dilutions of 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200 and 0 (control). While immunocytochemistry test used HA pili protein with the implementation of dose dilutions of 1/10000, 1/20000, 1/40000, 1/80000, 1/160000, 1/320000 and 0 (control). Hemagglutinin pili protein found in K.pneumoniae had MW 12.8 kDa. Pearson correlation analysis of adhesion test showed there were significant correlation between antibody dilution titer with bacterial adhesion (p = 0.032, R = -0.797). Furthermore, Anova analysis of IT showed that there were significant differences between the various dilution titer with antigen-antibody reaction (p=0.000). Antibody of hemagglutinin pili protein with MW 12.8 kDa of K.pneumoniae can inhibit the adhesion of K.pneumoniae to the enterocytes of mice.
Co-Authors Dewi Nurindah Dini Agustina Masdar Muid Noorhamdani AS Noorhamdani AS