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STUDI KEANEKARAGAMAN GENETIK TANAMAN KECIPIR (PSOPHOCARPUS TETRAGONOLOBUS (L.) DC): UPAYA MENUJU LABORATORIUM GENETIKA SEBAGAI PUSAT KAJIAN KETAHANAN PANGAN DAN BIOENERGI Amurwanto, Adi; Yuniaty, Alice; Susanto, Agus Hery; Sasongko, Nurtjahjo Dwi; Aziz, Saefuddin
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Kecipir mempunyai potensi untuk dimanfaatkan sebagai bahan pangan segar maupun olahan bernilai gizi tinggi dan sebagai bahan dasar dalam industri biodiesel. Pemanfaatan kecipir yang belum optimal mendorong dilakukannya penelitian tentang keanekaragaman genetik tanaman kecipir sebagai langkah awal untuk mengembangkan potensi tanaman ini. Penelitian ini bertujuan untuk memperoleh data mengenai keanekaragaman genetik pada level molekuler kecipir polong panjang dan polong pendek dengan teknik RAPD. Dari 32 primer yang diskrining, 20 dipilih untuk analisis menggunakan Program GenAlEx 6.1. Hasilnya 156 buah fragmen dengan 140 diantaranya menunjukkan polimorfisme sehingga didapatkan polimorfisme total sebesar 89,74%. Polimorfisme dalam populasi kecipir polong pendek bernilai 64.10% dan polimorfisme dalam populasi kecipir polong panjang sebesar 51.28%. Kemiripan genetik Nei (bias) antara kecipir polong panjang dan kecipir polong pendek adalah 0,805 sementara jarak genetik antara keduanya adalah 0,218. Adapun kemiripan genetik tidak bias antara kedua populasi adalah 0,873, sementara jarak genetik tidak bias sebesar 0,136.

PENGARUH FOTOPERIOD DAN GIBERELIN TERHADAP PERTUMBUHAN DAN PEMBUNGAAN IN VITRO KRISAN (Chrysanthemum sp.) & Allice Yuniaty, Angga Adriana Imansyah & Sugiyono
AGROSCIENCE (AGSCI) Vol 6, No 2 (2016): December
Publisher : Fakultas Sains Terapan, Universitas Suryakancana Cianjur

Penelitian ini bertujuan untuk mempelajari pengaruh interaksi antara fotoperiode dengan giberelin (GA3) dan menentukan kombinasi terbaik antara fotoperiode dengan konsentrasi GA3 untuk pertumbuhan dan pembungaan krisan (Cryhsanthemum sp.) secara in vitro. Penelitian dilakukan secara ekperimental menggunakan metode Rancangan Acak Lengkap (RAL) dengan 2 faktor. Faktor pertama adalah fotoperiode yang terdiri dari empat taraf yaitu P1 (13,5 jam gelap dan 10,5 jam terang), P2 (15 jam gelap dan 9 jam terang), P3 (16,5 jam gelap dan 7,5 jam terang), dan P4 (18 jam gelap dan 6 jam terang), dan faktor kedua adalah konsentrasi GA3 yang terdiri dari empat taraf yaitu G1 (0 Î¼M GA3), G2 (1,5 Î¼M GA3), G3 (3 Î¼M GA3) dan G4 (4,5 Î¼M GA3). Secara total terdapat 16 kombinasi perlakuan dan diulang 3 kali. Data yang diperoleh dianalisis ragam atau analysis of variant (ANOVA) dengan menggunakan Instrumen SAS. Jika perlakuan menunjukkan perbedaan yang nyata, dilanjutkan dengan Duncan Multiple Range Test (DMRT) guna mengetahui perbedaan antar perlakuan. Hasil penelitian menunjukkan bahwa perlakuan fotoperiode dan GA3 tidak mampu menginduksi pembungaan krisan (Cryhsanthemum sp.) secara in vitro, akan tetapi fotoperiode secara tunggal yaitu P4 (18 jam gelap dan 6 jam terang) mempengaruhi tinggi tanaman, jumlah ruas, panjang ruas, jumlah daun, dan panjang akar.

