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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Clinical Microbiology and Infectious Diseases
Noorhamdani AS
Noorhamdani, Department of Microbiology, Faculty of Medicine, University of Brawijaya Jl. Veteran Malang 65145, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Environmental Science Immunology & microbiology

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Inhibition of Klebsiella pneumoniae adhesion in mice enterocytes by antibodies of hemagglutinin pili protein with MW 12.8 kDa of Klebsiella pneumoniae Dini Agustina; Sumarno Retoprawiro; Noorhamdani AS
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Klebsiella pneumoniae as one of the most common cause of VAP is also the second most common cause of community- and hospital-acquired gram negative bloodstream infection. The process of infection caused by direct contact with infectious agents begins with the host cell adhesion process either by pili or by afimbria adhesin (AFA). There were no reported researches on the hemagglutinin pili protein of K.pneumoniae as adhesion factors.This study was conducted in order to determine the hemagglutinin pili protein of K.pneumoniae, polyclonal antibody produced from pili protein immunization, and its ability to inhibit K.pneumoniae adhesion in mice enterocytes. Adhesion inhibition test used HA antibody with the implementation of dose dilutions of 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200 and 0 (control). While immunocytochemistry test used HA pili protein with the implementation of dose dilutions of 1/10000, 1/20000, 1/40000, 1/80000, 1/160000, 1/320000 and 0 (control). Hemagglutinin pili protein found in K.pneumoniae had MW 12.8 kDa. Pearson correlation analysis of adhesion test showed there were significant correlation between antibody dilution titer with bacterial adhesion (p = 0.032, R = -0.797). Furthermore, Anova analysis of IT showed that there were significant differences between the various dilution titer with antigen-antibody reaction (p=0.000). Antibody of hemagglutinin pili protein with MW 12.8 kDa of K.pneumoniae can inhibit the adhesion of K.pneumoniae to the enterocytes of mice.
The correlation between Candida colonization index and risk factors of invasive candidiasis among patient in intensive care unit Tiyakusuma, Evira; Noorhamdani AS; Ruliatna, Etty Fitria
Journal of Clinical Microbiology and Infectious Diseases Vol. 4 No. 1 (2024): Available online: June 2024
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v4i1.41

Abstract

Background: Invasive candidiasis is frequently found among patients hospitalized in intensive care units (ICU) due to immunocompromised state and performed the invasive procedure. To simplify invasive candidiasis screening, the Candida colonization index (CCI) was performed on the patients. But the implication of the CCI towards the risk factors of invasive candidiasis has never been evaluated before. Hence this study aims to evaluate the correlation between the CCI and invasive candidiasis risk factors and antifungal therapy. Methods: During this study, CCI was collected from routine clinical culture results of the ICU patients while information regarding risk factors was obtained from routine follow-ups conducted on patients in the ICU. This study showed a significant correlation between the CCI, and several risk factors of invasive candidiasis, such as sequential organ failure assessment (SOFA) score, number of invasive medical instruments, and Candida score. Results: These results support the hypothesis that proposes a certain correlation between CCI and risk factors of invasive candidiasis. There is also a significant correlation between antifungal therapy and the prevalence of Candida non-albicans colonization. Conclusion: This study also supports the hypothesis that proposes shifting in the distribution of Candida species causing invasive candidiasis from Candida albicans to Candida non-albicans due to misappropriate use of antifungal therapy.
Co-Authors Dini Agustina Ruliatna, Etty Fitria Sumarno Retoprawiro Tiyakusuma, Evira