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All Journal HAYATI Journal of Biosciences JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Hidrosfir Indonesia Teknologi Indonesia
Rini Riffiani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Environmental Science

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ISOLASI BAKTERI PENDEGRADASI PHENANTHRENE DARI BATANTA-SALAWATI RAJA AMPAT PAPUA Riffiani, Rini
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3153

Abstract

ABSTRACTIsolation of Bacteria Degrading Phenanthrene in Batanta- Salawati Districts Raja AmpatPapua. Polycyclic aromatic hydrocarbons (PAHs) are important environmental contaminantsin soil and water. These compounds have a potential risk to human health, as many of them arecarsinogenic and toxic to marine organisms such as diatome, gasthrophode, mussel, and fish.Phenanthrene is one of the hazardous hydrocarbon compounds. The purpose of this researchwas to characterize microbial strains from Batanta-Salawati Raja Ampat Papua Island and theirability to remove phenanthrene. Two isolates were identified at their physiological characteristicsbased on salinity tolerance, pH tolerance and the composition of nitrogen base. Molecularidentification based on 16S rRNA gene sequences indicated that bacteria had the highestsimilarity with Rhodobacteraceae bacterium F9 and Roseobacter sp. RW 37.Rhodobacteraceae bacterium F9 could grow optimum on ONR7a media with 5% salinity andat pH of 5-7,5 while Roseobacter sp. RW 37 could grow optimum on ONR7a media with 2%salinity and at pH of 6,2-7,5.Key words: Phenanthrene, physiological characteristic, molecular identification, Raja Ampat
PENAPISAN MIKROBA LAUT PEROMBAK SENYAWA NITRIL DAN PROTEIN YANG DIISOLASI DARI SPONS DI PERAIRAN TERNATE Riffiani, Rini; Sulistinah, Nunik
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3143

Abstract

ABSTRACTScreening of Nitrile and Protein-Degrading Marine Bacteria Isolated from Sponge in TernateSea Water. Thirty three marine bacteria have been isolated from marine sponge in Ternate byenrichment culture. Screening bacteria-degrading nitrile was done by microtitter plate methodbased on growth ability tested by Iodonitrotetazolium chloride. Product of nitrile degradationwas determined by Gas Chromatography (GC) and the potential bacteria-degrading proteinwas also screened by using selected media which contained casein. The results showed thattwenty one isolates were able to show the clearing zone in selected media. Five isolatescapable of utilizing acetamide as the sole source of carbon and nitrogen. Acetate and ammoniaproduced for hydrolysis acetonitrile by using resting cell of Lysobacter sp.Key words: Nitrile, bacterium, sponge, Ternate
PENGEMBANGAN SISTEM DETEKSI SENYAWA SIANOGEN DALAM UBI KAYU (MANIHOT ESCULENTA CRANTZ) DENGAN PENDEKATAN ENZIMATIS Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.332

Abstract

Picrate paper test kit for the semiâ??quantitative determination of cyanogenic potential was developed in thisexperiment. The method is relatively simple, easy to use and might be applicable in the field by unskilled person.Paper test was attached on tubes containing sample (100 mg) in aquadest (0,5 mL) and then was immediatelycovered tightly and incubated overnight at room temperature. The colour of picrate paper test changed graduallytowards reddish brown, and its colour was compared with standart colour chart which included 0-800 ppm cyanidethat was also developed in this study. The reddish brown colour of paper test was correlated with cyanideconcentration on the sample. In order to obtain a more accurate detection of cyanogenic compound the paper testwas eluted with 5 mL water or aquadest and the absorbance was measured at 510 nm.Keywords: cyanogenic potential, picrate paper test, semi-quantitative method, simple method, cassava (Manihotesculenta Cranz)
PENGUNCILAN GEN PENYANDI ENZIM NITRILASE ENAM ISOLAT BAKTERI UNGGULAN Riffiani, Rini; Sulistinah, Nunik; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2155

Abstract

Indonesia sebagai negara tropis memiliki biodiversitas yang sangat tinggi. Keanekaragaman hayati ini diperkirakan mencerminkan keanekaragaman kimiawi sekaligus keragaman genetik yang dapat dimanfaatkan untuk mencari biokatalis baru. Enam isolat bakteri yaitu GLB5, LP3, TPIK, MICC, 23A2, dan 23A2 telah diisolasi dari berbagai limbah industri dan mempunyai potensi sebagai pendegradasi nitril.  Pengucilan, identifikasi dan purifikasi gen penyandi enzim nitrilase dari keenam isolat bakteri tersebut  telah dilakukan. Dari kegiatan penelitian ini 3 isolat bakteri unggulan, yaitu GLB5, LP3, dan TPIK teridentifikasi sebagai Rhodococcus pyridinivorans, sedangkan  MICC teridentifikasi sebagai Bacillus substilis, 23A2 teridentifikasi sebagai Brevibacillus brevis, dan 26A2 teridentifikasi sebagai Microbacterium oxydans. Peta untaian basa nukleutida dari gen penyandi enzim nitrilase dari ketiga isolat yaitu GLB5, LP3, dan TPIK telah terpetakan dengan ukuran gen nitrilase sebesar 960 bp. Hasil analisis dengan BLASTN memperlihatkan bahwa fragmen gen nitrilase yang diamplifikasi dengan primer Nit1101F dan Nit1101R mempunyai homologi yang tinggi terhadap Rhodococcus rhodochrous strain tg1-A6 nitrilase gen dengan persentase kesamaan sebesar 96% . Kata Kunci: Gen, isolasi, nitril, degradasi, enzim 
PENGGUNAAN MIKROALGA CHLORELLA PYRENOIDOSA CHICK AMOBIL UNTUK MENINGKATKAN KUALITAS AIR DALAM AKUAKULTUR Riffiani, Rini
Jurnal Hidrosfir Indonesia Vol 5, No 2 (2010)
Publisher : Jurnal Hidrosfir Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.832 KB)