Molecular Profiles of Five Salinity-Resistant Soybean {glycine max (L.) Merr.} Cultivars Juwarno Juwarno; Hartanto Nugroho; Triani Hardiyati; Alice Yuniaty
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

In this study, the molecular profiles of five soybean cultivars (Burangrang, Gema, Grobogan, Panderman, and Sinabung) exhibiting salinity resistance were elucidated. The DNA profiles of the five cultivars were found to differ based on simple sequence repeat (SSR), insertion-deletion polymorphism (InDel)-QS080465, and sequence characterized amplified region (SCAR)-QS08064 markers. Three distinct SSR profiles¾Satt-243, Satt-294, and Satt-308¾and the SCAR-QS08064 marker were only observed in the Grobogan cultivar, whereas the InDel-QS080465 marker was only successfully amplified from the Burangrang, Gema, and Grobogan cultivars. The results indicate that the Grobogan cultivar is the most tolerant soybean cultivar, followed by the Burangrang and Gema cultivars. Results were consistent with those from genetic similarity analysis, which showed that Grobogan is genetically more similar to Burangrang and Gema compared to Sinabung and Panderman. In conclusion, the five soybean cultivars have different molecular profiles that are related to their resistance to salinity. SSR markers, InDel QS080465-152, and SCAR QS08064-383 are molecular markers specific to salinity-resistant cultivars.

KEKERABATAN MOLEKULER DARI KULTIVAR BUNGA MATAHARI TEDDY BEAR, SKYSCRAPER, LEOM QUEEN DAN BUNGA MATAHARI MENGGUNAKAN MARKA RAPD Gabriella Ashari Tanudharma; Nurtjahjo Dwi Sasongko; Alice Yuniaty
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Helianthus or sunflower is a genus of plant comprising about 70 species. Common sunflower and other members of Helianthae are cultivated in temperate regions and some tropical regions as food crops for humans, cattle, poultry, and as ornamental plants. The common sunflower is valuable with respect of economic and ornamental point of view. There are many cultivars of sunflower including teddy bear, skyscraper, and lemon queen. Variation among these cultivars has been studied using molecular techniques and the result were used to develop the phylogeny among them. Random Amplified Polymorphic DNA (RAPD) is one of molecular techniques that were used for this purpose. The purpose of this study was to contruct the phylogeny of three sunflower cultivars and common sunflower based on RAPD markers. The RAPD primers used in this study were OPA-2, OPA-9, OPA-13, OPB-2, OPB-4, OPB5, OPB-7, and OPB-11. Data analysis based on molecular data showed that genetic relationship among Lemon Queen, Skyscraper, Teddy Bear and Common sunflower based on RAPD markers shows that the cultivars studied are grouped into three main groups, namely: Group I Lemon Queen and Skyscraper, Group II Teddy Bear, and Group III Common sunflower; the closest kinship is shown between Lemon Queen and Skyscraper.

Genetic Diversity Among Three Cultivars Of Peanut (Arachis hypogaea L.) Based On Rapd Markers Suryadi Suryadi; Alice Yuniaty; Agus Hery Susanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Peanut (Arachis hypogea) is a typical plant species of tropical regions that has high economic value. The plantation is widely spread over many areas and the production is being pushed to meet the increasing demand. Peanut breeding program is aimed to improve genetic quality, mainly with resepct of production and thus information on genetic diversity is necessary as a basis for consideration in breeding, management and sustainable utilization. One approach to analyse genetic diversity of peanut is by using molecular markers. Random Amplified Polymorphic DNA (RAPD) is a widely used molecular marker for genetic diversity analysis. Therefore, the aim of this study was to assess genetic diversity of peanut cultivars, i.e. Jerapah, Kancil, and Hypoma 2, based on RAPD markers. The study was conducted in a survey method, in which three individuals of each cultivar were analyzed using PCR-RAPD technique employing twelve primers, i.e. OPA-1, OPA-2, OPA-9, OPA-13, OPB-2, OPB-3, OPB-4, OPB-5, OPB-7, OPB-11, OPB-12 and OPJ-07. Data analysis based on morphological data is also included. Molecular analysis revealed that only 7.55% polymorphic band was obtained, while most of the bands were monomorphic, indicating very low variation among the cultivars. The phenogram that constructed based on literature showed that Kancil was closer to Jerapah cultivar, while RAPD-based dendogram showed that Hypoma 2 was closer to Kancil cultivar.

