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Gde Ngurah Idraguna Pinatih
Bagian Ilmu Kedokteran Komunitas/Ilmu Kedokteran Pencegahan Fakultas Kedokteran Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Journal : INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES

 1 
PHYTOCHEMICAL CONTENT AND ANTIOXIDANT ACTIVITY IN TRADISIONAL BALINESE BABI-GULING SPICES Indraguna Pinatih, G N; Suryadhi, NT; Santosa, A.; Muliartha, IKG
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 2 Mei 2011
Publisher : Udayana University

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Abstract

The traditional Balinese richly spiced swine-grill (Babi-guling) has been increasingly popular even among both the domestic and foreign tourists. The traditional grill is very unique in taste as it contains two main components, i.e. the pork and the unique Balinese spices, which probably could work antagonistically one against the other in affecting people health. The pork because of its rich content of saturated fatty acid is a risk of atherosclerosis, while the spices appear to be rich in antioxidatns. This is a preliminary atherosclerosis experimental study designed to screen qualitatively the phytochemical contents of the spices and to test their antioxidant activity and strength in different concentrations in vitro. The methods used included Willstater test, NaOH 10% test, Meyer test, Leiberman-Burchard test, test for saponin, and test for phenol. The antioxidant activity was estimated by using DPPH (2,2-diphenyl-1-pricrylhydrazyl) test. The treatment applied to the spice before testing was raw and cooked by heating it inside the pig’s body cavity. The concentrations of spices tested were 100, 1000 and 8000 ppm. The results of our study revealed that the phytochemical content of the spices were flavonoids, terpenoids and phenolic compounds, but saponin and alkaloids were not detected. The antioxidant activity was ineffective in concentration of 100 ppm (percentage of reduction < 50%), effective in concentration of 1000 ppm (percentage of reduction 50-60%), and too thick in concentration of 8000 ppm (percentage of reduction >100%. We inferred that the traditional Balinese richly spiced swine-grill contains flavanoids, terpenoids and phenol which can act as antioxidants in vitro, and the most effective concentrations to decrease the free radicals were between 1000-8000 ppm.
Co-Authors A. A. G. P. Wiraguna A. Santosa Adiputra, I Made Sudarma Anak Agung Ayu Ngurah Susraini Anak Agung Gde Putra Wiraguna Anak Agung Gde Putra Wiraguna Anak Agung Mas Putrawati Triningrat Ariastuti, Luh P. Ariesanti Tri Handayani, Ariesanti Tri Azri Hazwan Bunge Dianmentari Citra Anggraeny Devita Horax Dhumaranang, Hyangayu Dedari Dyah Pradnyaparmita Duarsa Fanny Rustandi Felicia Hartono Felix Kusmana Gede Putra IGN Gian Lisuari Adityasiwi Gina Septivani H Da Silva Hayatul Firi Herman Saputra I Dewa Nyoman Wibawa I Gede Widyatmika Pratama I Gusti Agung Triana Suharidewi I Gusti Ayu Sri Mahendra Dewi I Gusti Made Aman I Made Krisna Dinata I MADE MULIARTA . I Made Suganda Yatra I MADE SUTARGA I Putu Adiartha Griadhi I Putu Gede Adiatmika I Wayan Gede Artawan Eka Putra I Wayan Gede Jayanegara I Wayan Weta Ida Ayu Eugenia Natasha Blanco Oka Ida Bagus Ngurah IKG Muliartha Imtihanatun Najahah Jaeynisha Mathavan Kadek Tresna Adhi Ken Andrina Anindita Ketut Pramana Adiputra Ketut Tirtayasa Luh Made Indah Sri Handari Adiputra Luh Seri Ani Made Agus Kusumadjaja Makbullah - Marthin Alexander Rocky Muh. Irfan Muhammad Syairozi Hidayat Mulyanto Munawwarah, Muthiah Ni Made Ari Sukmandari Ni Made Septiari Maryani Ardi Ni Wayan Septarini Ni Wayan Trisnadewi, Ni Wayan NT Suryadhi Ompusunggu, Hillary Sekarningrum P., Gede Odi Bayu D. Pande Made Respa Dharma Syantih Pande Putu Januraga Pradnyaparamita, Dyah Putu Adhi Surya Pradana Rubagan Chelvam Samantha Celena Triadi Sawitri, Anak Agung Sagung Sriwiyanti Sriwiyanti Susy Purnawati Thivyadharshini Uvaraju Triyanto Nugroho Wahyuddin, Wahyuddin Widhayanthi, Trisna A. Wimpie I Pangkahila