Abstract

Immobilized microalgae Chlorella pyrenoidosa was applied initially to nutrient and heavy metal removal of wastewater. Immobilized microalgae using alginate was then developed for aquaculture application, such as controlling fish water culture quality to uptake concentration of ammonium, nitrate and to increase the oxygen level in water. During immobilization, algal cell maintain their respiratory and photosynthetic activities as that cell in the normal condition. The objective of this research was to examine the role of C. pyrenoidosa immobile on controlling the water quality by measuring ammonium, nitrate, and dissolved oxygen content. Five aquariums consisted of 40 litres of water were filled with 20 Nile Tilapia (Oreothromis niloticus) with the average weight between 1.6 and 1.7 g. The immobile algae cell were packaged in two nillon porus bag (pore size was 2x3 mm in diameter) and each immobile cell had 4 millimeter in diameter. Each aquarium was added with 3000, 4000, 5000, and 6000 of immobile cell. The treatment had 2 replicates. The results showed that the aquarium filled with 4000 beads of immobile cell gave the best result. The ammonium content on the water decrease 6,626 ppm/day, nitrate content on the water decreased 13.99 ppm/ day, soluble oxygen raised 0.766 per day and fish biomass raised 1.56 g/fish for 15 days.
BAKTERI PENGHASIL BIOSURFAKTAN YANG DIISOLASI DARI PULAU LAKI KEPULAUAN SERIBU Riffiani, Rini
Jurnal Hidrosfir Indonesia Vol 5, No 3 (2010)
Publisher : Jurnal Hidrosfir Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.051 KB)

Abstract

Biosurfactants are the surface-active molecules synthesized by microorganisms. The microbial surfactant were interesting because of the biodegradable and have many application in industry. With the advantage of environmental compatibility, the demand for biosurfactants has beensteadily increasing and may eventually replace their chemically synthesized counterparts. Marine biosurfactants produced by some marine microorganisms have been paid more attention particularly for the bioremediation of the sea polluted by crude oil. The aim of this research isto screening microorganisms that produce biosurfactant from Pulau Laki, Kepulauan Seribu, Jakarta. The isolate which produce biosurfactant showed by clear ring zone on ONR7a crude oil medium. Three isolates were identified their characteristics based on the composition of nitrogenbase. Molecular identification based on 16S rRNA gene sequences indicated that bacteria had the highest similarity with Marinobacter satoriniensis strain NKSGI, Paracoccus sp and Pseudomonas sp.
Peningkatan Kualitas Akuakultur Menggunakan Teknologi Biofilter Mikroalga Imobil Rini Riffiani
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.831 KB) | DOI: 10.20884/1.mib.2009.26.3.165

Abstract

Scrutinize the Taxonomical Identity of Green Edible Russula from Sulawesi (Indonesia) Nurhayat, Oktan Dwi; Putra, Ivan Permana; Riffiani, Rini; Taridala, Sitti Aida Adha; Arif, Zulhan
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.436-444

Abstract

Russula Pers. (Basidiomycota) is recognized as one of the most widely distributed macrofungi globally. This genus includes several edible species with distinct color variations, such as R. cyanoxantha. However, in Indonesia, knowledge of this species remains limited as colourful mushrooms are often mistakenly considered poisonous. During a fungus expedition in Southeast Sulawesi, we collected a green edible wild mushroom consumed by locals. Our study aimed to verify the taxonomic classification of our specimens through morphological and molecular analysis. The fresh basidiomata were examined based on their macroscopic and microscopic characteristics. Molecular analysis using ITS 1/2 sequences was conducted to construct a phylogenetic tree. Our findings identified the green mushroom as R. cyanoxantha (Schaeff.) Fr. (Synonym: R. lilacina). Morphologically, our specimens can be distinguished from R. virescens by their smooth cap. They differ from R. aeruginea and R. heterophylla by having flexible, persistent gills. Additionally, our R. cyanoxantha specimen BO24636 can be separated from R. dinghuensis and R. subpallidirosea by their forked gills near the stalk. A BLAST comparison revealed a 96% similarity to R. lilacina from Thailand as the closest match. The phylogenetic tree (Maximum Likelihood) placed our specimens within the R. lilacina group (BS value 99%). Our research contributes to the taxonomic understanding of this edible wild green macrofungi in Indonesia, and future studies should explore its nutritional composition.
Co-Authors BAMBANG SUNARKO Ivan Permana Putra NUNIK SULISTINAH Nurhayat, Oktan Dwi Taridala, Sitti Aida Adha