ANALISIS RAPD KECIPIR POLONG PANJANG Psophocarpus tetragonolobus (L.) DC HASIL MUTASI IRADIASI SINAR GAMMA Arini Maesaroh; Adi Amurwanto; Alice Yuniaty
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Winged bean [Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some benefits and is very suitable to be cultivated in Indonesia. Study about diversity of winged bean is very important to support the future development of winged bean. Increased genetic diversity can be done through mutation. Gamma ray is often used for inducting mutations. One of way to observe genetic diversity resulted by molecular mutation is using RAPD method. The purpose of this study was to identify the genetic diversity of winged bean resulted by gamma ray irradiation. The method used in this study was an experiment by using RAPD technique. Samples used were plant leaves that had been induced by gamma ray with a wavelength of 20 Gy, 25 Gy, and control plants that were not induced by gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA 18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30% and 54,10%, respectively. The values of genetic variation based on the calculation of allele frequencies were 0,236; 0,202 and 0,194 for treatment of 20 Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic distance ranged from 0.08 to 0.32.

VARIASI SEKUENS DNA YANG DIAMPLIFIKASI MENGGUNAKAN PRIMER atpB-rbcL PADA BEBERAPA KULTIVAR KACANG TANAH Yani Yuliani; Alice Yuniaty; Agus Hery Susanto
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Peanut is one of food crops commonly consumed in Indonesia. This species comprises several cultivars such as Kancil, Bison, Jerapah, Talam, and Tuban, each of which has its individual advantages and disadvantages. The vast variation among peanut cultivars leads to the need of study on genetic diversity and relationship among them using particular molecular marker. This study aims to see whether variation on DNA sequences among some peanut cultivars amplified with atpB-rbcL primers exists or not and to know the relationship among the cultivars based on the amplicon sequences. The method involves some sequential steps, i.e. genomic DNA isolation using CTAB protocol, amplification of DNA sequence using atpB-rbcL primers and sequencing of the amplification products. Data on sequences were edited manually using Bioedit version 7.0.4.1. Sequence alignment was performed using ClustalW, which is also implemented in Bioedit version 7.0.4.1. Arlequin 2.0 was used to calculate nucleotide diversity p. Phylogenetic analysis was performed using Maximum Parsimony in MEGA 5.0. The results showed that considerably high variation in DNA sequences of some peanut cultivars amplified with atpB-rbcL primers are observed. On the other hands, very close genetic relationship among cultivars is found.

Profil RAPD Tanaman Kantung Semar Beberapa Koleksi Kebun Raya Baturraden Retno Mayangsari; Agus Hery Susanto; Alice Yuniaty
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.484

