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All Journal Sport and Fitness Journal METAMORFOSA Journal of Biological Sciences INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES E-Jurnal Medika Udayana JURNAL BIOMEDIK Public Health of Indonesia ISM (Intisari Sains Medis) : Jurnal Kedokteran Neurologico Spinale Medico Chirurgico IJAAM (Indonesian Journal of Anti-Aging Medicine) Jurnal Ilmiah Sangkareang Mataram Public Health and Preventive Medicine Archive Journal of Global Pharma Technology
Gde Ngurah Idraguna Pinatih
Bagian Ilmu Kedokteran Komunitas/Ilmu Kedokteran Pencegahan Fakultas Kedokteran Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Journal : IJAAM (Indonesian Journal of Anti-Aging Medicine)

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Administration of broccoli extract (Brassica oleracea var. italica) inhibited the increase of malondialdehyde level and the decrease of aortic endothelial cells in male wistar rats (Rattus norvegicus) exposed by cigarette smoke Hayatul Firi; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.53 KB)

Abstract

Introduction: In the environment, there are many free radicals for the body, one of them is cigarette smoke which can cause oxidative stress conditions and result in damage to body cells. The broccolis extract containing flavonoids, glucosinolates, vitamins C, E and beta-carotene which have a high antioxidant protective effect. Methods: An experimental post-test only control study was conducted using 36 males Wistar rats, aged 10-12 weeks, and 200-300 gr weight which were divided in 2 groups (control and treatment group). The broccoli extract (120 mg/ 200 grBB) was given to treatment group one hour before exposure to cigarette smoke. After 28 days of the treatment, the rats were taken for examination of MDA levels and underwent a surgery for aortic tissue for endothelial histopathology examination. Results: The Finding indicated that the data were in normal distribution through data analysis of the Shapiro Wilk test. Lavene’s test showed that both the data distribution and variety of both groups was homogenous (p>0.05). Through independent t-test, there were significant differences between the two groups on the value of MDA and aortic endothelium number (p <0.05) as the result of the comparison result of both groups. Conclusion: The administration of broccoli extract (Brassica oleracea var.italica) inhibits the increased levels of blood malondialdehyde and a decreased of aortic endothelial cells in Wistar male rats exposed to cigarette smoke.
Topical administration of deep sea shark liver oil (desslotm) inhibited mmp-1 expression in rat’s skin exposed to ultraviolet-B Bunge Dianmentari; Gede Ngurah Indraguna Pinatih; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.196 KB) | DOI: 10.36675/ijaam.v4i1.39

Abstract

Introduction: Squalene is one of strong antioxidants that can inhibit aging proses. However, its effect on the skin aging is still understudied. Therefore, the purpose of this study was to assess the efficacy of topical administration of Deep Sea Shark Liver Oil toward MMP-1 expression in Wistar rat’s (Rattus Norvegicus) skin exposed to UVB. Methods: An experimental randomized post-test only control group study was conducted using a total of 36 rats Wistar rats were used which were divided into 2 groups. The control group received placebo solution while the treatment group was treated by Deep Sea Shark Liver Oil solution. All groups were exposed to UVB with a total dose of 840 mJ/cm² for 4 weeks. Biopsy was conducted for skin sample collection for the examination expression of MMP-1. Results: Immunohistochemistry evaluation showed that the dermal MMP-1 expression in the control group was higher than in control group. According to the percentage of expression analysis, the mean of MMP-1 expression in control group were significantly higher (29.53%) compared to the treatment group (9.72%) (p=0.001). Conclusion: It can be concluded that the topical administration of Deep Sea Shark Liver Oil solution inhibited the expression of MMP-1 in rat’s skin exposed to UVB.
Administration of bali arabica (Coffea arabica) coffee extracts decreases abdominal fat and body weight in obese wistar rats (Rattus norvegicus) Fanny Rustandi; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

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Abstract

Introduction: The amount of obesity in the world in 2016 tripled compared to 1975 based on data from the World Health Organization (WHO). Previous research has shown that coffee has the potential to reduce weight and heavy abdominal fat. But there has been no research on extracts of Balinese arabica coffee beans. The purpose of this study was to prove that extracts of Balinese Arabica coffee beans can reduce weight and weight of abdominal fat in obese wistar male rats. Methods: This research is a study using Post-test only Control Group Design. The research subjects were 36 male white rats wistar (Rattus norvegicus) strain, healthy, aged 3-4 months and obese (Lee index> 0.3) divided into two groups, each amounting to 18 rats. The control group (P0) was the one who received placebo and the treatment group (P1) was given bali coffee bean extract 800mg / kgBW / day for 28 days. The variables observed were body weight, abdominal subcutaneous fat weight (BLS), and weight of visceral abdominal fat (BLV). In addition, the remaining food is measured every day. Results: The results showed the mean body weight P0 302.33 ± 6.22gram, P1 286.88 ± 4.85 gram (p <0.005). The mean BLS P0 was 0.61 ± 0.07 grams and P1 was 0.41 ± 0.08 grams (p <0.005). The mean BLV P0 5.55 ± 0.90 gram and P1 4.58 ± 0.60 gram (p <0.005). The mean feed remaining from the control group was 4.081 ± 0.57 grams and from the treatment group 6.07 ± 0.64 grams (p <0.005). Conclusion: Based on the above data it can be concluded that the administration of Bali coffee bean extract can reduce weight, weight of abdominal subcutaneous fat and heavy abdominal visceral fat in male wistar rats with obesity. In addition, it is suspected that extract of Bali coffee beans also reduces the amount of food intake.
Ethanol extract of red fruit (Pandanus conoideus lam.) improved lipid profile of dyslipidemic-male wistar rats (Rattus norvegicus) Sriwiyanti Sriwiyanti; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.88

