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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Molekul: Jurnal Ilmiah Kimia
Juwarno Juwarno
Fakultas Biologi Unsoed
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science

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Molecular Profiles of Five Salinity-Resistant Soybean {glycine max (L.) Merr.} Cultivars Juwarno Juwarno; Hartanto Nugroho; Triani Hardiyati; Alice Yuniaty
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.513 KB) | DOI: 10.20884/1.jm.2020.15.3.628

Abstract

In this study, the molecular profiles of five soybean cultivars (Burangrang, Gema, Grobogan, Panderman, and Sinabung) exhibiting salinity resistance were elucidated. The DNA profiles of the five cultivars were found to differ based on simple sequence repeat (SSR), insertion-deletion polymorphism (InDel)-QS080465, and sequence characterized amplified region (SCAR)-QS08064 markers. Three distinct SSR profiles¾Satt-243, Satt-294, and Satt-308¾and the SCAR-QS08064 marker were only observed in the Grobogan cultivar, whereas the InDel-QS080465 marker was only successfully amplified from the Burangrang, Gema, and Grobogan cultivars. The results indicate that the Grobogan cultivar is the most tolerant soybean cultivar, followed by the Burangrang and Gema cultivars. Results were consistent with those from genetic similarity analysis, which showed that Grobogan is genetically more similar to Burangrang and Gema compared to Sinabung and Panderman. In conclusion, the five soybean cultivars have different molecular profiles that are related to their resistance to salinity. SSR markers, InDel QS080465-152, and SCAR QS08064-383 are molecular markers specific to salinity-resistant cultivars.
Karakter Antomi Daun Tumbuhan Mangrove Akibat Pencemaran di Hutan Mangrove Kabupaten Cilacap Siti Samiyarsih; Tata Brata Suparjana; Juwarno Juwarno
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 1 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.1.288

Abstract

This research was aimed to observe the anatomical character of mangrove plants leave and examine the differences from leaves anatomical character of mangroves in Donan river and Tritih forest in Cilacap regency as an self-adaptation indicator. The method which is used was survey method by collecting leaves sample of each mangrove plants. The sample was preparated using embedding method, and stained with safranin 1% disolved in alcohol 70%. The parameter of variable which is observed in leaves anatomical character are thickness of leaves,  leaves mesophyll thickness, cuticle thickness, stomata length size, and amount of stomata and trichome per leaves unit area. The data were analyzed with t-test for examining anatomical character difference. The results showed that four usual types of mangrove plants such as Achantus ilicifolius, Rhizospora mucronata, Aegiceros corniculatum, and Bruguiera gymnorrhiza have similar leaves anatomical structure. The anatomical character from polluted habitat were different from unpolluted habitat. This differences was showed in leaves mesophyll thickness, cuticle thickness, stomata length size, and amount of stomata and trichome per mm2 leaves unit area. 
The Effects of Nitrogen Fertilizer Dosages on Anatomical Characters of Ipomoea batatas L. Leaf Juwarno Juwarno; Sumarsono Sumarsono; Siti Samiyarsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 1 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.1.915

Abstract

The research was conducted in order to understand the effects of various nitrogen dosages on the anatomical characters of Ipomoea batatas leaf, and to find out the most influential dosage on the number and size of leaf anatomical characters. The characters observed consisted of thickness of cuticle, leaf, and palisade, as well as stoma size, and their density within mm2 of leaf surface. The method applied was experiments arranged in Completely Randomized Design. The results showed that all levels of nitrogen dosage affected the anatomical characters of sweet potato leaf while the most influential dosage was 200 kg urea per ha.
Co-Authors Alice Yuniaty Hartanto Nugroho Siti Samiyarsih Sumarsono Sumarsono Tata Brata Suparjana Triani Hardiyati