Telah dilakukan penelitian tentang profil RAPD beberapa tanaman kantung semar (Nepenthes spp.) koleksi Kebun Raya Baturraden. Tujuh individu yang dipelajari merupakan spesies semiendemik Gunung Slamet dan endemik Jawa, yaitu N. adrianii dan N. gymnampora. Metode yang digunakan adalah survei dengan teknik pengambilan sampel secara acak. DNA genomik sampel diekstraksi untuk kemudian digunakan sebagai templat dalam amplifikasi marka RAPD menggunakan teknik PCR dengan 10 primer acak, yaitu ES10G23, ES10A26, ES10C24, ES10G33, OPA-2, OPA-9, OPA-13, OPB-3, OPB-5, dan OPB-7. Hasil penelitian menunjukkan bahwa ke-10 primer tersebut menghasilkan 43 (67,2%) pita polimorfik dan 21 (32,8%) pita monomorfik dengan ukuran berkisar dari 130 hingga 1.500 bp. OPA-2 and OPB-3 menghasilkan polimorfisme tertinggi, sedangkan ES10G23 dan OPA-13 menghasilkan pita monomorfik terbanyak. Dendogram yang dikonstruksi menggunakan UPGMA dalam MEGA 6.06 memperlihatkan bahwa dua klaster terbentuk pada jarak genetik 18%. Kedua klaster tersebut cenderung dipisahkan oleh lokasi geografi.

Shoot Tip Culture: A Potential In Vitro Culture Multiplication of Screw Pines (Pandanus tectorius Park.) Ikhsan - Matondang; Sisunandar .; Alice Yuniaty; Triani Hardiyati; Agus Hery Susanto; Endang Srimurni Kusmintarsih
BERITA BIOLOGI Vol 21, No 2 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i2.4208

Pandanus tectorius Park., tree of life, which almost all of its parts are used by humans. Industrial fiber made from screw pine leaves has been successfully exported to several countries, while the fruit is consumed as an alternative staple food. Fruit is only produced by female plants, because screw pine is dioecious plant. Farmers obtain screw pine seedling using stem cuttings from female trees, but the number of seedling produced is limited and this technique can damage the plant. Alternative seedling production using seeds has constraints in the form of uncertainty over the sex of the seedling. One of the best ways to provide screw pine seedling is to use a shoot-tip culture technique. Shoot-tip culture is able to reproduce plants efficiently and quickly and does not damage the plant. Seedling will also have the same genetic traits as the selected plant. However, efforts to develop an efficient and fast screw pine shoot-tip culture protocol have not been carried out massively. The induction and shoot multiplication stages still had a low success rate. Another problem in seedling production through tissue culture is the certainty seedlings are true-to-type. This review article aims to reveal the potential of shoot-tip culture for in vitro screw pine seedling production and to test the genetic stability of the screw pine seedling.

KEKERABATAN MOLEKULER DARI KULTIVAR BUNGA MATAHARI TEDDY BEAR, SKYSCRAPER, LEOM QUEEN DAN BUNGA MATAHARI MENGGUNAKAN MARKA RAPD Tanudharma, Gabriella Ashari; Sasongko, Nurtjahjo Dwi; Yuniaty, Alice
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1762

Helianthus or sunflower is a genus of plant comprising about 70 species. Common sunflower and other members of Helianthae are cultivated in temperate regions and some tropical regions as food crops for humans, cattle, poultry, and as ornamental plants. The common sunflower is valuable with respect of economic and ornamental point of view. There are many cultivars of sunflower including teddy bear, skyscraper, and lemon queen. Variation among these cultivars has been studied using molecular techniques and the result were used to develop the phylogeny among them. Random Amplified Polymorphic DNA (RAPD) is one of molecular techniques that were used for this purpose. The purpose of this study was to contruct the phylogeny of three sunflower cultivars and common sunflower based on RAPD markers. The RAPD primers used in this study were OPA-2, OPA-9, OPA-13, OPB-2, OPB-4, OPB5, OPB-7, and OPB-11. Data analysis based on molecular data showed that genetic relationship among Lemon Queen, Skyscraper, Teddy Bear and Common sunflower based on RAPD markers shows that the cultivars studied are grouped into three main groups, namely: Group I Lemon Queen and Skyscraper, Group II Teddy Bear, and Group III Common sunflower; the closest kinship is shown between Lemon Queen and Skyscraper.

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