Abstract

Introduction: Dyslipidemia is one of aging- related disease associated with unhealthy food such as high calories and fat. Pandanus Conoideus Lam. with high antioxidant capacity might ameliorates dyslipidemia phenotypes. This study aimed to prove that ethanol extract of red fruit improves lipid profiles of dyslipidemic-rats (Rattus norvegicus). Methods: This was an experimental study with a pretest-posttest control group design, using 14 wistar rats males. Rats were then divided into 2 groups (n= 7). The first group was treated with aquabidestilata 1 mL for 30 days (P0). The second group was treated with extract of red fruit ethanol of 168 mg/200 gr BW for 30 days (P1). Results: The control group (P0) treated with aquabidestilata, total cholesterol levels increased into 221.48 ± 3.64 mg/dl (p <0.05), triglyceride levels elevated into 132.92±3.01 mg/dl (p <0.05), HDL levels depleted into 22.83 ± 2.26 mg/dl (p <0.05), and LDL levels was increased from 76.77 ± 3.33 mg / dl to 78.63 ± 2.84 mg / dl (p <0.05). In contrast, the red fruit ethanol extract in treatment group (P1) experienced decrease on total cholesterol level into 118.41 ± 2.15 mg/dl (p <0.05), triglyceride levels into 91.81 ± 2.71 mg/dl (p <0.05), and LDL levels into 45.85 ± 2.59 mg/dl (p <0.05), and elevation on HDL levels from 25.77 ± 1.36 mg/dl to 55.76 ± 2.35 mg/dl (p <0.05). Conclusion: This study indicated that administration of ethanol extract of red fruit (Pandanus conoideus lam.) improved lipid profiles of dyslipidemic-rats (Rattus norvegicus).
Topical administration of deep sea shark liver oil (DESSLO™) inhibit Nuclear Factor-kappa Beta (NF-κB) expression in Wistar rats (Rattus Norvegicus) skin exposed to ultraviolet-B Devita Horax; A. A. G. P. Wiraguna; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.583 KB) | DOI: 10.36675/ijaam.v4i1.40

Abstract

Introduction: Repeated exposure of the skin to UV-B rays induce reactive oxygen species (ROS) formation that activates Nuclear Factor-kappa Beta (NF-κB) and stimulates pro- inflammatory cytokines expression which leads to premature aging of skin. Deep-sea shark liver oil contains ±80% squalene which could help neutralized ROS. Therefore, the purpose of this study was to assess the effect of topical deep sea shark liver oil toward NF-κB expression in UV-B-exposed on wistar rat (Rattus norvegicus) skin. Methods: A true experimental posttest-only control study was conducted using 36 Wistar rats which divided into 2 groups (n= 18). Control group was exposed to UV-B and treated with topical placebo basis of glycerin while the treatment group was treated with topical deep sea shark liver oil. A total of 840 mJ/cm2 UV-B exposure was given for 4 weeks. NF-κB expression was examined by immunohistochemistry. Results: The mean NF-κB expression was significantly higher in control group at 30.8±3.81 while the treatment group treated with topical deep sea shark liver oil has average expression at 10.4±1.98. The independent t-test showed a t-value of 20.090, and a p-value of 0.033. Conclusion: It can be concluded that topical deep sea shark liver oil inhibits the expression of NF-κB expression in UV-B-exposed Wistar rat (Rattus norvegicus) skin.
Selenium enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs Felix Kusmana; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.79

