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All Journal Molekul: Jurnal Ilmiah Kimia Scripta Biologica BioEksakta : Jurnal Ilmiah Biologi Unsoed
Ratna Stia Dewi
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Veterinary

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DEKOLORISASI LIMBAH BATIK TULIS MENGGUNAKAN JAMUR INDIGENOUS HASIL ISOLASI PADA KONSENTRASI LIMBAH YANG BERBEDA Ratna Stia Dewi; Sri Lestari
Molekul Vol 5, No 2 (2010)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.596 KB) | DOI: 10.20884/1.jm.2010.5.2.79

Abstract

Azo as batik dyes are textile dyes which difficult to degradate. Fungus as bioremidiation organism are choosed to decolorize the dyes because its transformation ability, it can degradate toxic dyes component. The aim of research are to explore the fungus from Sokaraja-Banyumas batik industrial dyestuff, to know potential indigenous species wich can degradate it, to know dyestuff consentration which is degradated.Result of research showed that the isolation process of indigenous fungi from batik dyestuff in District Sokaraja Banyumas produce 4 isolates that have the potential dekolorization, they are 3 isolates of the genus Fusarium, and 1 isolate of the genus Aspergillus. That indigenous fungus can be used to decolorize dyestuff batik the decolorize percentage 69.346% -82.421%.
DEKOLORISASI LIMBAH BATIK MENGGUNAKAN LIMBAH MEDIUM TANAM Pleurotus ostreatus PADA WAKTU INKUBASI YANG BERBEDA Fitriya Yuni Wulandari; Nuniek Ina Ratnaningtyas; Ratna Stia Dewi
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.479 KB) | DOI: 10.20884/1.sb.2014.1.1.29

Abstract

The batik waste industry is one source of water pollution. It must undergo treatment before discharge into the environment. Batik waste treatment technologies can be done biologically, chemically, physically, or combination of three processes. This research applied a combination of biology and physic technologies, namely used spent mushroom of Pleurotus ostreatus as the decolorization agent. Mycelium of P. ostreatus was used as biological agent, and cellulose from spent mushroom was used as physical agent. The aims of this research were to determine the effect of spent mushroom with variation incubation time on batik waste decolorization, and to know the optimum incubation time with highest decolorization batik waste percentage using spent mushroom of P. ostreatus. This research used experimental method with completely randomized design (CRD). The data obtained were statistically analyzed using Varian Test (ANOVA) on confidence level of 95% and 99% and continued with Honest Significant Difference Test (BNJ) with 95% confidence level. The result showed that variation incubation time treatment of batik waste decolorization used spent mushroom of P. ostreatus provide a highly signification effect on batik waste decolorization. The optimum incubation time was 60 hour (LW5), with decolorization percentage was 85,64%.
Aktivitas Selulase dan Pola Pertumbuhan Miselium Aspergillus RB1 (Fungi Selulolitik Asal TPST Rempoah, Banyumas) Berdasarkan Variasi Waktu Inkubasi Arif Rahman Hikam; Ratna Stia Dewi; Aris Mumpuni; Dwiana Muflihah Yulianti; Adinda Eka Murti Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.18491

Abstract

Fungi selulolitik merupakan salah satu mikroorganisme utama yang berperan dalam produksi enzim selulase karena kemampuannya mensekresikan enzim ekstraseluler dalam jumlah tinggi. Aspergillus RB1 merupakan isolat fungi selulolitik yang diperoleh dari Tempat Pengolahan Sampah Terpadu (TPST) Rempoah, Banyumas, dan berpotensi sebagai penghasil enzim selulase. Penelitian ini bertujuan untuk mengkaji aktivitas enzim selulase serta pola pertumbuhan miselium Aspergillus RB1 pada berbagai waktu inkubasi. Isolat diremajakan kemudian ditumbuhkan pada medium CMC cair dengan variasi lama inkubasi 0, 2, 4, 6, 8, 10, dan 12 hari dengan 3 ulangan. Filtrat kultur digunakan sebagai sumber ekstrak kasar enzim selulase, yang diuji aktivitasnya menggunakan metode DNS dengan pembandingan terhadap kurva standar glukosa. Pertumbuhan miselium dianalisis berdasarkan pengukuran berat kering biomassa pada setiap waktu inkubasi. Hasil penelitian menunjukkan bahwa aktivitas selulase Aspergillus RB1 meningkat seiring bertambahnya waktu inkubasi dan mencapai nilai tertinggi pada hari ke-4 sebesar 17,92 U/mL. Pola pertumbuhan miselium menunjukkan berat kering maksimum pada hari ke-4 sebesar 0,81 g, yang mengindikasikan fase pertumbuhan eksponensial sebagai fase optimum produksi enzim. Keselarasan antara puncak aktivitas selulase dan kurva tumbuh miselium menunjukkan bahwa lama inkubasi hari ke-4 merupakan waktu optimum untuk produksi selulase oleh Aspergillus RB1.
Co-Authors Adinda Eka Murti Setio Arif Rahman Hikam Aris Mumpuni Dwiana Muflihah Yulianti Fitriya Yuni Wulandari Nuniek Ina Ratnaningtyas Sri Lestari