Abstract

Abstract Background: Exercise maintains health and prevent aging. However, excessive exercise causes overtraining syndrome. Selenium supplementation may increase cellular antioxidant activity, such as glutathione peroxidase (GPx), thioredoxin reductase (TRx), and selenoprotein P. This study aimed to prove that selenium-enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs.Methods: This experimental study used randomized pretest-posttest control group design. Subjects were 16 male Wistar rats, 16 weeks old, healthy, weighing 150-160 grams. Subjects were divided randomly into 2 groups (n=8), one group was treated with excessive physical training and 6.48 grams of ordinary eggs, and another group was treated with excessive physical training and 6.48 grams of selenium-enriched eggs for 14 days. Results: Post-test analysis showed that the mean of glutathione peroxidase level in the group given ordinary eggs was 45.90±8.11 mIU/mL, while the group given selenium-enriched eggs was 72.61±7.40 mIU/mL (p= 0.001). In addition, the mean of F2-Isoprostane level in the group given ordinary eggs was 6.37±1.34 ng/ml and the group given selenium-enriched eggs was 3.39±0.77 ng/ml (p= 0,001). Analysis of paired sample t-test revealed that in both groups, glutathione peroxidase levels were increase while F2-isoprostane levels were decrease. Conclusion: This study suggested that selenium-enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs. Key words: excessive physical training, F2-isoprostane, glutathione peroxidase, selenium-enriched eggs, Wistar rat.
Pomegranate extract (Punica granatum) not as effective as simvastatin in improving profile lipid in dyslipidemic male wistar rats (Rattus norvegicus) Ken Andrina Anindita; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.89

Abstract

Introduction: Dyslipidemia is one of the factors of the occurrence of heart disease and atherosclerosis. Simvastatin and Pomegranate (Punica granatum) work by improving the lipid profile. The aim of this study was to prove that red pomegranate extract (Punica granatum) improve lipid profile of male white rats that are dyslipidemic. Method: This research is a experimental study with randomized pre test and post test control group design, using 20 adult male white rats as sample. After 30 days, rats were divided into 2 study groups with 10 rats per group. The group was classified into two (P1 and P2). Prior to the treatment, the rats were examined the first diluted T-cholesterol, triglyceride, LDL and HDL levels as pretest data. Treatment was performed for 15 days. Results: The mean pretest HDL level in the simvastatin group was 23.84 ± 2.54 mg / dL and the red pomegranate extract group was 25.41 ± 2.34 mg / dL and p = 0.167. After treatment for 15 days, the mean posttest the red pomegranate extract group was 126.55 ± 3.09 mg / dL and p = 0,001. The mean posttest HDL level in the simvastatin group was 58.72 ± 5.21 mg / dL and the red pomegranate extract group was 40.56 ± 2.87 mg / dL and p = 0,001. Conclusion: The administration of red Punica granatum is not as effective as simvastatin in improving lipid profile in dyslipidemic rats. Although not as effective as simvastatin, red pomegranate extract can significantly improve lipid profile.
Co-Authors A. A. G. P. Wiraguna A. Santosa Adiputra, I Made Sudarma Anak Agung Ayu Ngurah Susraini Anak Agung Gde Putra Wiraguna Anak Agung Gde Putra Wiraguna Anak Agung Mas Putrawati Triningrat Ariastuti, Luh P. Ariesanti Tri Handayani, Ariesanti Tri Azri Hazwan Bunge Dianmentari Citra Anggraeny Devita Horax Dhumaranang, Hyangayu Dedari Dyah Pradnyaparmita Duarsa Fanny Rustandi Felicia Hartono Felix Kusmana Gede Putra IGN Gian Lisuari Adityasiwi Gina Septivani H Da Silva Hayatul Firi Herman Saputra I Dewa Nyoman Wibawa I Gede Widyatmika Pratama I Gusti Agung Triana Suharidewi I Gusti Ayu Sri Mahendra Dewi I Gusti Made Aman I Made Krisna Dinata I MADE MULIARTA . I Made Suganda Yatra I MADE SUTARGA I Putu Adiartha Griadhi I Putu Gede Adiatmika I Wayan Gede Artawan Eka Putra I Wayan Gede Jayanegara I Wayan Weta Ida Ayu Eugenia Natasha Blanco Oka Ida Bagus Ngurah IKG Muliartha Imtihanatun Najahah Jaeynisha Mathavan Kadek Tresna Adhi Ken Andrina Anindita Ketut Pramana Adiputra Ketut Tirtayasa Luh Made Indah Sri Handari Adiputra Luh Seri Ani Made Agus Kusumadjaja Makbullah - Marthin Alexander Rocky Muh. Irfan Muhammad Syairozi Hidayat Mulyanto Munawwarah, Muthiah Ni Made Ari Sukmandari Ni Made Septiari Maryani Ardi Ni Wayan Septarini Ni Wayan Trisnadewi, Ni Wayan NT Suryadhi Ompusunggu, Hillary Sekarningrum P., Gede Odi Bayu D. Pande Made Respa Dharma Syantih Pande Putu Januraga Pradnyaparamita, Dyah Putu Adhi Surya Pradana Rubagan Chelvam Samantha Celena Triadi Sawitri, Anak Agung Sagung Sriwiyanti Sriwiyanti Susy Purnawati Thivyadharshini Uvaraju Triyanto Nugroho Wahyuddin, Wahyuddin Widhayanthi, Trisna A. Wimpie I Pangkahila